Niemann-Pick disease type C (NPC) is a neurovisceral storage disorder with an unknown primary deficiency. Somatic cell hybridization experiments using human cultured fibroblasts have shown that two complementation groups (NPC-α and NPC-β) are associated with the biochemical and clinical phenotypes comprising NPC. We identified the rarer complementation group NPC-β originally using the technique of filipin staining as a marker for complementation. In this study we show that the esterification of cholesterol derived from the LDL pathway can be used as an isotopic assay. However, multinuclear hybrids exhibit a delayed induction in this pathway. Furthermore, we discovered that, in the presence of an LDL source, co-cultivation of fibroblasts belonging to NPC-α and NPC-β stimulated cholesterol esterification.
|Original language||English (US)|
|Number of pages||6|
|Journal||Journal of Inherited Metabolic Disease|
|Publication status||Published - 1996|
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