Cloning genes encoding MHC class II-restricted antigens: Mutated CDC27 as a tumor antigen

Rong Fu Wang, Xiang Wang, Alicia C. Atwood, Suzanne Topalian, Steven A. Rosenberg

Research output: Contribution to journalArticle

Abstract

In an effort to identify tumor-specific antigens recognized by CD4+ T cells, an approach was developed that allows the screening of an invariant chain-complementary DNA fusion library in a genetically engineered cell line expressing the essential components of the major histocompatibility complex (MHC) class II processing and presentation pathway. This led to the identification of a mutated form of human CDC27, which gave rise to an HLA- DR4-restricted melanoma antigen. A mutated form of triosephosphate isomerase, isolated by a biochemical method, was also identified as an HLA-DR1- restricted antigen. Thus, this approach may be generally applicable to the identification of antigens recognized by CD4+ T cells, which could aid the development of strategies for the treatment of patients with cancer, autoimmune diseases, or infectious diseases.

Original languageEnglish (US)
Pages (from-to)1351-1354
Number of pages4
JournalScience
Volume284
Issue number5418
DOIs
StatePublished - May 21 1999
Externally publishedYes

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Histocompatibility Antigens Class II
Neoplasm Antigens
Major Histocompatibility Complex
Organism Cloning
HLA-DR1 Antigen
Triose-Phosphate Isomerase
HLA-DR4 Antigen
Melanoma-Specific Antigens
T-Lymphocytes
CD4 Antigens
Gene Library
Genes
Autoimmune Diseases
Communicable Diseases
Complementary DNA
Cell Line
Neoplasms
Therapeutics
invariant chain

ASJC Scopus subject areas

  • General

Cite this

Cloning genes encoding MHC class II-restricted antigens : Mutated CDC27 as a tumor antigen. / Wang, Rong Fu; Wang, Xiang; Atwood, Alicia C.; Topalian, Suzanne; Rosenberg, Steven A.

In: Science, Vol. 284, No. 5418, 21.05.1999, p. 1351-1354.

Research output: Contribution to journalArticle

Wang, Rong Fu ; Wang, Xiang ; Atwood, Alicia C. ; Topalian, Suzanne ; Rosenberg, Steven A. / Cloning genes encoding MHC class II-restricted antigens : Mutated CDC27 as a tumor antigen. In: Science. 1999 ; Vol. 284, No. 5418. pp. 1351-1354.
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