TY - GEN
T1 - Cochlear vibrations in mice with altered stereociliary mechanics
AU - Dewey, James B.
AU - Xia, Anping
AU - Raphael, Patrick D.
AU - Mueller, Ulrich
AU - Belyantseva, Inna A.
AU - Applegate, Brian E.
AU - Oghalai, John S.
N1 - Funding Information:
This project was supported by NIDCD/NIH grants DC016211 (J.B. Dewey), DC014450, DC013774, and DC010363 (J.S. Oghalai), as well as funds from the NIDCD/NIH Intramural Research Program DC000039 (T.B. Friedman).
Publisher Copyright:
© 2018 Author(s).
PY - 2018/5/31
Y1 - 2018/5/31
N2 - The mechanical properties of outer hair cell (OHC) stereocilia are critical for the transduction currents that drive OHC force generation. Stereociliary properties therefore play a prominent role in shaping "active" cochlear mechanics. However, it is less clear whether OHC stereocilia also directly influence passive cochlear mechanics due to contributions to the overall stiffness or coupling within the cochlear partition. To examine this, we used volumetric optical coherence tomography vibrometry to measure vibrations from the cochlear apex of wild type (WT) mice and mice with genetically-Altered stereociliary properties. The latter included salsa mice, which have a Cdh23 missense mutation and progressively lose their stereociliary tip links, and TRIOBP-4/5 deficient TriobpΔex8/Δex8 mice, which lack stereociliary rootlets and suffer progressive stereociliary degeneration. Consistent with the absence of normal mechanotransduction in salsa and TriobpΔex8/Δex8 mice, cochlear vibrations from these mice exhibited no nonlinear gain and largely resembled vibrations from dead WT mice. However, examination of vibrations in dead salsa and TriobpΔex8/Δex8 mice also revealed a downward shift in the high-frequency portions of the vibratory gain and phase curves, as well as a steeper phase slope in TriobpΔex8/Δex8 mice, relative to dead WT mice. Though subtle, these effects suggest that OHC stereociliary bundles contribute to longitudinal coupling within the cochlear partition, likely via their interactions with the tectorial membrane.
AB - The mechanical properties of outer hair cell (OHC) stereocilia are critical for the transduction currents that drive OHC force generation. Stereociliary properties therefore play a prominent role in shaping "active" cochlear mechanics. However, it is less clear whether OHC stereocilia also directly influence passive cochlear mechanics due to contributions to the overall stiffness or coupling within the cochlear partition. To examine this, we used volumetric optical coherence tomography vibrometry to measure vibrations from the cochlear apex of wild type (WT) mice and mice with genetically-Altered stereociliary properties. The latter included salsa mice, which have a Cdh23 missense mutation and progressively lose their stereociliary tip links, and TRIOBP-4/5 deficient TriobpΔex8/Δex8 mice, which lack stereociliary rootlets and suffer progressive stereociliary degeneration. Consistent with the absence of normal mechanotransduction in salsa and TriobpΔex8/Δex8 mice, cochlear vibrations from these mice exhibited no nonlinear gain and largely resembled vibrations from dead WT mice. However, examination of vibrations in dead salsa and TriobpΔex8/Δex8 mice also revealed a downward shift in the high-frequency portions of the vibratory gain and phase curves, as well as a steeper phase slope in TriobpΔex8/Δex8 mice, relative to dead WT mice. Though subtle, these effects suggest that OHC stereociliary bundles contribute to longitudinal coupling within the cochlear partition, likely via their interactions with the tectorial membrane.
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U2 - 10.1063/1.5038487
DO - 10.1063/1.5038487
M3 - Conference contribution
AN - SCOPUS:85048210852
T3 - AIP Conference Proceedings
BT - To the Ear and Back Again - Advances in Auditory Biophysics
A2 - Bergevin, Christopher
A2 - Puria, Sunil
PB - American Institute of Physics Inc.
T2 - 13th Mechanics of Hearing Workshop: To the Ear and Back Again - Advances in Auditory Biophysics, MoH 2017
Y2 - 19 June 2017 through 24 June 2017
ER -