Co-circulation of multiple species of Rickettsiales bacteria in one single species of hard ticks in Shenyang, China

Xue Dong, Xiao Ping Chen, Na Liu, Stephen J. Dumler, Yong Zhen Zhang

Research output: Contribution to journalArticle

Abstract

Bacteria in the order Rickettsiales include some of the most important zoonotic (re)emerging pathogens for animals and humans. In 2012, a total of 1267 adult Haemaphysalis longicornis ticks were collected from domestic animals (cattle and sheep) in Shenyang of Liaoning Province, China. These ticks were grouped into 181 pools (each pool with 6-7 ticks). Rickettsiales agents were identified in 93 (51.38%) tick pools using PCR targeting rrs (16S rRNA). In addition to 16S rRNA gene sequences, gltA and groEL gene sequences were also recovered from these positive samples. Phylogenetic analyses of these sequences revealed the presence of five species of Rickettsiales bacteria in a single tick species (. H. longicornis) in nature in Shenyang, including Rickettsia japonica, Rickettsia raoultii, Anaplasma centrale, Anaplasma bovis, and a potentially novel A. phagocytophilum variant. Additionally, two Candidatus Ehrlichia spp. (. Ehrlichia sp. Yonaguni138, Candidatus Ehrlichia shimanensis) were also identified in these ticks, with the highest prevalence of Ehrlichia sp. Yonaguni138 (73/181, 40.3%). Notably, these agents except the novel A. phagocytophilum variant had close evolutionary relationships with those previously identified in northeastern Asian countries including Korea, Japan, and Russia, indicating a geographic clustering pattern. Our data also reinforce the need for vigilance in recognition and prevention of rickettsiosis, ehrlichiosis, and anaplasmosis in humans and animals in this region.

Original languageEnglish (US)
Pages (from-to)727-733
Number of pages7
JournalTicks and Tick-borne Diseases
Volume5
Issue number6
DOIs
StatePublished - Oct 1 2014
Externally publishedYes

Fingerprint

Rickettsiales
Ixodidae
Ticks
Ehrlichia
ticks
China
Bacteria
bacteria
Haemaphysalis longicornis
Rickettsia
Anaplasma centrale
Rickettsia japonica
Anaplasma bovis
Anaplasma
Anaplasmosis
ribosomal RNA
Domestic Sheep
Ehrlichiosis
nucleotide sequences
animal pathogens

Keywords

  • Anaplasma
  • Ehrlichia
  • Phylogenetic analyses
  • Rickettsia
  • Ticks

ASJC Scopus subject areas

  • Infectious Diseases
  • Insect Science
  • Parasitology
  • Microbiology

Cite this

Co-circulation of multiple species of Rickettsiales bacteria in one single species of hard ticks in Shenyang, China. / Dong, Xue; Chen, Xiao Ping; Liu, Na; Dumler, Stephen J.; Zhang, Yong Zhen.

In: Ticks and Tick-borne Diseases, Vol. 5, No. 6, 01.10.2014, p. 727-733.

Research output: Contribution to journalArticle

Dong, Xue ; Chen, Xiao Ping ; Liu, Na ; Dumler, Stephen J. ; Zhang, Yong Zhen. / Co-circulation of multiple species of Rickettsiales bacteria in one single species of hard ticks in Shenyang, China. In: Ticks and Tick-borne Diseases. 2014 ; Vol. 5, No. 6. pp. 727-733.
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