Co-immunization with plasmid IL-12 generates a strong T-cell memory response in mice

Michael Anand Chattergoon, Vera Saulino, Jason P. Shames, Jamie Stein, Luis J. Montaner, David B. Weiner

Research output: Contribution to journalArticle

Abstract

Plasmid encoded exogenous IL-12 delivered as a DNA vaccine adjuvant has been shown to improve vaccine-induced immunity. In particular, pIL-12 greatly improves antigen (Ag)-specific cytotoxic tlymphocyte (CTL) activity in immunized mice. The longevity of this response has not previously been studied in detail. We have studied the effect of co-immunization with pIL-12 on HIV gp160 and Influenza A Hemeagglutinnin-specific memory immune responses. Mice co-immunized with pIL-12 and plasmid encoded antigens maintained a greater memory response than those immunized with the plasmid antigen alone which could be measured at least 6 months after vaccination. Further, this translated to an improved outcome after challenge of long term rested mice that were previously immunized. The strength of the immune response as well as the number of Ag-specific T-cells is proportional to the number of Ag-specific cells primed by the vaccination regimen.

Original languageEnglish (US)
Pages (from-to)1744-1750
Number of pages7
JournalVaccine
Volume22
Issue number13-14
DOIs
StatePublished - Apr 16 2004
Externally publishedYes

Fingerprint

interleukin-12
Interleukin-12
Immunization
plasmids
immunization
Plasmids
T-lymphocytes
antigens
T-Lymphocytes
Antigens
mice
HIV Envelope Protein gp160
Vaccination
vaccination
immune response
vaccine adjuvants
DNA Vaccines
recombinant vaccines
influenza
Human Influenza

Keywords

  • Adjuvant
  • IL-12
  • Vaccine

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Chattergoon, M. A., Saulino, V., Shames, J. P., Stein, J., Montaner, L. J., & Weiner, D. B. (2004). Co-immunization with plasmid IL-12 generates a strong T-cell memory response in mice. Vaccine, 22(13-14), 1744-1750. https://doi.org/10.1016/j.vaccine.2004.01.036

Co-immunization with plasmid IL-12 generates a strong T-cell memory response in mice. / Chattergoon, Michael Anand; Saulino, Vera; Shames, Jason P.; Stein, Jamie; Montaner, Luis J.; Weiner, David B.

In: Vaccine, Vol. 22, No. 13-14, 16.04.2004, p. 1744-1750.

Research output: Contribution to journalArticle

Chattergoon, MA, Saulino, V, Shames, JP, Stein, J, Montaner, LJ & Weiner, DB 2004, 'Co-immunization with plasmid IL-12 generates a strong T-cell memory response in mice', Vaccine, vol. 22, no. 13-14, pp. 1744-1750. https://doi.org/10.1016/j.vaccine.2004.01.036
Chattergoon, Michael Anand ; Saulino, Vera ; Shames, Jason P. ; Stein, Jamie ; Montaner, Luis J. ; Weiner, David B. / Co-immunization with plasmid IL-12 generates a strong T-cell memory response in mice. In: Vaccine. 2004 ; Vol. 22, No. 13-14. pp. 1744-1750.
@article{9ad1feeffff24cdf8543395f00f2a5a0,
title = "Co-immunization with plasmid IL-12 generates a strong T-cell memory response in mice",
abstract = "Plasmid encoded exogenous IL-12 delivered as a DNA vaccine adjuvant has been shown to improve vaccine-induced immunity. In particular, pIL-12 greatly improves antigen (Ag)-specific cytotoxic tlymphocyte (CTL) activity in immunized mice. The longevity of this response has not previously been studied in detail. We have studied the effect of co-immunization with pIL-12 on HIV gp160 and Influenza A Hemeagglutinnin-specific memory immune responses. Mice co-immunized with pIL-12 and plasmid encoded antigens maintained a greater memory response than those immunized with the plasmid antigen alone which could be measured at least 6 months after vaccination. Further, this translated to an improved outcome after challenge of long term rested mice that were previously immunized. The strength of the immune response as well as the number of Ag-specific T-cells is proportional to the number of Ag-specific cells primed by the vaccination regimen.",
keywords = "Adjuvant, IL-12, Vaccine",
author = "Chattergoon, {Michael Anand} and Vera Saulino and Shames, {Jason P.} and Jamie Stein and Montaner, {Luis J.} and Weiner, {David B.}",
year = "2004",
month = "4",
day = "16",
doi = "10.1016/j.vaccine.2004.01.036",
language = "English (US)",
volume = "22",
pages = "1744--1750",
journal = "Vaccine",
issn = "0264-410X",
publisher = "Elsevier BV",
number = "13-14",

}

TY - JOUR

T1 - Co-immunization with plasmid IL-12 generates a strong T-cell memory response in mice

AU - Chattergoon, Michael Anand

AU - Saulino, Vera

AU - Shames, Jason P.

AU - Stein, Jamie

AU - Montaner, Luis J.

AU - Weiner, David B.

PY - 2004/4/16

Y1 - 2004/4/16

N2 - Plasmid encoded exogenous IL-12 delivered as a DNA vaccine adjuvant has been shown to improve vaccine-induced immunity. In particular, pIL-12 greatly improves antigen (Ag)-specific cytotoxic tlymphocyte (CTL) activity in immunized mice. The longevity of this response has not previously been studied in detail. We have studied the effect of co-immunization with pIL-12 on HIV gp160 and Influenza A Hemeagglutinnin-specific memory immune responses. Mice co-immunized with pIL-12 and plasmid encoded antigens maintained a greater memory response than those immunized with the plasmid antigen alone which could be measured at least 6 months after vaccination. Further, this translated to an improved outcome after challenge of long term rested mice that were previously immunized. The strength of the immune response as well as the number of Ag-specific T-cells is proportional to the number of Ag-specific cells primed by the vaccination regimen.

AB - Plasmid encoded exogenous IL-12 delivered as a DNA vaccine adjuvant has been shown to improve vaccine-induced immunity. In particular, pIL-12 greatly improves antigen (Ag)-specific cytotoxic tlymphocyte (CTL) activity in immunized mice. The longevity of this response has not previously been studied in detail. We have studied the effect of co-immunization with pIL-12 on HIV gp160 and Influenza A Hemeagglutinnin-specific memory immune responses. Mice co-immunized with pIL-12 and plasmid encoded antigens maintained a greater memory response than those immunized with the plasmid antigen alone which could be measured at least 6 months after vaccination. Further, this translated to an improved outcome after challenge of long term rested mice that were previously immunized. The strength of the immune response as well as the number of Ag-specific T-cells is proportional to the number of Ag-specific cells primed by the vaccination regimen.

KW - Adjuvant

KW - IL-12

KW - Vaccine

UR - http://www.scopus.com/inward/record.url?scp=1842584373&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1842584373&partnerID=8YFLogxK

U2 - 10.1016/j.vaccine.2004.01.036

DO - 10.1016/j.vaccine.2004.01.036

M3 - Article

C2 - 15068858

AN - SCOPUS:1842584373

VL - 22

SP - 1744

EP - 1750

JO - Vaccine

JF - Vaccine

SN - 0264-410X

IS - 13-14

ER -