Abstract
We report the cloning of a novel tyrosine kinase (TyK)-encoding gene (TYK) from the human hepatoma cell line Hep3B. Using the polymerase chain reaction (PCR) and oligodeoxyribonucleotide primers based on conserved TYK motifs, a 180-bp fragment was cloned and used to obtain full-length cDNA clones of 2.9 kb, with an open reading frame of 505 amino acids (aa). Restricted expression was detected by Northern blotting or reverse-transcribed PCR in a broad range of cell lines. The predicted aa sequence contains characteristic TyK motifs without a transmembrane region, suggesting an intracellular localization. There was 49% aa sequence identity with human FYN product and 47% with human SRC product; however, several structural differences distinguish this clone from other SRC subfamily members. This clone, FYN-related feinase or FRK, is a novel member of the intracellular TYK gene family.
Original language | English (US) |
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Pages (from-to) | 247-251 |
Number of pages | 5 |
Journal | Gene |
Volume | 138 |
Issue number | 1-2 |
DOIs | |
State | Published - Jan 28 1994 |
Externally published | Yes |
Keywords
- Signal transduction
- cell regulation
- differentiation controls
- oncogenes
- proliferation controls
ASJC Scopus subject areas
- Genetics