Co-infusion of donor bone marrow with host mesenchymal stem cells treats GVHD and promotes vascularized skin allograft survival in rats

Ali Emre Aksu, Elaine Horibe, Justin Sacks, Ryosuke Ikeguchi, Jeremy Breitinger, Merissa Scozio, Jignesh Unadkat, Maryam Feili-Hariri

Research output: Contribution to journalArticle

Abstract

We investigated the effect of autologous mesenchymal stem cells (MSC) on multiple unmodified donor bone marrow (BM) infusions and vascularized skin graft outcome. BM-derived rat MSC were examined for phenotype and function. MSC/MSC-conditioned-medium suppressed IFN-γ production by T cells and modified DC function. Infusions of MSC with one-time BM improved vascularized skin graft survival, while with one-two-times BM reversed graft versus host disease (GVHD). Mixed chimerism was enhanced in recipients given two-four-times BM with MSC infusions. Interestingly, four-times BM infusions with MSC delayed GVHD onset, reduced host tissue damage and enhanced vascularized skin allograft survival compared to four-times BM alone. These data demonstrate that, the co-infusion of MSC with unmodified BM limit the toxicity of allogeneic BM transplantation, enhance mixed chimerism and improve vascularized skin graft survival. These findings provide insights for the development of autologous MSC-based BM transplantation and prevention of graft rejection or treatment of autoimmunity.

Original languageEnglish (US)
Pages (from-to)348-358
Number of pages11
JournalClinical Immunology
Volume127
Issue number3
DOIs
StatePublished - Jun 1 2008
Externally publishedYes

Keywords

  • Bone marrow
  • GVHD
  • Mesenchymal stem cells
  • Rat
  • Vascularized skin

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Aksu, A. E., Horibe, E., Sacks, J., Ikeguchi, R., Breitinger, J., Scozio, M., Unadkat, J., & Feili-Hariri, M. (2008). Co-infusion of donor bone marrow with host mesenchymal stem cells treats GVHD and promotes vascularized skin allograft survival in rats. Clinical Immunology, 127(3), 348-358. https://doi.org/10.1016/j.clim.2008.02.003