Low-density lipoproteins (LDL) and LDL-cholesterol in human plasma are clinically evaluated as important markers, whose concentrations have positive correlations with the incidence of coronary artery diseases. Here, the heavy and light chain cDNAs for the Fab region of a murine monoclonal antibody MabB9 (γ2b, κ), which is specific for apolipoprotein B-100 of human plasma LDL, were cloned and their nucleotide sequences were characterized. The sequence analyses revealed that the variable region of the heavy chain is a member of mouse heavy chain subgroup II(B) and that of the light chain is a member of κ subgroup V. Complementarity-determining regions (CDRs) 1, 2, and 3 in the variable regions of both chains were identified. Some unusual framework residues in the heavy chain were also identified. The sequence analyses suggest that the cDNAs are functional.
|Original language||English (US)|
|Number of pages||5|
|Journal||Molecules and cells|
|State||Published - Aug 31 1996|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology