Clustered α-amanitin resistance mutations in mouse

Marisa S. Bartolomei, Jeffry Lynn Corden

Research output: Contribution to journalArticle

Abstract

We report the identification of three new α-amanitin resistance mutations in the gene encoding the largest subunit of mouse RNA polymerase II (RPII215). These mutations are clustered in a region of the largest subunit that is important for transcription elongation. This same domain has been identified as the site of α-amanitin resistance mutations in both Drosophila and Caenarhabditis elegans. The sequences encompassing this cluster of mutations are highly conserved among RNA polymerase II genes from a number of species, including those that are naturally more resistant to α-amanitin suggesting that this region of the largest subunit is critical for a conserved catalytic function. The mutations reported here change leucine 745 to phenylalanine, arginine 749 to proline, or isoleucine 779 to phenylalanine. Together with the previously reported asparagine 792 to aspartate substitution these mutations define a potential α-amanitin binding pocket in a region of the mouse subunit that could be involved in translocation of polymerase during elongation.

Original languageEnglish (US)
Pages (from-to)778-782
Number of pages5
JournalMGG Molecular & General Genetics
Volume246
Issue number6
DOIs
StatePublished - Nov 1995

Fingerprint

Amanitins
Mutation
Phenylalanine
RNA Polymerase II
Isoleucine
Asparagine
Proline
Aspartic Acid
Leucine
Genes
Drosophila
Arginine

Keywords

  • α-Amanitin Mutation
  • RNA polymerase II
  • Transcription

ASJC Scopus subject areas

  • Genetics

Cite this

Clustered α-amanitin resistance mutations in mouse. / Bartolomei, Marisa S.; Corden, Jeffry Lynn.

In: MGG Molecular & General Genetics, Vol. 246, No. 6, 11.1995, p. 778-782.

Research output: Contribution to journalArticle

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