Cloning and molecular characterization of two mosquito iron regulatory proteins

D. Zhang, George Dimopoulos, A. Wolf, B. Miñana, F. C. Kafatos, J. J. Winzerling

Research output: Contribution to journalArticle

Abstract

Iron regulatory proteins (IRPs) control the synthesis of various proteins at the translational level by binding to iron responsive elements (IREs) in the mRNAs. Iron, infection, and stress can alter IRP/IRE binding activity. Insect messenger RNAs for ferritin and succinate dehydrogenase subunit b have IREs that are active translational control sites. We have cloned and sequenced cDNAs encoding proteins from the IRP1 family for the mosquitoes, Aedes aegypti and Anopheles gambiae. Both deduced amino acid sequences show substantial similarity to human IRP1 and Drosophila IRP1A and IRP1B, and all of the residues thought to be involved in aconitase activity and iron-sulfur cluster formation are conserved. Recombinant A. aegypti IRP1 binds to transcripts of the IREs of mosquito or human ferritin subunit mRNAs. No significant change in A. gambiae IRP1 messenger RNA could be detected during the various developmental stages of the life cycle, following iron loading by blood feeding, or after bacterial or parasitic infections. These data suggest that there is no change in gene transcription. Furthermore, bacterial challenge of A. gambiae cells did not change IRP1 protein levels. In contrast, IRP1 binding activity for the IRE was elevated following immune induction. These data show that changes in IRP1/IRE binding activity occur as part of the insect immune response.

Original languageEnglish (US)
Pages (from-to)579-589
Number of pages11
JournalInsect Biochemistry and Molecular Biology
Volume32
Issue number5
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

Iron-Regulatory Proteins
Cloning
regulatory proteins
Molecular Cloning
Culicidae
molecular cloning
Iron
iron
Anopheles gambiae
Iron Regulatory Protein 1
Messenger RNA
Ferritins
ferritin
Insects
messenger RNA
Aedes aegypti
succinate dehydrogenase (quinone)
Aconitate Hydratase
Parasitic Diseases
Succinate Dehydrogenase

Keywords

  • Infection
  • Iron
  • Iron regulatory protein
  • Mosquitoes

ASJC Scopus subject areas

  • Insect Science
  • Biochemistry

Cite this

Cloning and molecular characterization of two mosquito iron regulatory proteins. / Zhang, D.; Dimopoulos, George; Wolf, A.; Miñana, B.; Kafatos, F. C.; Winzerling, J. J.

In: Insect Biochemistry and Molecular Biology, Vol. 32, No. 5, 2002, p. 579-589.

Research output: Contribution to journalArticle

Zhang, D. ; Dimopoulos, George ; Wolf, A. ; Miñana, B. ; Kafatos, F. C. ; Winzerling, J. J. / Cloning and molecular characterization of two mosquito iron regulatory proteins. In: Insect Biochemistry and Molecular Biology. 2002 ; Vol. 32, No. 5. pp. 579-589.
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