Cochlear vibrations in mice with altered stereociliary mechanics

James B. Dewey, Anping Xia, Patrick D. Raphael, Ulrich Mueller, Inna A. Belyantseva, Brian E. Applegate, John S. Oghalai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The mechanical properties of outer hair cell (OHC) stereocilia are critical for the transduction currents that drive OHC force generation. Stereociliary properties therefore play a prominent role in shaping "active" cochlear mechanics. However, it is less clear whether OHC stereocilia also directly influence passive cochlear mechanics due to contributions to the overall stiffness or coupling within the cochlear partition. To examine this, we used volumetric optical coherence tomography vibrometry to measure vibrations from the cochlear apex of wild type (WT) mice and mice with genetically-Altered stereociliary properties. The latter included salsa mice, which have a Cdh23 missense mutation and progressively lose their stereociliary tip links, and TRIOBP-4/5 deficient TriobpΔex8/Δex8 mice, which lack stereociliary rootlets and suffer progressive stereociliary degeneration. Consistent with the absence of normal mechanotransduction in salsa and TriobpΔex8/Δex8 mice, cochlear vibrations from these mice exhibited no nonlinear gain and largely resembled vibrations from dead WT mice. However, examination of vibrations in dead salsa and TriobpΔex8/Δex8 mice also revealed a downward shift in the high-frequency portions of the vibratory gain and phase curves, as well as a steeper phase slope in TriobpΔex8/Δex8 mice, relative to dead WT mice. Though subtle, these effects suggest that OHC stereociliary bundles contribute to longitudinal coupling within the cochlear partition, likely via their interactions with the tectorial membrane.

Original languageEnglish (US)
Title of host publicationTo the Ear and Back Again - Advances in Auditory Biophysics
Subtitle of host publicationProceedings of the 13th Mechanics of Hearing Workshop
PublisherAmerican Institute of Physics Inc.
Volume1965
ISBN (Electronic)9780735416703
DOIs
StatePublished - May 31 2018
Event13th Mechanics of Hearing Workshop: To the Ear and Back Again - Advances in Auditory Biophysics, MoH 2017 - St. Catharines, Canada
Duration: Jun 19 2017Jun 24 2017

Other

Other13th Mechanics of Hearing Workshop: To the Ear and Back Again - Advances in Auditory Biophysics, MoH 2017
CountryCanada
CitySt. Catharines
Period6/19/176/24/17

Fingerprint

mice
vibration
hair
partitions
degeneration
mutations
bundles
stiffness
apexes
tomography
examination
mechanical properties
slopes
membranes
shift
curves

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Dewey, J. B., Xia, A., Raphael, P. D., Mueller, U., Belyantseva, I. A., Applegate, B. E., & Oghalai, J. S. (2018). Cochlear vibrations in mice with altered stereociliary mechanics. In To the Ear and Back Again - Advances in Auditory Biophysics: Proceedings of the 13th Mechanics of Hearing Workshop (Vol. 1965). [080003] American Institute of Physics Inc.. https://doi.org/10.1063/1.5038487

Cochlear vibrations in mice with altered stereociliary mechanics. / Dewey, James B.; Xia, Anping; Raphael, Patrick D.; Mueller, Ulrich; Belyantseva, Inna A.; Applegate, Brian E.; Oghalai, John S.

To the Ear and Back Again - Advances in Auditory Biophysics: Proceedings of the 13th Mechanics of Hearing Workshop. Vol. 1965 American Institute of Physics Inc., 2018. 080003.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Dewey, JB, Xia, A, Raphael, PD, Mueller, U, Belyantseva, IA, Applegate, BE & Oghalai, JS 2018, Cochlear vibrations in mice with altered stereociliary mechanics. in To the Ear and Back Again - Advances in Auditory Biophysics: Proceedings of the 13th Mechanics of Hearing Workshop. vol. 1965, 080003, American Institute of Physics Inc., 13th Mechanics of Hearing Workshop: To the Ear and Back Again - Advances in Auditory Biophysics, MoH 2017, St. Catharines, Canada, 6/19/17. https://doi.org/10.1063/1.5038487
Dewey JB, Xia A, Raphael PD, Mueller U, Belyantseva IA, Applegate BE et al. Cochlear vibrations in mice with altered stereociliary mechanics. In To the Ear and Back Again - Advances in Auditory Biophysics: Proceedings of the 13th Mechanics of Hearing Workshop. Vol. 1965. American Institute of Physics Inc. 2018. 080003 https://doi.org/10.1063/1.5038487
Dewey, James B. ; Xia, Anping ; Raphael, Patrick D. ; Mueller, Ulrich ; Belyantseva, Inna A. ; Applegate, Brian E. ; Oghalai, John S. / Cochlear vibrations in mice with altered stereociliary mechanics. To the Ear and Back Again - Advances in Auditory Biophysics: Proceedings of the 13th Mechanics of Hearing Workshop. Vol. 1965 American Institute of Physics Inc., 2018.
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