Cloning of the cDNA encoding human histidine decarboxylase from an erythroleukemia cell line and mapping of the gene locus to chromosome 15

Cynthia Zahnow, Hua Fanc Yi, O. Wesley Mcbride, David R. Joseph

Research output: Contribution to journalArticle

Abstract

The biogenic amine histamine is an important modulator of numerous physiological processes, including neurotransmitt-ance, gastric acid secretion and smooth muscle tone. The biosynthesis of histamine is catalyzed by the enzyme, L-histi-dine decarboxylase (HDC). We have previously reported the cloning and sequence of the cDNA encoding rat HDC. Utilizing the rat HDC cDNA as probe the full-length cDNA encoding human HDC was identified and characterized. The encoded protein of 662 amino acid residues has a molecular weight of 74,148. Homology comparisons of the deduced amino acid sequence with rat HDC and dopa decarboxylases from three species have revealed highly related regions. These comparisons have identified domains of amino acid decarboxylases that are highly conserved and are likely important for enzyme-substrate interaction. A dissimilar region in human and rat HDC primary translated protein near the C-terminus would appear not to be important for catalysis and may be removed by proteolysis. This processing phenomenon could be in part responsible for regulation of HDC activity. The human HDC cDNA was also utilized to map the chromosomal location of the human HDC gene locus (HDQ. Analysis of human-rodent cell hybrids revealed that the HDC gene segregates with Chromosome 15. No restriction length polymorphisms in the human population were detected after cleavage of the DNAs with 12 restriction endonucleases.

Original languageEnglish (US)
Pages (from-to)395-400
Number of pages6
JournalMitochondrial DNA
Volume1
Issue number6
DOIs
StatePublished - 1991
Externally publishedYes

Fingerprint

Histidine Decarboxylase
Chromosomes, Human, Pair 15
Leukemia, Erythroblastic, Acute
Carboxy-Lyases
Chromosome Mapping
Cloning
Chromosomes
Organism Cloning
Complementary DNA
Genes
Cells
Cell Line
Rats
Amino Acids
Histamine
Dopa Decarboxylase
Proteolysis
Physiological Phenomena
Biogenic Amines
DNA Cleavage

Keywords

  • CDNA
  • Dopa decarboxylase
  • Histamine
  • Histidine decarboxylase
  • Human

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Biochemistry
  • Endocrinology

Cite this

Cloning of the cDNA encoding human histidine decarboxylase from an erythroleukemia cell line and mapping of the gene locus to chromosome 15. / Zahnow, Cynthia; Yi, Hua Fanc; Mcbride, O. Wesley; Joseph, David R.

In: Mitochondrial DNA, Vol. 1, No. 6, 1991, p. 395-400.

Research output: Contribution to journalArticle

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