Co-immunization with plasmid IL-12 generates a strong T-cell memory response in mice

Michael A. Chattergoon, Vera Saulino, Jason P. Shames, Jamie Stein, Luis J. Montaner, David B. Weiner

Research output: Contribution to journalArticle

Abstract

Plasmid encoded exogenous IL-12 delivered as a DNA vaccine adjuvant has been shown to improve vaccine-induced immunity. In particular, pIL-12 greatly improves antigen (Ag)-specific cytotoxic tlymphocyte (CTL) activity in immunized mice. The longevity of this response has not previously been studied in detail. We have studied the effect of co-immunization with pIL-12 on HIV gp160 and Influenza A Hemeagglutinnin-specific memory immune responses. Mice co-immunized with pIL-12 and plasmid encoded antigens maintained a greater memory response than those immunized with the plasmid antigen alone which could be measured at least 6 months after vaccination. Further, this translated to an improved outcome after challenge of long term rested mice that were previously immunized. The strength of the immune response as well as the number of Ag-specific T-cells is proportional to the number of Ag-specific cells primed by the vaccination regimen.

Original languageEnglish (US)
Pages (from-to)1744-1750
Number of pages7
JournalVaccine
Volume22
Issue number13-14
DOIs
StatePublished - Apr 16 2004
Externally publishedYes

Keywords

  • Adjuvant
  • IL-12
  • Vaccine

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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  • Cite this

    Chattergoon, M. A., Saulino, V., Shames, J. P., Stein, J., Montaner, L. J., & Weiner, D. B. (2004). Co-immunization with plasmid IL-12 generates a strong T-cell memory response in mice. Vaccine, 22(13-14), 1744-1750. https://doi.org/10.1016/j.vaccine.2004.01.036