Closing and characterization of cDNAs coding for heavy and light chains of a monoclonal antibody (MabA34) specific for human plasma apolipoprotein A-I

Ju Won Kwak, Dong Lee, Byung Kwon Choi, Won Kyung Cho, Sang Han Lee, Yong Bok Park, Moon Hi Han

Research output: Contribution to journalArticle

Abstract

We have determined the nucleotide (nt) sequences encoding the heavy (H)- and light (L)-chains of the Fab fragment of a murine monoclonal antibody, MabA34 (γl, κ), which is specific for human plasma apolipoprotein A-I of high-density lipoproteins. The variable (V) regions of the H- and L-chains were revealed to be members of mouse I-I-chain subgroup II(A) and κ L-chain subgroup II, respectively. A few unusual amino acids in the V region of the H-chain, and nt residues probably introduced by somatic mutations from germline genes were also identified.

Original languageEnglish (US)
Pages (from-to)257-259
Number of pages3
JournalGene
Volume173
Issue number2
DOIs
Publication statusPublished - Sep 16 1996
Externally publishedYes

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Keywords

  • Apo A-I
  • DNA sequencing
  • HDL cholesterol
  • High-density lipoproteins
  • Immunoglobulin Fab
  • Polymerase chain reaction
  • Recombinant DNA

ASJC Scopus subject areas

  • Genetics

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