Cloning and characterization of the human urea transporter UT-A1 and mapping of the human Slc14a2 gene

Serena M. Bagnasco, Tao Peng, Michael G. Janech, Alexander Karakashian, Jeff M. Sands

Research output: Contribution to journalArticle

Abstract

We have isolated and characterized the human homolog of the rat largest urea transporter of the UT-A family (hUT-A1). The 4.2-kb hUT-A1 cDNA encodes a 920-amino acid peptide, which is 89% identical to the rat UT-A1 protein. By Northern hybridization, hUT-A1 expression is detected in the human inner medulla as a ∼4.4-kb mRNA transcript. By Western analysis, hUT-A1 is identified as a ∼100-kDa protein in the human inner medulla. By immunohistochemistry, hUT-A1 expression is localized to the inner medullary collecting duct (IMCD). When transfected into HEK-293 cells hUT-A1 cDNA is translated into a ∼98-kDa protein. Expression of hUT-A1 in Xenopus oocytes results in phloretin-inhibitable uptake of 14C-urea, which shows only modest stimulation by cAMP, suggesting that in the human IMCD vasopressin may have a limited role in the short-term regulation of hUT-A1-mediated urea transport. We determined the organization of the human Slc14a2 gene and identified 20 exons distributed over ∼67.5 kb on chromosome 18, from which hUT-A1 and the other human urea transporter, hUT-A2, are transcribed.

Original languageEnglish (US)
Pages (from-to)F400-F406
JournalAmerican Journal of Physiology - Renal Physiology
Volume281
Issue number3 50-3
DOIs
StatePublished - 2001

Keywords

  • Kidney
  • Urea transport
  • Urine transporter
  • Vasopressin

ASJC Scopus subject areas

  • Physiology
  • Urology

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