Cloning, expression and subcellular localization of HN1 and HN1L genes, as well as characterization of their orthologs, defining an evolutionarily conserved gene family

Guangjin Zhou; Jian Wang; Yue Zhang; Chaomin Zhong; Jun Ni; Li Wang; Jinhu Guo; Kexiong Zhang; Long Yu; Shouyuan Zhao

doi:10.1016/j.gene.2004.02.025

Cloning, expression and subcellular localization of HN1 and HN1L genes, as well as characterization of their orthologs, defining an evolutionarily conserved gene family

Guangjin Zhou, Jian Wang, Yue Zhang, Chaomin Zhong, Jun Ni, Li Wang, Jinhu Guo, Kexiong Zhang, Long Yu, Shouyuan Zhao

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

The present work reported the cloning and characterization of two novel human genes - HN1 (hematopoietic- and neurologic-expressed sequence 1) and HN1L (HN1-like gene) which are proposed to be involved in embryo development. HN1 is mapped on chromosome 17q25.2, with two transcripts (1.0 and 1.6 kb in length, respectively) due to alternative splicing. HN1 is expressed abundantly in testis and skeletal muscle among 16 human tissues, and it is localized in the nucleus indicated by GFP fusion expression. Western blot confirmed that HN1 encodes a 16.5-kDa protein. HN1L is on chromosome 16p13.3, with three splicing in the length of 2.0, 4.0 and 4.2 kb, respectively. HN1L is expressed in a variety of tissues such as liver, kidney, prostate, testis and uterus at varying levels. HN1L gene encodes a 20-kDa protein, which is localized in both the nucleus and cytoplasm. Fourteen of HN1 and sixteen of HN1L homologous genes in different species were determined and analyzed by BLAST searches. Silicon analyses of the 14 orthologous proteins of HN1 and 16 orthologous proteins of HN1L revealed that they share great conservation in vertebrate. Additionally, we identified nine pseudogenes of HN1 (six) and HN1L (three) in the genomes of the human, mouse and rat. Based on sequence alignments and phylogenetic analysis, all these homologous genes and pseudogenes were defined as a HN1 gene family.

Original language	English (US)
Pages (from-to)	115-123
Number of pages	9
Journal	Gene
Volume	331
Issue number	1-2
DOIs	https://doi.org/10.1016/j.gene.2004.02.025
State	Published - Apr 28 2004
Externally published	Yes

Keywords

4,6-diamidino-2-phenylindole
Amino acids
BLAST
BLAST-like Alignment Tool
BLAT
Base pair(s)
Basic Local Alignment Search Tool
Complementary DNA
DAPI
EST
Gene expression
Gene family
HN1
HN1L
Pseudogene
Subcellular localization
Western blot
aa
bp
cDNA

ASJC Scopus subject areas

Genetics

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10.1016/j.gene.2004.02.025

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@article{37ea93747d9b4b1abff1c4549cbd3a96,

title = "Cloning, expression and subcellular localization of HN1 and HN1L genes, as well as characterization of their orthologs, defining an evolutionarily conserved gene family",

abstract = "The present work reported the cloning and characterization of two novel human genes - HN1 (hematopoietic- and neurologic-expressed sequence 1) and HN1L (HN1-like gene) which are proposed to be involved in embryo development. HN1 is mapped on chromosome 17q25.2, with two transcripts (1.0 and 1.6 kb in length, respectively) due to alternative splicing. HN1 is expressed abundantly in testis and skeletal muscle among 16 human tissues, and it is localized in the nucleus indicated by GFP fusion expression. Western blot confirmed that HN1 encodes a 16.5-kDa protein. HN1L is on chromosome 16p13.3, with three splicing in the length of 2.0, 4.0 and 4.2 kb, respectively. HN1L is expressed in a variety of tissues such as liver, kidney, prostate, testis and uterus at varying levels. HN1L gene encodes a 20-kDa protein, which is localized in both the nucleus and cytoplasm. Fourteen of HN1 and sixteen of HN1L homologous genes in different species were determined and analyzed by BLAST searches. Silicon analyses of the 14 orthologous proteins of HN1 and 16 orthologous proteins of HN1L revealed that they share great conservation in vertebrate. Additionally, we identified nine pseudogenes of HN1 (six) and HN1L (three) in the genomes of the human, mouse and rat. Based on sequence alignments and phylogenetic analysis, all these homologous genes and pseudogenes were defined as a HN1 gene family.",

keywords = "4,6-diamidino-2-phenylindole, Amino acids, BLAST, BLAST-like Alignment Tool, BLAT, Base pair(s), Basic Local Alignment Search Tool, Complementary DNA, DAPI, EST, Gene expression, Gene family, HN1, HN1L, Pseudogene, Subcellular localization, Western blot, aa, bp, cDNA",

author = "Guangjin Zhou and Jian Wang and Yue Zhang and Chaomin Zhong and Jun Ni and Li Wang and Jinhu Guo and Kexiong Zhang and Long Yu and Shouyuan Zhao",

note = "Funding Information: We thank Prof. Yang Zhong for his help in drawing phylogenetic tree, and Prof. Chaoqun Wu and Prof. Edoardo Boncinelli for their critical reading of this manuscript. We also thank Dr. Z.L. Zhang, L. Dias and N. Gaugler for revision of the English. This work was supported by the National 973 Program (G1998051006) of China, 863 projects (2001AA221081) of China and the National Natural Science foundation of China (30024001, 30000095).",

year = "2004",

month = apr,

day = "28",

doi = "10.1016/j.gene.2004.02.025",

language = "English (US)",

volume = "331",

pages = "115--123",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier",

number = "1-2",

}

TY - JOUR

T1 - Cloning, expression and subcellular localization of HN1 and HN1L genes, as well as characterization of their orthologs, defining an evolutionarily conserved gene family

AU - Zhou, Guangjin

AU - Wang, Jian

AU - Zhang, Yue

AU - Zhong, Chaomin

AU - Ni, Jun

AU - Wang, Li

AU - Guo, Jinhu

AU - Zhang, Kexiong

AU - Yu, Long

AU - Zhao, Shouyuan

N1 - Funding Information: We thank Prof. Yang Zhong for his help in drawing phylogenetic tree, and Prof. Chaoqun Wu and Prof. Edoardo Boncinelli for their critical reading of this manuscript. We also thank Dr. Z.L. Zhang, L. Dias and N. Gaugler for revision of the English. This work was supported by the National 973 Program (G1998051006) of China, 863 projects (2001AA221081) of China and the National Natural Science foundation of China (30024001, 30000095).

PY - 2004/4/28

Y1 - 2004/4/28

N2 - The present work reported the cloning and characterization of two novel human genes - HN1 (hematopoietic- and neurologic-expressed sequence 1) and HN1L (HN1-like gene) which are proposed to be involved in embryo development. HN1 is mapped on chromosome 17q25.2, with two transcripts (1.0 and 1.6 kb in length, respectively) due to alternative splicing. HN1 is expressed abundantly in testis and skeletal muscle among 16 human tissues, and it is localized in the nucleus indicated by GFP fusion expression. Western blot confirmed that HN1 encodes a 16.5-kDa protein. HN1L is on chromosome 16p13.3, with three splicing in the length of 2.0, 4.0 and 4.2 kb, respectively. HN1L is expressed in a variety of tissues such as liver, kidney, prostate, testis and uterus at varying levels. HN1L gene encodes a 20-kDa protein, which is localized in both the nucleus and cytoplasm. Fourteen of HN1 and sixteen of HN1L homologous genes in different species were determined and analyzed by BLAST searches. Silicon analyses of the 14 orthologous proteins of HN1 and 16 orthologous proteins of HN1L revealed that they share great conservation in vertebrate. Additionally, we identified nine pseudogenes of HN1 (six) and HN1L (three) in the genomes of the human, mouse and rat. Based on sequence alignments and phylogenetic analysis, all these homologous genes and pseudogenes were defined as a HN1 gene family.

AB - The present work reported the cloning and characterization of two novel human genes - HN1 (hematopoietic- and neurologic-expressed sequence 1) and HN1L (HN1-like gene) which are proposed to be involved in embryo development. HN1 is mapped on chromosome 17q25.2, with two transcripts (1.0 and 1.6 kb in length, respectively) due to alternative splicing. HN1 is expressed abundantly in testis and skeletal muscle among 16 human tissues, and it is localized in the nucleus indicated by GFP fusion expression. Western blot confirmed that HN1 encodes a 16.5-kDa protein. HN1L is on chromosome 16p13.3, with three splicing in the length of 2.0, 4.0 and 4.2 kb, respectively. HN1L is expressed in a variety of tissues such as liver, kidney, prostate, testis and uterus at varying levels. HN1L gene encodes a 20-kDa protein, which is localized in both the nucleus and cytoplasm. Fourteen of HN1 and sixteen of HN1L homologous genes in different species were determined and analyzed by BLAST searches. Silicon analyses of the 14 orthologous proteins of HN1 and 16 orthologous proteins of HN1L revealed that they share great conservation in vertebrate. Additionally, we identified nine pseudogenes of HN1 (six) and HN1L (three) in the genomes of the human, mouse and rat. Based on sequence alignments and phylogenetic analysis, all these homologous genes and pseudogenes were defined as a HN1 gene family.

KW - 4,6-diamidino-2-phenylindole

KW - Amino acids

KW - BLAST

KW - BLAST-like Alignment Tool

KW - BLAT

KW - Base pair(s)

KW - Basic Local Alignment Search Tool

KW - Complementary DNA

KW - DAPI

KW - EST

KW - Gene expression

KW - Gene family

KW - HN1

KW - HN1L

KW - Pseudogene

KW - Subcellular localization

KW - Western blot

KW - aa

KW - bp

KW - cDNA

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U2 - 10.1016/j.gene.2004.02.025

DO - 10.1016/j.gene.2004.02.025

M3 - Article

C2 - 15094197

AN - SCOPUS:16544394767

SN - 0378-1119

VL - 331

SP - 115

EP - 123

JO - Gene

JF - Gene

IS - 1-2

ER -

Cloning, expression and subcellular localization of HN1 and HN1L genes, as well as characterization of their orthologs, defining an evolutionarily conserved gene family

Abstract

Keywords

ASJC Scopus subject areas

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