Cloning and expression of a cocaine-sensitive dopamine transporter complementary DNA

Shoichi Shimada, Shigeo Kitayama, Chien Liang Lin, Amrat Patel, Elizabeth Nanthakumar, Paul Gregor, Michael Kuhar, George Uhl

Research output: Contribution to journalArticlepeer-review

Abstract

A rat dopamine (DA) transporter complementary DNA has been isolated with combined complementary DNA homology and expression approaches. The DA transporter is a 619-amino acid protein with 12 hydrophobic putative membrane-spanning domains and homology to the norepinephrine and γ-aminobutyric acid transporters. The expressed complementary DNA confers transport of [3H]DA in Xenopus oocytes and in COS cells. Binding of the cocaine analog [3H]CFT {[3H]2β-carbomethoxy-3β-(4-fluorophenyl)tropane} to transfected COS cell membranes yields a pharmacological profile similar to that in striatal membranes.

Original languageEnglish (US)
Pages (from-to)576-578
Number of pages3
JournalScience
Volume254
Issue number5031
StatePublished - Oct 25 1991

ASJC Scopus subject areas

  • General

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