Cloning of a receptor subunit required for signaling by thymic stromal lymphopoietin

Akhilesh Pandey, Katsutoshi Ozaki, Heinz Baumann, Steven D. Levin, Anne Puel, Andrew G. Farr, Steven F. Ziegler, Warren J. Leonard, Harvey F. Lodish

Research output: Contribution to journalArticle

Abstract

Signaling by type I cytokines involves the formation of receptor homodimers, heterodimers or higher order receptor oligomers. Here we report the cloning of a type I cytokine receptor subunit that is most closely related to the common cytokine receptor γ chain (γc). Binding and crosslinking experiments demonstrate that this protein is the receptor for a recently described interleukin 7 (IL-7)-like factor, thymic stromal lymphopoietin (TSLP). Binding of TSLP to the thymic stromal lymphopoietin receptor (TSLPR) is increased markedly in the presence of the IL-7 receptor α chain (IL-7Rα). IL-7Rα-expressing but not parental 32D cells proliferate in the presence of exogenous TSLP. Moreover, a combination of IL-7Rα and TSLPR is required for TSLP-dependent activation of a STAT5-dependent reporter construct. Thus it is shown that IL-7Rα is a component of both the IL-7 and TSLP receptors, which helps to explain why deletion of the gene that encodes IL-7Rα affects the lymphoid system more severely than deletion of the gene encoding IL-7 does. Cloning of TSLPR should facilitate an understanding of TSLP function and its signaling mechanism.

Original languageEnglish (US)
Pages (from-to)59-64
Number of pages6
JournalNature Immunology
Volume1
Issue number1
StatePublished - Jul 2000

ASJC Scopus subject areas

  • Immunology

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    Pandey, A., Ozaki, K., Baumann, H., Levin, S. D., Puel, A., Farr, A. G., Ziegler, S. F., Leonard, W. J., & Lodish, H. F. (2000). Cloning of a receptor subunit required for signaling by thymic stromal lymphopoietin. Nature Immunology, 1(1), 59-64.