Cloning and characterization of a secreted frizzled-related protein that is expressed by the retinal pigment epithelium

Jinghua T. Chang, Noriko Esumi, Kathryn Moore, Yuanyuan Li, Suiyuan Zhang, Christina Chew, Barbara Goodman, Amir Rattner, Sally Moody, Gail Stetten, Peter A Campochiaro, Donald J Zack

Research output: Contribution to journalArticle

Abstract

The Wnt/frizzled cell signaling pathway has been implicated in the determination of polarity in a number of systems, including the Drosophila retina. The vertebrate retina develops from an undifferentiated neuroepithelium into an organized and laminated structure that demonstrates a high degree of polarity at both the tissue and cellular levels. In the process of searching for molecules that are preferentially expressed by the vertebrate retinal pigment epithelium (RPE), we identified secreted frizzled-related protein 5 (SFRP5), a member of the SFRP family that appears to act by modulating Wnt signal transduction. SFRP5 is highly expressed by RPE cells, and is also expressed in the pancreas. Within the retina, the related molecule SFRP2 is expressed specifically by cells of the inner nuclear layer. Thus, photoreceptors are likely to be bathed by two opposing gradients of SFRP molecules. Consistent with SFRP5's postulated role in modulating Wnt signaling in the retina, it inhibits the ability of Xwnt-8 mRNA to induce axis duplication in Xenopus embryos. The human SFRP5 gene consists of three coding exons and it maps to chromosome 10q24.1; human SFRP2 maps to 4q31.3. Based on the biology and complementary expression patterns of SFRP2 and SFRP5, we suggest that they may be involved in determining the polarity of photoreceptor, and perhaps other, cells in the retina.

Original languageEnglish (US)
Pages (from-to)575-583
Number of pages9
JournalHuman Molecular Genetics
Volume8
Issue number4
StatePublished - 1999

Fingerprint

Retinal Pigment Epithelium
Retina
Organism Cloning
Vertebrates
Aptitude
Human Chromosomes
Xenopus
Drosophila
Pancreas
Exons
Signal Transduction
Embryonic Structures
FRZB protein
Messenger RNA
Genes

ASJC Scopus subject areas

  • Genetics

Cite this

Cloning and characterization of a secreted frizzled-related protein that is expressed by the retinal pigment epithelium. / Chang, Jinghua T.; Esumi, Noriko; Moore, Kathryn; Li, Yuanyuan; Zhang, Suiyuan; Chew, Christina; Goodman, Barbara; Rattner, Amir; Moody, Sally; Stetten, Gail; Campochiaro, Peter A; Zack, Donald J.

In: Human Molecular Genetics, Vol. 8, No. 4, 1999, p. 575-583.

Research output: Contribution to journalArticle

Chang, Jinghua T. ; Esumi, Noriko ; Moore, Kathryn ; Li, Yuanyuan ; Zhang, Suiyuan ; Chew, Christina ; Goodman, Barbara ; Rattner, Amir ; Moody, Sally ; Stetten, Gail ; Campochiaro, Peter A ; Zack, Donald J. / Cloning and characterization of a secreted frizzled-related protein that is expressed by the retinal pigment epithelium. In: Human Molecular Genetics. 1999 ; Vol. 8, No. 4. pp. 575-583.
@article{4de62cef6cac4801a9fcf1dcb63bdd43,
title = "Cloning and characterization of a secreted frizzled-related protein that is expressed by the retinal pigment epithelium",
abstract = "The Wnt/frizzled cell signaling pathway has been implicated in the determination of polarity in a number of systems, including the Drosophila retina. The vertebrate retina develops from an undifferentiated neuroepithelium into an organized and laminated structure that demonstrates a high degree of polarity at both the tissue and cellular levels. In the process of searching for molecules that are preferentially expressed by the vertebrate retinal pigment epithelium (RPE), we identified secreted frizzled-related protein 5 (SFRP5), a member of the SFRP family that appears to act by modulating Wnt signal transduction. SFRP5 is highly expressed by RPE cells, and is also expressed in the pancreas. Within the retina, the related molecule SFRP2 is expressed specifically by cells of the inner nuclear layer. Thus, photoreceptors are likely to be bathed by two opposing gradients of SFRP molecules. Consistent with SFRP5's postulated role in modulating Wnt signaling in the retina, it inhibits the ability of Xwnt-8 mRNA to induce axis duplication in Xenopus embryos. The human SFRP5 gene consists of three coding exons and it maps to chromosome 10q24.1; human SFRP2 maps to 4q31.3. Based on the biology and complementary expression patterns of SFRP2 and SFRP5, we suggest that they may be involved in determining the polarity of photoreceptor, and perhaps other, cells in the retina.",
author = "Chang, {Jinghua T.} and Noriko Esumi and Kathryn Moore and Yuanyuan Li and Suiyuan Zhang and Christina Chew and Barbara Goodman and Amir Rattner and Sally Moody and Gail Stetten and Campochiaro, {Peter A} and Zack, {Donald J}",
year = "1999",
language = "English (US)",
volume = "8",
pages = "575--583",
journal = "Human Molecular Genetics",
issn = "0964-6906",
publisher = "Oxford University Press",
number = "4",

}

TY - JOUR

T1 - Cloning and characterization of a secreted frizzled-related protein that is expressed by the retinal pigment epithelium

AU - Chang, Jinghua T.

AU - Esumi, Noriko

AU - Moore, Kathryn

AU - Li, Yuanyuan

AU - Zhang, Suiyuan

AU - Chew, Christina

AU - Goodman, Barbara

AU - Rattner, Amir

AU - Moody, Sally

AU - Stetten, Gail

AU - Campochiaro, Peter A

AU - Zack, Donald J

PY - 1999

Y1 - 1999

N2 - The Wnt/frizzled cell signaling pathway has been implicated in the determination of polarity in a number of systems, including the Drosophila retina. The vertebrate retina develops from an undifferentiated neuroepithelium into an organized and laminated structure that demonstrates a high degree of polarity at both the tissue and cellular levels. In the process of searching for molecules that are preferentially expressed by the vertebrate retinal pigment epithelium (RPE), we identified secreted frizzled-related protein 5 (SFRP5), a member of the SFRP family that appears to act by modulating Wnt signal transduction. SFRP5 is highly expressed by RPE cells, and is also expressed in the pancreas. Within the retina, the related molecule SFRP2 is expressed specifically by cells of the inner nuclear layer. Thus, photoreceptors are likely to be bathed by two opposing gradients of SFRP molecules. Consistent with SFRP5's postulated role in modulating Wnt signaling in the retina, it inhibits the ability of Xwnt-8 mRNA to induce axis duplication in Xenopus embryos. The human SFRP5 gene consists of three coding exons and it maps to chromosome 10q24.1; human SFRP2 maps to 4q31.3. Based on the biology and complementary expression patterns of SFRP2 and SFRP5, we suggest that they may be involved in determining the polarity of photoreceptor, and perhaps other, cells in the retina.

AB - The Wnt/frizzled cell signaling pathway has been implicated in the determination of polarity in a number of systems, including the Drosophila retina. The vertebrate retina develops from an undifferentiated neuroepithelium into an organized and laminated structure that demonstrates a high degree of polarity at both the tissue and cellular levels. In the process of searching for molecules that are preferentially expressed by the vertebrate retinal pigment epithelium (RPE), we identified secreted frizzled-related protein 5 (SFRP5), a member of the SFRP family that appears to act by modulating Wnt signal transduction. SFRP5 is highly expressed by RPE cells, and is also expressed in the pancreas. Within the retina, the related molecule SFRP2 is expressed specifically by cells of the inner nuclear layer. Thus, photoreceptors are likely to be bathed by two opposing gradients of SFRP molecules. Consistent with SFRP5's postulated role in modulating Wnt signaling in the retina, it inhibits the ability of Xwnt-8 mRNA to induce axis duplication in Xenopus embryos. The human SFRP5 gene consists of three coding exons and it maps to chromosome 10q24.1; human SFRP2 maps to 4q31.3. Based on the biology and complementary expression patterns of SFRP2 and SFRP5, we suggest that they may be involved in determining the polarity of photoreceptor, and perhaps other, cells in the retina.

UR - http://www.scopus.com/inward/record.url?scp=0032998688&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032998688&partnerID=8YFLogxK

M3 - Article

C2 - 10072424

AN - SCOPUS:0032998688

VL - 8

SP - 575

EP - 583

JO - Human Molecular Genetics

JF - Human Molecular Genetics

SN - 0964-6906

IS - 4

ER -