Cloning and expression of a cocaine-sensitive dopamine transporter complementary DNA

Shoichi Shimada; Shigeo Kitayama; Chien Liang Lin; Amrat Patel; Elizabeth Nanthakumar; Paul Gregor; Michael Kuhar; George Uhl

Cloning and expression of a cocaine-sensitive dopamine transporter complementary DNA

Shoichi Shimada, Shigeo Kitayama, Chien Liang Lin, Amrat Patel, Elizabeth Nanthakumar, Paul Gregor, Michael Kuhar, George Uhl

School of Medicine

Research output: Contribution to journal › Article › peer-review

489 Scopus citations

Abstract

A rat dopamine (DA) transporter complementary DNA has been isolated with combined complementary DNA homology and expression approaches. The DA transporter is a 619-amino acid protein with 12 hydrophobic putative membrane-spanning domains and homology to the norepinephrine and γ-aminobutyric acid transporters. The expressed complementary DNA confers transport of [³H]DA in Xenopus oocytes and in COS cells. Binding of the cocaine analog [³H]CFT {[³H]2β-carbomethoxy-3β-(4-fluorophenyl)tropane} to transfected COS cell membranes yields a pharmacological profile similar to that in striatal membranes.

Original language	English (US)
Pages (from-to)	576-578
Number of pages	3
Journal	Science
Volume	254
Issue number	5031
State	Published - Oct 25 1991

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title = "Cloning and expression of a cocaine-sensitive dopamine transporter complementary DNA",

abstract = "A rat dopamine (DA) transporter complementary DNA has been isolated with combined complementary DNA homology and expression approaches. The DA transporter is a 619-amino acid protein with 12 hydrophobic putative membrane-spanning domains and homology to the norepinephrine and γ-aminobutyric acid transporters. The expressed complementary DNA confers transport of [3H]DA in Xenopus oocytes and in COS cells. Binding of the cocaine analog [3H]CFT {[3H]2β-carbomethoxy-3β-(4-fluorophenyl)tropane} to transfected COS cell membranes yields a pharmacological profile similar to that in striatal membranes.",

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T1 - Cloning and expression of a cocaine-sensitive dopamine transporter complementary DNA

AU - Shimada, Shoichi

AU - Kitayama, Shigeo

AU - Lin, Chien Liang

AU - Patel, Amrat

AU - Nanthakumar, Elizabeth

AU - Gregor, Paul

AU - Kuhar, Michael

AU - Uhl, George

PY - 1991/10/25

Y1 - 1991/10/25

N2 - A rat dopamine (DA) transporter complementary DNA has been isolated with combined complementary DNA homology and expression approaches. The DA transporter is a 619-amino acid protein with 12 hydrophobic putative membrane-spanning domains and homology to the norepinephrine and γ-aminobutyric acid transporters. The expressed complementary DNA confers transport of [3H]DA in Xenopus oocytes and in COS cells. Binding of the cocaine analog [3H]CFT {[3H]2β-carbomethoxy-3β-(4-fluorophenyl)tropane} to transfected COS cell membranes yields a pharmacological profile similar to that in striatal membranes.

AB - A rat dopamine (DA) transporter complementary DNA has been isolated with combined complementary DNA homology and expression approaches. The DA transporter is a 619-amino acid protein with 12 hydrophobic putative membrane-spanning domains and homology to the norepinephrine and γ-aminobutyric acid transporters. The expressed complementary DNA confers transport of [3H]DA in Xenopus oocytes and in COS cells. Binding of the cocaine analog [3H]CFT {[3H]2β-carbomethoxy-3β-(4-fluorophenyl)tropane} to transfected COS cell membranes yields a pharmacological profile similar to that in striatal membranes.

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JF - Science

IS - 5031

ER -

Cloning and expression of a cocaine-sensitive dopamine transporter complementary DNA

Abstract

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