Cloning and characterization of human polyamine-modulated factor-1, a transcriptional cofactor that regulates the transcription of the spermidine/spermine N1-acetyltransferase gene

Yanlin Wang, Wendy Devereux, Tracy Murray Stewart, Robert A Casero

Research output: Contribution to journalArticle

Abstract

The increased transcription and ultimate superinduction of the spermidine/spermine N1-acetyltransferase (SSAT) gene has been associated with the antineoplastic activity of several new antitumor polyamine analogues. In sensitive tumor cell types, the transcriptional induction appears to be regulated by the constitutive association of the transcription factor Nrf-2 with the recently discovered polyamine-responsive element. Using the yeast two-hybrid system, a new transcriptional cofactor, polyamine- modulated factor-1 (PMF-1), has been identified as a partner protein of Nrf-2 that, in combination with Nrf-2, regulates the polyamine analogue-induced transcription of SSAT. The human PMF-1 gene, located on chromosome 1 near the 1q12/1q21 border, yields an mRNA transcript of ~1.2 kilobases that codes for a 165-amino acid protein with a predicted molecular mass of ~20 kDa. The PMF-1 mRNA appears to increase in response to analogue exposure only in analogue-responsive cells. In addition to the transcriptional regulation of SSAT, PMF-1 or similar factors should be considered in the regulation of other polyamine-dependent genes.

Original languageEnglish (US)
Pages (from-to)22095-22101
Number of pages7
JournalJournal of Biological Chemistry
Volume274
Issue number31
DOIs
StatePublished - Jul 30 1999

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Acetyltransferases
Spermidine
Spermine
Cloning
Polyamines
Transcription
Organism Cloning
Genes
Messenger RNA
Two-Hybrid System Techniques
diamine N-acetyltransferase
Chromosomes, Human, Pair 1
Molecular mass
Chromosomes
Hybrid systems
Antineoplastic Agents
Yeast
Tumors
Proteins
Transcription Factors

ASJC Scopus subject areas

  • Biochemistry

Cite this

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title = "Cloning and characterization of human polyamine-modulated factor-1, a transcriptional cofactor that regulates the transcription of the spermidine/spermine N1-acetyltransferase gene",
abstract = "The increased transcription and ultimate superinduction of the spermidine/spermine N1-acetyltransferase (SSAT) gene has been associated with the antineoplastic activity of several new antitumor polyamine analogues. In sensitive tumor cell types, the transcriptional induction appears to be regulated by the constitutive association of the transcription factor Nrf-2 with the recently discovered polyamine-responsive element. Using the yeast two-hybrid system, a new transcriptional cofactor, polyamine- modulated factor-1 (PMF-1), has been identified as a partner protein of Nrf-2 that, in combination with Nrf-2, regulates the polyamine analogue-induced transcription of SSAT. The human PMF-1 gene, located on chromosome 1 near the 1q12/1q21 border, yields an mRNA transcript of ~1.2 kilobases that codes for a 165-amino acid protein with a predicted molecular mass of ~20 kDa. The PMF-1 mRNA appears to increase in response to analogue exposure only in analogue-responsive cells. In addition to the transcriptional regulation of SSAT, PMF-1 or similar factors should be considered in the regulation of other polyamine-dependent genes.",
author = "Yanlin Wang and Wendy Devereux and {Murray Stewart}, Tracy and Casero, {Robert A}",
year = "1999",
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T1 - Cloning and characterization of human polyamine-modulated factor-1, a transcriptional cofactor that regulates the transcription of the spermidine/spermine N1-acetyltransferase gene

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AU - Devereux, Wendy

AU - Murray Stewart, Tracy

AU - Casero, Robert A

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N2 - The increased transcription and ultimate superinduction of the spermidine/spermine N1-acetyltransferase (SSAT) gene has been associated with the antineoplastic activity of several new antitumor polyamine analogues. In sensitive tumor cell types, the transcriptional induction appears to be regulated by the constitutive association of the transcription factor Nrf-2 with the recently discovered polyamine-responsive element. Using the yeast two-hybrid system, a new transcriptional cofactor, polyamine- modulated factor-1 (PMF-1), has been identified as a partner protein of Nrf-2 that, in combination with Nrf-2, regulates the polyamine analogue-induced transcription of SSAT. The human PMF-1 gene, located on chromosome 1 near the 1q12/1q21 border, yields an mRNA transcript of ~1.2 kilobases that codes for a 165-amino acid protein with a predicted molecular mass of ~20 kDa. The PMF-1 mRNA appears to increase in response to analogue exposure only in analogue-responsive cells. In addition to the transcriptional regulation of SSAT, PMF-1 or similar factors should be considered in the regulation of other polyamine-dependent genes.

AB - The increased transcription and ultimate superinduction of the spermidine/spermine N1-acetyltransferase (SSAT) gene has been associated with the antineoplastic activity of several new antitumor polyamine analogues. In sensitive tumor cell types, the transcriptional induction appears to be regulated by the constitutive association of the transcription factor Nrf-2 with the recently discovered polyamine-responsive element. Using the yeast two-hybrid system, a new transcriptional cofactor, polyamine- modulated factor-1 (PMF-1), has been identified as a partner protein of Nrf-2 that, in combination with Nrf-2, regulates the polyamine analogue-induced transcription of SSAT. The human PMF-1 gene, located on chromosome 1 near the 1q12/1q21 border, yields an mRNA transcript of ~1.2 kilobases that codes for a 165-amino acid protein with a predicted molecular mass of ~20 kDa. The PMF-1 mRNA appears to increase in response to analogue exposure only in analogue-responsive cells. In addition to the transcriptional regulation of SSAT, PMF-1 or similar factors should be considered in the regulation of other polyamine-dependent genes.

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