Cloning and sequencing the HinfI restriction and modification genes

Srinivasan Chandrasegaran, Keith D. Lunnen, Hamilton O. Smith, Geoffrey G. Wilson

Research output: Contribution to journalArticlepeer-review

Abstract

The HinfI restriction and modification genes were cloned on a 3.9-kb PstI fragment inserted into the PstI site of plasmid pBR322. Both genes are confined to an internal 2.3-kb BclI-AvaI subfragment. This subfragment was sequenced. Two large open reading frames (ORF's) are present. ORF1 codes for the methylase [predicted 359 amino acids (aa)] and ORF2 codes for the endonuclease (predicted 262 or 272 aa).

Original languageEnglish (US)
Pages (from-to)387-392
Number of pages6
JournalGene
Volume70
Issue number2
DOIs
StatePublished - Oct 30 1988

Keywords

  • Escherichia coli
  • Haemophilus influenzae
  • methylase
  • plasmids pBR322 and pUC19
  • recombinant DNA
  • restriction endonuclease

ASJC Scopus subject areas

  • Genetics

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