Cloning and Expression of Goldfish Opsin Sequences

Randy L. Johnson, Kathryn B. Grant, Todd C. Zankel, Marcus F. Boehm, Koji Nakanishi, Shannath L Merbs, Jeremy Nathans, Jeremy Nathans

Research output: Contribution to journalArticle

Abstract

Five opsin cDNA clones were isolated from a goldfish retina cDNA library and sequenced. On the basis of homology to previously characterized visual pigments, one clone was identified as goldfish rod opsin and a second as a goldfish red cone opsin. Two rhodopsin-like clones were found to be similar to the chicken green opsin, a pigment which shares properties with both rod and cone pigments. A fifth clone was equally homologous to human blue cone opsin and human rod opsin. In order to characterize the spectral properties of the encoded pigments, the five clones were expressed in tissue culture cells and the apoproteins reconstituted with 11 -cis-retinal. The wavelength of maximal absorption for goldfish rhodopsin is 492 nm and for the fifth pigment, identified as the goldfish blue pigment, 441 nm. Pigments encoded by the two rhodopsin-like clones absorb at 505 and 511 nm and are likely to correspond to the goldfish green pigment previously characterized by microspectrophotometry. The putative red cone opsin cDNA may encode a pigment that is a polymorphic variant of goldfish red since it absorbs maximally at 525 nm.

Original languageEnglish (US)
Pages (from-to)208-214
Number of pages7
JournalBiochemistry
Volume32
Issue number1
DOIs
StatePublished - Jan 1 1993

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Opsins
Goldfish
Cloning
Pigments
Organism Cloning
Cone Opsins
Clone Cells
Rhodopsin
Rod Opsins
Complementary DNA
Microspectrophotometry
Retinaldehyde
Vertebrate Photoreceptor Cells
Apoproteins
Retinal Pigments
Tissue culture
Gene Library
Retina
Chickens
Cell Culture Techniques

ASJC Scopus subject areas

  • Biochemistry

Cite this

Johnson, R. L., Grant, K. B., Zankel, T. C., Boehm, M. F., Nakanishi, K., Merbs, S. L., ... Nathans, J. (1993). Cloning and Expression of Goldfish Opsin Sequences. Biochemistry, 32(1), 208-214. https://doi.org/10.1021/bi00052a027

Cloning and Expression of Goldfish Opsin Sequences. / Johnson, Randy L.; Grant, Kathryn B.; Zankel, Todd C.; Boehm, Marcus F.; Nakanishi, Koji; Merbs, Shannath L; Nathans, Jeremy; Nathans, Jeremy.

In: Biochemistry, Vol. 32, No. 1, 01.01.1993, p. 208-214.

Research output: Contribution to journalArticle

Johnson, RL, Grant, KB, Zankel, TC, Boehm, MF, Nakanishi, K, Merbs, SL, Nathans, J & Nathans, J 1993, 'Cloning and Expression of Goldfish Opsin Sequences', Biochemistry, vol. 32, no. 1, pp. 208-214. https://doi.org/10.1021/bi00052a027
Johnson RL, Grant KB, Zankel TC, Boehm MF, Nakanishi K, Merbs SL et al. Cloning and Expression of Goldfish Opsin Sequences. Biochemistry. 1993 Jan 1;32(1):208-214. https://doi.org/10.1021/bi00052a027
Johnson, Randy L. ; Grant, Kathryn B. ; Zankel, Todd C. ; Boehm, Marcus F. ; Nakanishi, Koji ; Merbs, Shannath L ; Nathans, Jeremy ; Nathans, Jeremy. / Cloning and Expression of Goldfish Opsin Sequences. In: Biochemistry. 1993 ; Vol. 32, No. 1. pp. 208-214.
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