Cloning and characterization of allograft inflammatory factor-1: A novel macrophage factor identified in rat cardiac allografts with chronic rejection

U. Utans, R. J. Arceci, Y. Yamashita, M. E. Russell

Research output: Contribution to journalArticle

Abstract

The development of arteriosclerotic lesions in the Lewis to F344 rat model of chronic cardiac rejection is characterized by macrophage adhesion to the vessel lumen and macrophage infiltration in the neointima prior to smooth muscle cell accumulation. We report the cloning and characterization of allograft inflammatory factor-1 (AIF-1), a novel cDNA that is expressed early and persistently in chronically rejecting cardiac allografts but is absent in cardiac syngrafts and host hearts. The full-length cDNA codes for a hydrophilic polypeptide of 17 kD that contains a 12-amino acid region similar to an EF-hand (calcium-binding) domain. In cardiac allografts AIF-1 transcripts and protein localized to infiltrating mononuclear cells. Analysis of isolated cell populations confirmed that AIF-1 was selectively expressed in macrophages and neutrophils and demonstrated that AIF-1 transcripts could be upregulated by sixfold after stimulation with the T cell-derived cytokine IFN-γ. Treatment with a diet deficient in essential fatty acids (which attenuates arteriosclerosis) or CTLA-4 Ig (which blocks lymphocyte activation) significantly decreased AIF-1 transcript levels. Upregulation of AIF-1 in the setting of T cell activation suggests that it may play a role in macrophage activation and function.

Original languageEnglish (US)
Pages (from-to)2954-2962
Number of pages9
JournalJournal of Clinical Investigation
Volume95
Issue number6
StatePublished - 1995
Externally publishedYes

Fingerprint

Allografts
Organism Cloning
Macrophages
Complementary DNA
EF Hand Motifs
T-Lymphocytes
Neointima
Essential Fatty Acids
Macrophage Activation
Arteriosclerosis
Inbred F344 Rats
Lymphocyte Activation
Smooth Muscle Myocytes
Neutrophils
Up-Regulation
Cytokines
Diet
Calcium
Amino Acids
Peptides

Keywords

  • arteriosclerosis
  • cardiac transplantation
  • gene expression
  • macrophages
  • rejection

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Cloning and characterization of allograft inflammatory factor-1 : A novel macrophage factor identified in rat cardiac allografts with chronic rejection. / Utans, U.; Arceci, R. J.; Yamashita, Y.; Russell, M. E.

In: Journal of Clinical Investigation, Vol. 95, No. 6, 1995, p. 2954-2962.

Research output: Contribution to journalArticle

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