Cloning genes encoding MHC class II-restricted antigens: Mutated CDC27 as a tumor antigen

Rong Fu Wang, Xiang Wang, Alicia C. Atwood, Suzanne L. Topalian, Steven A. Rosenberg

Research output: Contribution to journalArticle

Abstract

In an effort to identify tumor-specific antigens recognized by CD4+ T cells, an approach was developed that allows the screening of an invariant chain-complementary DNA fusion library in a genetically engineered cell line expressing the essential components of the major histocompatibility complex (MHC) class II processing and presentation pathway. This led to the identification of a mutated form of human CDC27, which gave rise to an HLA- DR4-restricted melanoma antigen. A mutated form of triosephosphate isomerase, isolated by a biochemical method, was also identified as an HLA-DR1- restricted antigen. Thus, this approach may be generally applicable to the identification of antigens recognized by CD4+ T cells, which could aid the development of strategies for the treatment of patients with cancer, autoimmune diseases, or infectious diseases.

Original languageEnglish (US)
Pages (from-to)1351-1354
Number of pages4
JournalScience
Volume284
Issue number5418
DOIs
StatePublished - May 21 1999

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