Cloning, sequence analysis, and processing of the rat and human atrial natriuretic peptide precursors

J. A. Lewicki, B. Greenberg, M. Yamanaka, G. Vlasuk, M. Brewer, D. Gardner, J. Baxter, L. K. Johnson, J. C. Fiddes

Research output: Contribution to journalReview articlepeer-review

29 Scopus citations


Complementary DNA sequences and structural genes encoding the atrial natriuretic peptide precursor (prepro-ANP) have been cloned. Analysis of DNA sequences, complementary to rat atrial prepro-ANP mRNA, has revealed that the various natriuretic peptides isolated from rat atrium reside at the carboxy terminus of a 152-amino-acid precursor protein. The human gene, comprised of three exons and two intervening sequences, encodes a protein of 151 amino acids highly homologous to the rat precursors. Although putative proteolytic processing sites can be identified throughout the prepro-ANP amino acid sequence, the natural form of the mature ANP has not been identified. Therefore, the sites and mechanisms of prepro-ANP processing to mature peptides forms are unknown. However, the successful cloning of the prepro-ANP gene and corresponding cDNAs provide the necessary molecular tools to address these fundamental questions relating to the regulation of ANP synthesis and processing in atrial and extraatrial tissues.

Original languageEnglish (US)
Pages (from-to)2086-2090
Number of pages5
JournalFederation Proceedings
Issue number7
StatePublished - Sep 24 1986
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)


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