Closing and characterization of cDNAs coding for heavy and light chains of a monoclonal antibody (MabA34) specific for human plasma apolipoprotein A-I

Ju Won Kwak; Dong Ik Lee; Byung Kwon Choi; Won Kyung Cho; Sang Han Lee; Yong Bok Park; Moon Hi Han

doi:10.1016/0378-1119(96)00077-7

Closing and characterization of cDNAs coding for heavy and light chains of a monoclonal antibody (MabA34) specific for human plasma apolipoprotein A-I

Ju Won Kwak, Dong Ik Lee, Byung Kwon Choi, Won Kyung Cho, Sang Han Lee, Yong Bok Park, Moon Hi Han

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

We have determined the nucleotide (nt) sequences encoding the heavy (H)- and light (L)-chains of the Fab fragment of a murine monoclonal antibody, MabA34 (γl, κ), which is specific for human plasma apolipoprotein A-I of high-density lipoproteins. The variable (V) regions of the H- and L-chains were revealed to be members of mouse I-I-chain subgroup II(A) and κ L-chain subgroup II, respectively. A few unusual amino acids in the V region of the H-chain, and nt residues probably introduced by somatic mutations from germline genes were also identified.

Original language	English (US)
Pages (from-to)	257-259
Number of pages	3
Journal	Gene
Volume	173
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(96)00077-7
State	Published - Sep 16 1996
Externally published	Yes

Keywords

Apo A-I
DNA sequencing
HDL cholesterol
High-density lipoproteins
Immunoglobulin Fab
Polymerase chain reaction
Recombinant DNA

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(96)00077-7

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title = "Closing and characterization of cDNAs coding for heavy and light chains of a monoclonal antibody (MabA34) specific for human plasma apolipoprotein A-I",

abstract = "We have determined the nucleotide (nt) sequences encoding the heavy (H)- and light (L)-chains of the Fab fragment of a murine monoclonal antibody, MabA34 (γl, κ), which is specific for human plasma apolipoprotein A-I of high-density lipoproteins. The variable (V) regions of the H- and L-chains were revealed to be members of mouse I-I-chain subgroup II(A) and κ L-chain subgroup II, respectively. A few unusual amino acids in the V region of the H-chain, and nt residues probably introduced by somatic mutations from germline genes were also identified.",

keywords = "Apo A-I, DNA sequencing, HDL cholesterol, High-density lipoproteins, Immunoglobulin Fab, Polymerase chain reaction, Recombinant DNA",

author = "Kwak, {Ju Won} and Lee, {Dong Ik} and Choi, {Byung Kwon} and Cho, {Won Kyung} and Lee, {Sang Han} and Park, {Yong Bok} and Han, {Moon Hi}",

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doi = "10.1016/0378-1119(96)00077-7",

language = "English (US)",

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pages = "257--259",

journal = "Gene",

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publisher = "Elsevier",

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T1 - Closing and characterization of cDNAs coding for heavy and light chains of a monoclonal antibody (MabA34) specific for human plasma apolipoprotein A-I

AU - Kwak, Ju Won

AU - Lee, Dong Ik

AU - Choi, Byung Kwon

AU - Cho, Won Kyung

AU - Lee, Sang Han

AU - Park, Yong Bok

AU - Han, Moon Hi

PY - 1996/9/16

Y1 - 1996/9/16

N2 - We have determined the nucleotide (nt) sequences encoding the heavy (H)- and light (L)-chains of the Fab fragment of a murine monoclonal antibody, MabA34 (γl, κ), which is specific for human plasma apolipoprotein A-I of high-density lipoproteins. The variable (V) regions of the H- and L-chains were revealed to be members of mouse I-I-chain subgroup II(A) and κ L-chain subgroup II, respectively. A few unusual amino acids in the V region of the H-chain, and nt residues probably introduced by somatic mutations from germline genes were also identified.

AB - We have determined the nucleotide (nt) sequences encoding the heavy (H)- and light (L)-chains of the Fab fragment of a murine monoclonal antibody, MabA34 (γl, κ), which is specific for human plasma apolipoprotein A-I of high-density lipoproteins. The variable (V) regions of the H- and L-chains were revealed to be members of mouse I-I-chain subgroup II(A) and κ L-chain subgroup II, respectively. A few unusual amino acids in the V region of the H-chain, and nt residues probably introduced by somatic mutations from germline genes were also identified.

KW - Apo A-I

KW - DNA sequencing

KW - HDL cholesterol

KW - High-density lipoproteins

KW - Immunoglobulin Fab

KW - Polymerase chain reaction

KW - Recombinant DNA

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Closing and characterization of cDNAs coding for heavy and light chains of a monoclonal antibody (MabA34) specific for human plasma apolipoprotein A-I

Abstract

Keywords

ASJC Scopus subject areas

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