Cloning and characterization of cDNAs coding for heavy and light chains of a monoclonal antibody (MabB9) specific for human plasma apolipoprotein B-100

Ju Won Kwak, Byung Kwon Choi, Dong Ik Lee, Yong Koo Kang, Young Gyo Seo, Won Kyung Cho, Young Do Kim, Moon Hi Han

Research output: Contribution to journalArticlepeer-review

Abstract

Low-density lipoproteins (LDL) and LDL-cholesterol in human plasma are clinically evaluated as important markers, whose concentrations have positive correlations with the incidence of coronary artery diseases. Here, the heavy and light chain cDNAs for the Fab region of a murine monoclonal antibody MabB9 (γ2b, κ), which is specific for apolipoprotein B-100 of human plasma LDL, were cloned and their nucleotide sequences were characterized. The sequence analyses revealed that the variable region of the heavy chain is a member of mouse heavy chain subgroup II(B) and that of the light chain is a member of κ subgroup V. Complementarity-determining regions (CDRs) 1, 2, and 3 in the variable regions of both chains were identified. Some unusual framework residues in the heavy chain were also identified. The sequence analyses suggest that the cDNAs are functional.

Original languageEnglish (US)
Pages (from-to)499-503
Number of pages5
JournalMolecules and cells
Volume6
Issue number4
StatePublished - Aug 31 1996

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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