Cloning of FRK, a novel human intracellular SRC-like tyrosine kinaseencoding gene

James Lee, Zack Z. Wang, Shiuh Ming Luoh, William I. Wood, David T. Scadden

Research output: Contribution to journalArticle

Abstract

We report the cloning of a novel tyrosine kinase (TyK)-encoding gene (TYK) from the human hepatoma cell line Hep3B. Using the polymerase chain reaction (PCR) and oligodeoxyribonucleotide primers based on conserved TYK motifs, a 180-bp fragment was cloned and used to obtain full-length cDNA clones of 2.9 kb, with an open reading frame of 505 amino acids (aa). Restricted expression was detected by Northern blotting or reverse-transcribed PCR in a broad range of cell lines. The predicted aa sequence contains characteristic TyK motifs without a transmembrane region, suggesting an intracellular localization. There was 49% aa sequence identity with human FYN product and 47% with human SRC product; however, several structural differences distinguish this clone from other SRC subfamily members. This clone, FYN-related feinase or FRK, is a novel member of the intracellular TYK gene family.

Original languageEnglish (US)
Pages (from-to)247-251
Number of pages5
JournalGene
Volume138
Issue number1-2
DOIs
Publication statusPublished - Jan 28 1994
Externally publishedYes

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Keywords

  • cell regulation
  • differentiation controls
  • oncogenes
  • proliferation controls
  • Signal transduction

ASJC Scopus subject areas

  • Genetics

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