Cloning of a cDNA encoding sheep calcitonin from a thyroid C-cell library

A. Cumaraswamy; M. Borges; H. Tamir; B. D. Nelkin

doi:10.1016/0378-1119(93)90379-H

Cloning of a cDNA encoding sheep calcitonin from a thyroid C-cell library

A. Cumaraswamy, M. Borges, H. Tamir, B. D. Nelkin

School of Medicine

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

A sheep thyroid C-cell cDNA library constructed in λZAPII was screened with a probe specific for the human calcitonin (CT)-encoding gene (CT). Plaque lifts from a total of 6 × 10⁴ recombinant plaques identified six overlapping cDNA clones, one of which was an 806-bp cDNA representing an almost full-length copy of the sheep CT mRNA. The nucleotide (nt) sequence consists of a 71-bp 5'-flanking sequence, a protein-coding region of 429 bp with a coding potential for a 143-amino-acid (aa) protein with a deduced M_r of 15888 and a 306-bp 3'-untranslated region. Northern blot analysis of sheep thyroid tissue revealed two major mRNAs with sizes of 0.9 and 0.65 kb. The sheep CT cDNA shows 71% similarity at the nt level to human CT and 58% identity at the predicted aa level.

Original language	English (US)
Pages (from-to)	269-273
Number of pages	5
Journal	Gene
Volume	126
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(93)90379-H
State	Published - Apr 30 1993

Keywords

MEN 2A syndrome
cDNA library
medullary thyroid carcinoma

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(93)90379-H

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title = "Cloning of a cDNA encoding sheep calcitonin from a thyroid C-cell library",

abstract = "A sheep thyroid C-cell cDNA library constructed in λZAPII was screened with a probe specific for the human calcitonin (CT)-encoding gene (CT). Plaque lifts from a total of 6 × 104 recombinant plaques identified six overlapping cDNA clones, one of which was an 806-bp cDNA representing an almost full-length copy of the sheep CT mRNA. The nucleotide (nt) sequence consists of a 71-bp 5'-flanking sequence, a protein-coding region of 429 bp with a coding potential for a 143-amino-acid (aa) protein with a deduced Mr of 15888 and a 306-bp 3'-untranslated region. Northern blot analysis of sheep thyroid tissue revealed two major mRNAs with sizes of 0.9 and 0.65 kb. The sheep CT cDNA shows 71% similarity at the nt level to human CT and 58% identity at the predicted aa level.",

keywords = "MEN 2A syndrome, cDNA library, medullary thyroid carcinoma",

author = "A. Cumaraswamy and M. Borges and H. Tamir and Nelkin, {B. D.}",

note = "Funding Information: We thank Dr. StephenB aylin for his enthusiastiicn ter-est, advice,a nd support throughout this project. This study was supported by a grant from the American Cancer Society (PDT-417) and National Institutes of Health (DK19743).",

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doi = "10.1016/0378-1119(93)90379-H",

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AU - Cumaraswamy, A.

AU - Borges, M.

AU - Tamir, H.

AU - Nelkin, B. D.

N1 - Funding Information: We thank Dr. StephenB aylin for his enthusiastiicn ter-est, advice,a nd support throughout this project. This study was supported by a grant from the American Cancer Society (PDT-417) and National Institutes of Health (DK19743).

PY - 1993/4/30

Y1 - 1993/4/30

N2 - A sheep thyroid C-cell cDNA library constructed in λZAPII was screened with a probe specific for the human calcitonin (CT)-encoding gene (CT). Plaque lifts from a total of 6 × 104 recombinant plaques identified six overlapping cDNA clones, one of which was an 806-bp cDNA representing an almost full-length copy of the sheep CT mRNA. The nucleotide (nt) sequence consists of a 71-bp 5'-flanking sequence, a protein-coding region of 429 bp with a coding potential for a 143-amino-acid (aa) protein with a deduced Mr of 15888 and a 306-bp 3'-untranslated region. Northern blot analysis of sheep thyroid tissue revealed two major mRNAs with sizes of 0.9 and 0.65 kb. The sheep CT cDNA shows 71% similarity at the nt level to human CT and 58% identity at the predicted aa level.

AB - A sheep thyroid C-cell cDNA library constructed in λZAPII was screened with a probe specific for the human calcitonin (CT)-encoding gene (CT). Plaque lifts from a total of 6 × 104 recombinant plaques identified six overlapping cDNA clones, one of which was an 806-bp cDNA representing an almost full-length copy of the sheep CT mRNA. The nucleotide (nt) sequence consists of a 71-bp 5'-flanking sequence, a protein-coding region of 429 bp with a coding potential for a 143-amino-acid (aa) protein with a deduced Mr of 15888 and a 306-bp 3'-untranslated region. Northern blot analysis of sheep thyroid tissue revealed two major mRNAs with sizes of 0.9 and 0.65 kb. The sheep CT cDNA shows 71% similarity at the nt level to human CT and 58% identity at the predicted aa level.

KW - MEN 2A syndrome

KW - cDNA library

KW - medullary thyroid carcinoma

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JO - Gene

JF - Gene

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ER -

Cloning of a cDNA encoding sheep calcitonin from a thyroid C-cell library

Abstract

Keywords

ASJC Scopus subject areas

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