Collecting saliva and measuring salivary cortisol and alpha-amylase in frail community residing older adults via family caregivers.

Nancy A. Hodgson, Douglas A. Granger

Research output: Contribution to journalArticle

Abstract

Salivary measures have emerged in bio-behavioral research that are easy-to-collect, minimally invasive, and relatively inexpensive biologic markers of stress. This article we present the steps for collection and analysis of two salivary assays in research with frail, community residing older adults-salivary cortisol and salivary alpha amylase. The field of salivary bioscience is rapidly advancing and the purpose of this presentation is to provide an update on the developments for investigators interested in integrating these measures into research on aging. Strategies are presented for instructing family caregivers in collecting saliva in the home, and for conducting laboratory analyses of salivary analytes that have demonstrated feasibility, high compliance, and yield quality specimens. The protocol for sample collection includes: (1) consistent use of collection materials; (2) standardized methods that promote adherence and minimize subject burden; and (3) procedures for controlling certain confounding agents. We also provide strategies for laboratory analyses include: (1) saliva handling and processing; (2) salivary cortisol and salivary alpha amylase assay procedures; and (3) analytic considerations.

Original languageEnglish (US)
JournalJournal of Visualized Experiments
Issue number82
StatePublished - 2013

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Salivary alpha-Amylases
Cortisol
Amylases
alpha-Amylases
Saliva
Caregivers
Hydrocortisone
Assays
Behavioral research
Behavioral Research
Aging of materials
Research
Compliance
Processing
Biomarkers
Research Personnel

ASJC Scopus subject areas

  • Medicine(all)

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Collecting saliva and measuring salivary cortisol and alpha-amylase in frail community residing older adults via family caregivers. / Hodgson, Nancy A.; Granger, Douglas A.

In: Journal of Visualized Experiments, No. 82, 2013.

Research output: Contribution to journalArticle

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