Cloning of an Alpha-TFEB fusion in renal tumors harboring the t(6;11)(p21;q13) chromosome translocation

Ian J. Davis, Bae Li Hsi, Jason D. Arroyo, Sara O. Vargas, Y. Albert Yeh, Gabriela Motyckova, Patricia Valencia, Antonio R. Perez-Atayde, Pedram Argani, Marc Ladanyi, Jonathan A. Fletcher, David E. Fisher

Research output: Contribution to journalArticle

Abstract

MITF, TFE3, TFEB, and TFEC comprise a transcription factor family (MiT) that regulates key developmental pathways in several cell lineages. Like MYC, MiT members are basic helix-loop-helix-leucine zipper transcription factors. MiT members share virtually perfect homology in their DNA binding domains and bind a common DNA motif. Translocations of TFE3 occur in specific subsets of human renal cell carcinomas and in alveolar soft part sarcomas. Although multiple translocation partners are fused to TFE3, each translocation product retains TFE3's basic helix-loop-helix leucine zipper. We have identified the genes fused by the chromosomal translocation t(6;11)(p21.1;q13), characteristic of another subset of renal neoplasms. In two primary tumors we found that Alpha, an intronless gene, rearranges with the first intron of TFEB, just upstream of TFEB's initiation ATG, preserving the entire TFEB coding sequence. Fluorescence in situ hybridization confirmed the involvement of both TFEB and Alpha in this translocation. Although the Alpha promoter drives expression of this fusion gene, the Alpha gene does not contribute to the ORF. Whereas TFE3 is typically fused to partner proteins in subsets of renal tumors, we found that wild-type, unfused TFE3 stimulates clonogenic growth in a cell-based assay, suggesting that dysregulated expression, rather than altered function of TFEB or TFE3 fusions, may confer neoplastic properties, a mechanism reminiscent of MYC activation by promoter substitution in Burkitt's lymphoma. Alpha-TFEB is thus identified as a fusion gene in a subset of pediatric renal neoplasms.

Original languageEnglish (US)
Pages (from-to)6051-6056
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number10
DOIs
StatePublished - May 13 2003

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Organism Cloning
Chromosomes
Kidney Neoplasms
Gene Fusion
Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
Alveolar Soft Part Sarcoma
Genes
Leucine Zippers
Neoplasms
Genetic Translocation
Nucleotide Motifs
Burkitt Lymphoma
Sexual Partners
Cell Lineage
Fluorescence In Situ Hybridization
Renal Cell Carcinoma
Introns
Open Reading Frames
Transcription Factors
Pediatrics

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Cloning of an Alpha-TFEB fusion in renal tumors harboring the t(6;11)(p21;q13) chromosome translocation. / Davis, Ian J.; Hsi, Bae Li; Arroyo, Jason D.; Vargas, Sara O.; Yeh, Y. Albert; Motyckova, Gabriela; Valencia, Patricia; Perez-Atayde, Antonio R.; Argani, Pedram; Ladanyi, Marc; Fletcher, Jonathan A.; Fisher, David E.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, No. 10, 13.05.2003, p. 6051-6056.

Research output: Contribution to journalArticle

Davis, IJ, Hsi, BL, Arroyo, JD, Vargas, SO, Yeh, YA, Motyckova, G, Valencia, P, Perez-Atayde, AR, Argani, P, Ladanyi, M, Fletcher, JA & Fisher, DE 2003, 'Cloning of an Alpha-TFEB fusion in renal tumors harboring the t(6;11)(p21;q13) chromosome translocation', Proceedings of the National Academy of Sciences of the United States of America, vol. 100, no. 10, pp. 6051-6056. https://doi.org/10.1073/pnas.0931430100
Davis, Ian J. ; Hsi, Bae Li ; Arroyo, Jason D. ; Vargas, Sara O. ; Yeh, Y. Albert ; Motyckova, Gabriela ; Valencia, Patricia ; Perez-Atayde, Antonio R. ; Argani, Pedram ; Ladanyi, Marc ; Fletcher, Jonathan A. ; Fisher, David E. / Cloning of an Alpha-TFEB fusion in renal tumors harboring the t(6;11)(p21;q13) chromosome translocation. In: Proceedings of the National Academy of Sciences of the United States of America. 2003 ; Vol. 100, No. 10. pp. 6051-6056.
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AU - Davis, Ian J.

AU - Hsi, Bae Li

AU - Arroyo, Jason D.

AU - Vargas, Sara O.

AU - Yeh, Y. Albert

AU - Motyckova, Gabriela

AU - Valencia, Patricia

AU - Perez-Atayde, Antonio R.

AU - Argani, Pedram

AU - Ladanyi, Marc

AU - Fletcher, Jonathan A.

AU - Fisher, David E.

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