Cloning and characterization of dnaE, encoding the catalytic subunit of replicative DNA polymerase III, from Vibrio cholerae strain C6706

Augusto A. Franco, Peir En Yeh, Judith A. Johnson, Eileen M. Barry, Humberto Guerra, Russell Maurer, J. Glenn Morris

    Research output: Contribution to journalArticlepeer-review

    Abstract

    We report that Vibrio cholerae (Vc) contains a gene homologous to Escherichia coli dnaE, the structural gene for the a (catalytic) subunit of replicative DNA polymerase III (PolIII). Despite 24% amino acid (aa) differences in the encoded proteins, the Vc gene strongly complements an E. coli dnaE temperature sensitive (ts) mutant, indicating that all functional features essential for replication are conserved.

    Original languageEnglish (US)
    Pages (from-to)281-283
    Number of pages3
    JournalGene
    Volume175
    Issue number1-2
    DOIs
    StatePublished - Oct 10 1996

    Keywords

    • Cholera
    • Escherichia coli
    • Recombinant DNA
    • Salmonella typhimurium

    ASJC Scopus subject areas

    • Genetics

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