Abstract
We have used confocal microscopy and an antibody against Anopheles gambiae β integrin to study this protein's distribution in the mosquito midgut and its relationship to invading Plasmodium berghei parasites. An extensive reorganization of integrin is seen to take place in the midgut epithelial cells following the uptake of either non-infected or parasite-infected blood meal, probably reflecting the reshaping of the gut due to the presence of the food bolus and the peritrophic membrane that surrounds it. Furthermore, malaria parasites are coated with β integrin immediately upon entry into the epithelium, independent of whether they develop intra- or extracellularly. Although this coat is shed a few days after the invasion, β integrin remains concentrated in the cells surrounding the maturing oocyst for several days. Finally, the antibody detects a structural change in the midgut epithelial cells in the immediate vicinity of the invading ookinete, which is consistent with Plasmodium-induced apoptosis followed by wound healing. This intimate association suggests a specific role of β integrin in the invasion process.
Original language | English (US) |
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Pages (from-to) | 13-19 |
Number of pages | 7 |
Journal | Archives of Insect Biochemistry and Physiology |
Volume | 60 |
Issue number | 1 |
DOIs | |
State | Published - Sep 1 2005 |
Externally published | Yes |
Keywords
- Anopheles
- Apoptosis
- Basal lamina
- Integrin
- Laminin
- Malaria
- Wound healing
ASJC Scopus subject areas
- Physiology
- Biochemistry
- Insect Science