Cloning of a cDNA encoding sheep calcitonin from a thyroid C-cell library

A. Cumaraswamy, M. Borges, H. Tamir, B. D. Nelkin

Research output: Contribution to journalArticle

Abstract

A sheep thyroid C-cell cDNA library constructed in λZAPII was screened with a probe specific for the human calcitonin (CT)-encoding gene (CT). Plaque lifts from a total of 6 × 104 recombinant plaques identified six overlapping cDNA clones, one of which was an 806-bp cDNA representing an almost full-length copy of the sheep CT mRNA. The nucleotide (nt) sequence consists of a 71-bp 5'-flanking sequence, a protein-coding region of 429 bp with a coding potential for a 143-amino-acid (aa) protein with a deduced Mr of 15888 and a 306-bp 3'-untranslated region. Northern blot analysis of sheep thyroid tissue revealed two major mRNAs with sizes of 0.9 and 0.65 kb. The sheep CT cDNA shows 71% similarity at the nt level to human CT and 58% identity at the predicted aa level.

Original languageEnglish (US)
Pages (from-to)269-273
Number of pages5
JournalGene
Volume126
Issue number2
DOIs
StatePublished - Apr 30 1993

Keywords

  • MEN 2A syndrome
  • cDNA library
  • medullary thyroid carcinoma

ASJC Scopus subject areas

  • Genetics

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