Cloning, molecular characterization, and expression analysis of Copine 8

Rangan Maitra, Dmitry N. Grigoryev, Tapan Kumar Bera, Ira H. Pastan, Byungkook Lee

Research output: Contribution to journalArticlepeer-review

Abstract

Copines are ubiquitously expressed, phospholipid-binding proteins that have been conserved through evolution. In this paper, we report the cloning and molecular characterization of a new member of the Copine family, Copine 8. This gene has been isolated and characterized using a combination of bioinformatic and experimental approaches. Using an algorithm to cluster ESTs (expressed sequence tags) that are available through the public "GoldenPath" database, Copine 8 was initially identified as a gene predominantly expressed in prostate and testis. Cloning and molecular analysis revealed that this gene is expressed in low-levels in most tissues examined. Two different isoforms of this gene have been isolated. Strongest expression of Copine 8 mRNA is seen in the prostate, heart, and brain. Taken together, this data suggest that Copine 8 may have an important role to play in prostate regulation and development.

Original languageEnglish (US)
Pages (from-to)842-847
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume303
Issue number3
DOIs
StatePublished - Apr 11 2003

Keywords

  • Cloning
  • Copine
  • Expression analysis
  • Functional genomics
  • Isoforms
  • Prostate

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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