Co-circulating microorganisms in questing Ixodes scapularis nymphs in Maryland

Katherine I. Swanson, Douglas Norris

Research output: Contribution to journalArticle

Abstract

Ixodes scapularis can be infected with Borrelia burgdorferi, Anaplasma phagocytophilum, Bartonella spp., Babesia microti, and Rickettsia spp., including spotted-fever group Rickettsia. As all of these microorganisms have been reported in Maryland, the potential for these ticks to have concurrent infections exists in this region. To assess the frequency of these complex infections, 348 I. scapularis nymphs collected in 2003 were screened for these microorganisms by PCR with positives being confirmed by DNA sequencing. Borrelia burgdorferi was detected in 14.7% of nymphs. Anaplasma phagocytophilum (0.3%), Rickettsia spp. (19.5%), and an uncategorized agent (0.9%) was also detected. Dual infections were detected with B. burgdorferi and Rickettsia spp. as well as a triple infection with B. burgdorferi, Rickettsia spp., and an uncategorized agent. Infections with B. burgdorferi and Rickettsia spp. were statistically independent of one another. However, infection with B. burgdorferi and any one of these other microorganisms appears to occur more frequently than by chance alone, probably as a result of shared enzootic cycles. This study confirms that multiple microorganisms co-circulate with B. burgdorferi in I. scapularis in Maryland and demonstrates that Rickettsia spp. and B. burgdorferi circulate independently and at nearly equal frequencies, while A. phagocytophilum and other unrecognized organisms are less common.

Original languageEnglish (US)
Pages (from-to)243-251
Number of pages9
JournalJournal of Vector Ecology
Volume32
Issue number2
DOIs
StatePublished - Dec 2007

Fingerprint

Ixodes scapularis
Borrelia burgdorferi
Rickettsia
nymphs
microorganism
microorganisms
Anaplasma phagocytophilum
infection
tick
Babesia microti
Bartonella
mixed infection
fever
ticks
DNA
sequence analysis
organisms

Keywords

  • Anaplasma phagocytophilum
  • Borrelia burgdorferi
  • Co-circulating
  • Ixodes scapularis
  • Rickettsia spp.

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Insect Science

Cite this

Co-circulating microorganisms in questing Ixodes scapularis nymphs in Maryland. / Swanson, Katherine I.; Norris, Douglas.

In: Journal of Vector Ecology, Vol. 32, No. 2, 12.2007, p. 243-251.

Research output: Contribution to journalArticle

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abstract = "Ixodes scapularis can be infected with Borrelia burgdorferi, Anaplasma phagocytophilum, Bartonella spp., Babesia microti, and Rickettsia spp., including spotted-fever group Rickettsia. As all of these microorganisms have been reported in Maryland, the potential for these ticks to have concurrent infections exists in this region. To assess the frequency of these complex infections, 348 I. scapularis nymphs collected in 2003 were screened for these microorganisms by PCR with positives being confirmed by DNA sequencing. Borrelia burgdorferi was detected in 14.7{\%} of nymphs. Anaplasma phagocytophilum (0.3{\%}), Rickettsia spp. (19.5{\%}), and an uncategorized agent (0.9{\%}) was also detected. Dual infections were detected with B. burgdorferi and Rickettsia spp. as well as a triple infection with B. burgdorferi, Rickettsia spp., and an uncategorized agent. Infections with B. burgdorferi and Rickettsia spp. were statistically independent of one another. However, infection with B. burgdorferi and any one of these other microorganisms appears to occur more frequently than by chance alone, probably as a result of shared enzootic cycles. This study confirms that multiple microorganisms co-circulate with B. burgdorferi in I. scapularis in Maryland and demonstrates that Rickettsia spp. and B. burgdorferi circulate independently and at nearly equal frequencies, while A. phagocytophilum and other unrecognized organisms are less common.",
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