Close association of invading Plasmodium berghei and β integrin in the Anopheles gambiae midgut

Vasso Mahairaki, Gareth Lycett, Inga Sidén-Kiamos, Robert E. Sinden, Christos Louis

Research output: Contribution to journalArticlepeer-review

Abstract

We have used confocal microscopy and an antibody against Anopheles gambiae β integrin to study this protein's distribution in the mosquito midgut and its relationship to invading Plasmodium berghei parasites. An extensive reorganization of integrin is seen to take place in the midgut epithelial cells following the uptake of either non-infected or parasite-infected blood meal, probably reflecting the reshaping of the gut due to the presence of the food bolus and the peritrophic membrane that surrounds it. Furthermore, malaria parasites are coated with β integrin immediately upon entry into the epithelium, independent of whether they develop intra- or extracellularly. Although this coat is shed a few days after the invasion, β integrin remains concentrated in the cells surrounding the maturing oocyst for several days. Finally, the antibody detects a structural change in the midgut epithelial cells in the immediate vicinity of the invading ookinete, which is consistent with Plasmodium-induced apoptosis followed by wound healing. This intimate association suggests a specific role of β integrin in the invasion process.

Original languageEnglish (US)
Pages (from-to)13-19
Number of pages7
JournalArchives of Insect Biochemistry and Physiology
Volume60
Issue number1
DOIs
StatePublished - Sep 1 2005
Externally publishedYes

Keywords

  • Anopheles
  • Apoptosis
  • Basal lamina
  • Integrin
  • Laminin
  • Malaria
  • Wound healing

ASJC Scopus subject areas

  • Physiology
  • Biochemistry
  • Insect Science

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