Coagulation factor XIII-A. A flow cytometric intracellular marker in the classification of acute myeloid leukemias

János Kappelmayer, Ágnes Simon, Éva Katona, Attila Szanto, Laszlo Nagy, Attila Kiss, Csongor Kiss, László Muszbek

Research output: Contribution to journalArticle

Abstract

The association of coagulation factors with leukocytes have been demonstrated in several previous studies. This study was designed to study the sensitivity and specificity of factor XIII subunitA (FXIII-A) labelling in cultured myeloblastic and monoblastic cell lines and to investigate the intracytoplasmic expression of FXIII-A in de novo acute myeloid leukemia (AML) samples. Myeloblastic and a monoblastic cell lines were cultured and investigated for lineage specific maturation markers and FXIII-A expression. Furthermore, FXIII-A expression was investigated in 12 normal samples (7 bone marrow and 5 peripheral blood), 86 de novo AML samples and 6 chronic myelomonocytic leukemia (CMML) samples. In the monoblastic MonoMac6 cell line the appearance of FXIII-A preceded that of CD14 while it remained negative in the myeloblastic PLB-985 cell line throughout its maturation period. Among the AML samples the average frequency of FXIII-A positive cells in myeloblastic leukemia samples was below 10%, while in M4 and M5 AML samples it was above 50% and was significantly higher than the generally used CD14 marker (p<0.0001). In the AML M4 and M5 cases, FXIII-A proved sensitive for the identification of monoblasts. FXIII-A can be considered as a reliable intracytoplasmic marker for the monocytic and megakaryocytic series and its presence is highly predictive for mono- and megakaryocytic AML and for CMML.

Original languageEnglish (US)
Pages (from-to)454-459
Number of pages6
JournalThrombosis and Haemostasis
Volume94
Issue number2
DOIs
StatePublished - Aug 1 2005
Externally publishedYes

Fingerprint

Factor XIII
Acute Myeloid Leukemia
Leukemia, Myelomonocytic, Acute
Leukemia, Myelomonocytic, Chronic
Leukemia, Monocytic, Acute
Cell Line
Monocyte-Macrophage Precursor Cells
Blood Coagulation Factors
Leukemia
Leukocytes
Bone Marrow
Sensitivity and Specificity

Keywords

  • Acute myeloid leukemia classification
  • Factor XIII
  • Flow cytometry

ASJC Scopus subject areas

  • Hematology

Cite this

Coagulation factor XIII-A. A flow cytometric intracellular marker in the classification of acute myeloid leukemias. / Kappelmayer, János; Simon, Ágnes; Katona, Éva; Szanto, Attila; Nagy, Laszlo; Kiss, Attila; Kiss, Csongor; Muszbek, László.

In: Thrombosis and Haemostasis, Vol. 94, No. 2, 01.08.2005, p. 454-459.

Research output: Contribution to journalArticle

Kappelmayer, János ; Simon, Ágnes ; Katona, Éva ; Szanto, Attila ; Nagy, Laszlo ; Kiss, Attila ; Kiss, Csongor ; Muszbek, László. / Coagulation factor XIII-A. A flow cytometric intracellular marker in the classification of acute myeloid leukemias. In: Thrombosis and Haemostasis. 2005 ; Vol. 94, No. 2. pp. 454-459.
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