CO2-induced c-Fos expression in brainstem preprotachykinin mRNA containing neurons

Gina Pete, Serdia O. Mack, Musa A. Haxhiu, Simon Walbaum, Estelle B Gauda

Research output: Contribution to journalArticle

Abstract

Tachykinin peptides are found in brainstem regions involved in central chemoreception and they may play a modulatory role in ventilatory response to hypercapnia. We determined whether tachykinin peptide containing neurons are activated by CO2 by combining in situ hybridization and immunohistochemistry (IHH). Experiments were performed in 21-day-old rats exposed to 12% CO2 for 1 h. c-Fos expression was identified by IHH on free floating sections (40 μm) that were mounted and then hybridized with anti-sense 35S labeled ribonucleotide probe of the rat preprotachykinin A (PPT-A) gene. Sections were analyzed for expression of the PPT-A gene, c-Fos protein and colocalization of PPT-A gene with c-Fos protein. Within the chemosensory region of the nucleus tractus solitarius (nTS), 19% of c-Fos positive cells expressed PPT-A mRNA after hypercapnic loading. In medullary raphe nuclei, 64% of c-Fos positive cells expressed the PPT-A gene after exposure to CO2, while 21% of c-Fos labeled neurons in parapyramidal nuclei also expressed PPT-A mRNA. These results indicate that a subpopulation of CO2 activated neurons within the nTS and in the parapyramidal and midline regions of the ventral aspect of the medulla oblongata express the PPT-A gene, suggesting that these are substance P- or neurokinin A-containing neurons. Furthermore, these peptides may play a role in modulation of respiratory and cardiovascular responses to changes in CO2/H+ content of the extracellular fluid.

Original languageEnglish (US)
Pages (from-to)265-274
Number of pages10
JournalRespiratory Physiology and Neurobiology
Volume130
Issue number3
DOIs
StatePublished - 2002

Fingerprint

Brain Stem
Neurons
Messenger RNA
Proto-Oncogene Proteins c-fos
Tachykinins
Solitary Nucleus
Genes
Peptides
Immunohistochemistry
Neurokinin A
Ribonucleotides
Medulla Oblongata
Raphe Nuclei
Hypercapnia
Extracellular Fluid
Substance P
preprotachykinin
In Situ Hybridization

Keywords

  • Chemosensitivity, central, CO
  • Control of breathing, c-Fos expression
  • Mammals, rat
  • Mediators, neurokinin A, substance P
  • Tachykinin, neurons, activated by CO

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Neuroscience(all)

Cite this

CO2-induced c-Fos expression in brainstem preprotachykinin mRNA containing neurons. / Pete, Gina; Mack, Serdia O.; Haxhiu, Musa A.; Walbaum, Simon; Gauda, Estelle B.

In: Respiratory Physiology and Neurobiology, Vol. 130, No. 3, 2002, p. 265-274.

Research output: Contribution to journalArticle

Pete, Gina ; Mack, Serdia O. ; Haxhiu, Musa A. ; Walbaum, Simon ; Gauda, Estelle B. / CO2-induced c-Fos expression in brainstem preprotachykinin mRNA containing neurons. In: Respiratory Physiology and Neurobiology. 2002 ; Vol. 130, No. 3. pp. 265-274.
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abstract = "Tachykinin peptides are found in brainstem regions involved in central chemoreception and they may play a modulatory role in ventilatory response to hypercapnia. We determined whether tachykinin peptide containing neurons are activated by CO2 by combining in situ hybridization and immunohistochemistry (IHH). Experiments were performed in 21-day-old rats exposed to 12{\%} CO2 for 1 h. c-Fos expression was identified by IHH on free floating sections (40 μm) that were mounted and then hybridized with anti-sense 35S labeled ribonucleotide probe of the rat preprotachykinin A (PPT-A) gene. Sections were analyzed for expression of the PPT-A gene, c-Fos protein and colocalization of PPT-A gene with c-Fos protein. Within the chemosensory region of the nucleus tractus solitarius (nTS), 19{\%} of c-Fos positive cells expressed PPT-A mRNA after hypercapnic loading. In medullary raphe nuclei, 64{\%} of c-Fos positive cells expressed the PPT-A gene after exposure to CO2, while 21{\%} of c-Fos labeled neurons in parapyramidal nuclei also expressed PPT-A mRNA. These results indicate that a subpopulation of CO2 activated neurons within the nTS and in the parapyramidal and midline regions of the ventral aspect of the medulla oblongata express the PPT-A gene, suggesting that these are substance P- or neurokinin A-containing neurons. Furthermore, these peptides may play a role in modulation of respiratory and cardiovascular responses to changes in CO2/H+ content of the extracellular fluid.",
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AU - Mack, Serdia O.

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AU - Gauda, Estelle B

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AB - Tachykinin peptides are found in brainstem regions involved in central chemoreception and they may play a modulatory role in ventilatory response to hypercapnia. We determined whether tachykinin peptide containing neurons are activated by CO2 by combining in situ hybridization and immunohistochemistry (IHH). Experiments were performed in 21-day-old rats exposed to 12% CO2 for 1 h. c-Fos expression was identified by IHH on free floating sections (40 μm) that were mounted and then hybridized with anti-sense 35S labeled ribonucleotide probe of the rat preprotachykinin A (PPT-A) gene. Sections were analyzed for expression of the PPT-A gene, c-Fos protein and colocalization of PPT-A gene with c-Fos protein. Within the chemosensory region of the nucleus tractus solitarius (nTS), 19% of c-Fos positive cells expressed PPT-A mRNA after hypercapnic loading. In medullary raphe nuclei, 64% of c-Fos positive cells expressed the PPT-A gene after exposure to CO2, while 21% of c-Fos labeled neurons in parapyramidal nuclei also expressed PPT-A mRNA. These results indicate that a subpopulation of CO2 activated neurons within the nTS and in the parapyramidal and midline regions of the ventral aspect of the medulla oblongata express the PPT-A gene, suggesting that these are substance P- or neurokinin A-containing neurons. Furthermore, these peptides may play a role in modulation of respiratory and cardiovascular responses to changes in CO2/H+ content of the extracellular fluid.

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