Co-expression of endothelial and neuronal nitric oxide synthases in the developing vasculatures of the human fetal eye

D. Scott McLeod, Takayuki Baba, Imran A. Bhutto, Gerard Anthony Lutty

Research output: Contribution to journalArticle

Abstract

Background: Nitric oxide (NO) is a multifunctional gaseous molecule that regulates various physiological functions in both neuronal and non-neuronal cells. NO is synthesized by nitric oxide synthases (NOSs), of which three isoforms have been identified. Neuronal NOS (nNOS) and endothelial NOS (eNOS) constitutively produce low levels of NO as a cellsignaling molecule in response to an increase in intracellular calcium concentration. Recent data have revealed a predominant role of eNOS in both angiogenesis and vasculogenesis. Methods: The immunohistochemical localization of nNOS and eNOS was investigated during embryonic and fetal ocular vascular development from 7 to 21 weeks gestation (WG) on sections of cryopreserved tissue. Results: eNOS was confined to endothelial cells of developing vessels at all ages studied. nNOS was prominent in nuclei of vascular endothelial and smooth muscle cells in the fetal vasculature of vitreous and choriocapillaris. nNOS was also prominent in the nuclei of CXCR4 + progenitors in the inner retina and inner neuroblastic layer. Conclusions: These findings demonstrate co-expression of n- and eNOS isoforms in different compartments of vasoformative cells during development. Nuclear nNOS was present in vascular and nonvascular progenitors as well as endothelial cells and pericytes. This suggests that nNOS may play a role in the transcription regulatory systems in endothelial cells and pericytes during ocular hemo-vasculogenesis, vasculogenesis, and angiogenesis.

Original languageEnglish (US)
Pages (from-to)839-848
Number of pages10
JournalGraefe's Archive for Clinical and Experimental Ophthalmology
Volume250
Issue number6
DOIs
StatePublished - Jun 2012

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Nitric Oxide Synthase Type I
Nitric Oxide Synthase Type III
Pericytes
Nitric Oxide
Endothelial Cells
Blood Vessels
Protein Isoforms
Vascular Smooth Muscle
Nitric Oxide Synthase
Smooth Muscle Myocytes
Retina
Stem Cells
Calcium
Pregnancy

Keywords

  • Choroidal vasculature
  • Fetal vasculature of vitreous
  • Nitric oxide synthase
  • Nucleus
  • Progenitors
  • Retinal vasculature

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

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title = "Co-expression of endothelial and neuronal nitric oxide synthases in the developing vasculatures of the human fetal eye",
abstract = "Background: Nitric oxide (NO) is a multifunctional gaseous molecule that regulates various physiological functions in both neuronal and non-neuronal cells. NO is synthesized by nitric oxide synthases (NOSs), of which three isoforms have been identified. Neuronal NOS (nNOS) and endothelial NOS (eNOS) constitutively produce low levels of NO as a cellsignaling molecule in response to an increase in intracellular calcium concentration. Recent data have revealed a predominant role of eNOS in both angiogenesis and vasculogenesis. Methods: The immunohistochemical localization of nNOS and eNOS was investigated during embryonic and fetal ocular vascular development from 7 to 21 weeks gestation (WG) on sections of cryopreserved tissue. Results: eNOS was confined to endothelial cells of developing vessels at all ages studied. nNOS was prominent in nuclei of vascular endothelial and smooth muscle cells in the fetal vasculature of vitreous and choriocapillaris. nNOS was also prominent in the nuclei of CXCR4 + progenitors in the inner retina and inner neuroblastic layer. Conclusions: These findings demonstrate co-expression of n- and eNOS isoforms in different compartments of vasoformative cells during development. Nuclear nNOS was present in vascular and nonvascular progenitors as well as endothelial cells and pericytes. This suggests that nNOS may play a role in the transcription regulatory systems in endothelial cells and pericytes during ocular hemo-vasculogenesis, vasculogenesis, and angiogenesis.",
keywords = "Choroidal vasculature, Fetal vasculature of vitreous, Nitric oxide synthase, Nucleus, Progenitors, Retinal vasculature",
author = "{Scott McLeod}, D. and Takayuki Baba and Bhutto, {Imran A.} and Lutty, {Gerard Anthony}",
year = "2012",
month = "6",
doi = "10.1007/s00417-012-1969-9",
language = "English (US)",
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pages = "839--848",
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issn = "0721-832X",
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number = "6",

}

TY - JOUR

T1 - Co-expression of endothelial and neuronal nitric oxide synthases in the developing vasculatures of the human fetal eye

AU - Scott McLeod, D.

AU - Baba, Takayuki

AU - Bhutto, Imran A.

AU - Lutty, Gerard Anthony

PY - 2012/6

Y1 - 2012/6

N2 - Background: Nitric oxide (NO) is a multifunctional gaseous molecule that regulates various physiological functions in both neuronal and non-neuronal cells. NO is synthesized by nitric oxide synthases (NOSs), of which three isoforms have been identified. Neuronal NOS (nNOS) and endothelial NOS (eNOS) constitutively produce low levels of NO as a cellsignaling molecule in response to an increase in intracellular calcium concentration. Recent data have revealed a predominant role of eNOS in both angiogenesis and vasculogenesis. Methods: The immunohistochemical localization of nNOS and eNOS was investigated during embryonic and fetal ocular vascular development from 7 to 21 weeks gestation (WG) on sections of cryopreserved tissue. Results: eNOS was confined to endothelial cells of developing vessels at all ages studied. nNOS was prominent in nuclei of vascular endothelial and smooth muscle cells in the fetal vasculature of vitreous and choriocapillaris. nNOS was also prominent in the nuclei of CXCR4 + progenitors in the inner retina and inner neuroblastic layer. Conclusions: These findings demonstrate co-expression of n- and eNOS isoforms in different compartments of vasoformative cells during development. Nuclear nNOS was present in vascular and nonvascular progenitors as well as endothelial cells and pericytes. This suggests that nNOS may play a role in the transcription regulatory systems in endothelial cells and pericytes during ocular hemo-vasculogenesis, vasculogenesis, and angiogenesis.

AB - Background: Nitric oxide (NO) is a multifunctional gaseous molecule that regulates various physiological functions in both neuronal and non-neuronal cells. NO is synthesized by nitric oxide synthases (NOSs), of which three isoforms have been identified. Neuronal NOS (nNOS) and endothelial NOS (eNOS) constitutively produce low levels of NO as a cellsignaling molecule in response to an increase in intracellular calcium concentration. Recent data have revealed a predominant role of eNOS in both angiogenesis and vasculogenesis. Methods: The immunohistochemical localization of nNOS and eNOS was investigated during embryonic and fetal ocular vascular development from 7 to 21 weeks gestation (WG) on sections of cryopreserved tissue. Results: eNOS was confined to endothelial cells of developing vessels at all ages studied. nNOS was prominent in nuclei of vascular endothelial and smooth muscle cells in the fetal vasculature of vitreous and choriocapillaris. nNOS was also prominent in the nuclei of CXCR4 + progenitors in the inner retina and inner neuroblastic layer. Conclusions: These findings demonstrate co-expression of n- and eNOS isoforms in different compartments of vasoformative cells during development. Nuclear nNOS was present in vascular and nonvascular progenitors as well as endothelial cells and pericytes. This suggests that nNOS may play a role in the transcription regulatory systems in endothelial cells and pericytes during ocular hemo-vasculogenesis, vasculogenesis, and angiogenesis.

KW - Choroidal vasculature

KW - Fetal vasculature of vitreous

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KW - Nucleus

KW - Progenitors

KW - Retinal vasculature

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