Cloning of the gene coding for a shared human melanoma antigen recognized by autologous T cells infiltrating into tumor

Yutaka Kawakami, Siona Eliyahu, Cynthia H. Delgado, Paul F. Robbins, Licia Rivoltini, Suzanne Topalian, Toru Miki, Steven A. Rosenberg

Research output: Contribution to journalArticle

Abstract

By cDNA expression cloning we have isolated a gene encoding a shared human melanoma antigen recognized by HLA-A2 restricted autologous and allogeneic tumor-infiltrating lymphocytes (TILs) from patients with metastatic melanoma. By using both transient and stable expression systems, transfection of this gene into non-antigen-expressing HLA-A2+ cell lines resulted in recognition by the antigen-specific TILs. The sequence of this cDNA revealed a previously undescribed putative transmembrane protein whose expression was restricted to melanoma and melanocyte cell lines and human retina but no other fresh or cultured normal tissues tested or other tumor histologies. Thus, we have identified a gene encoding a melanocyte lineage-specific protein (MART-1; melanoma antigen recognized by T cells 1) that is a widely shared melanoma antigen recognized by the T lymphocytes of patients with established malignancy. Identification of this gene opens possibilities for the development of immunotherapies for patients with melanoma.

Original languageEnglish (US)
Pages (from-to)3515-3519
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume91
Issue number9
StatePublished - Apr 26 1994
Externally publishedYes

Fingerprint

Melanoma-Specific Antigens
Organism Cloning
MART-1 Antigen
T-Lymphocytes
Tumor-Infiltrating Lymphocytes
HLA-A2 Antigen
Melanoma
Melanocytes
Genes
Neoplasms
Complementary DNA
Cell Line
Immunotherapy
Transfection
Retina
Histology
Proteins
Antigens

Keywords

  • HLA-A2
  • immunotherapy
  • MART-1
  • melanocyte
  • tumor antigen

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Cloning of the gene coding for a shared human melanoma antigen recognized by autologous T cells infiltrating into tumor. / Kawakami, Yutaka; Eliyahu, Siona; Delgado, Cynthia H.; Robbins, Paul F.; Rivoltini, Licia; Topalian, Suzanne; Miki, Toru; Rosenberg, Steven A.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, No. 9, 26.04.1994, p. 3515-3519.

Research output: Contribution to journalArticle

Kawakami, Yutaka ; Eliyahu, Siona ; Delgado, Cynthia H. ; Robbins, Paul F. ; Rivoltini, Licia ; Topalian, Suzanne ; Miki, Toru ; Rosenberg, Steven A. / Cloning of the gene coding for a shared human melanoma antigen recognized by autologous T cells infiltrating into tumor. In: Proceedings of the National Academy of Sciences of the United States of America. 1994 ; Vol. 91, No. 9. pp. 3515-3519.
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