TY - JOUR
T1 - Cloning and characterization of a secreted frizzled-related protein that is expressed by the retinal pigment epithelium
AU - Chang, Jinghua T.
AU - Esumi, Noriko
AU - Moore, Kathryn
AU - Li, Yuanyuan
AU - Zhang, Suiyuan
AU - Chew, Christina
AU - Goodman, Barbara
AU - Rattner, Amir
AU - Moody, Sally
AU - Stetten, Gail
AU - Campochiaro, Peter A.
AU - Zack, Donald J.
PY - 1999
Y1 - 1999
N2 - The Wnt/frizzled cell signaling pathway has been implicated in the determination of polarity in a number of systems, including the Drosophila retina. The vertebrate retina develops from an undifferentiated neuroepithelium into an organized and laminated structure that demonstrates a high degree of polarity at both the tissue and cellular levels. In the process of searching for molecules that are preferentially expressed by the vertebrate retinal pigment epithelium (RPE), we identified secreted frizzled-related protein 5 (SFRP5), a member of the SFRP family that appears to act by modulating Wnt signal transduction. SFRP5 is highly expressed by RPE cells, and is also expressed in the pancreas. Within the retina, the related molecule SFRP2 is expressed specifically by cells of the inner nuclear layer. Thus, photoreceptors are likely to be bathed by two opposing gradients of SFRP molecules. Consistent with SFRP5's postulated role in modulating Wnt signaling in the retina, it inhibits the ability of Xwnt-8 mRNA to induce axis duplication in Xenopus embryos. The human SFRP5 gene consists of three coding exons and it maps to chromosome 10q24.1; human SFRP2 maps to 4q31.3. Based on the biology and complementary expression patterns of SFRP2 and SFRP5, we suggest that they may be involved in determining the polarity of photoreceptor, and perhaps other, cells in the retina.
AB - The Wnt/frizzled cell signaling pathway has been implicated in the determination of polarity in a number of systems, including the Drosophila retina. The vertebrate retina develops from an undifferentiated neuroepithelium into an organized and laminated structure that demonstrates a high degree of polarity at both the tissue and cellular levels. In the process of searching for molecules that are preferentially expressed by the vertebrate retinal pigment epithelium (RPE), we identified secreted frizzled-related protein 5 (SFRP5), a member of the SFRP family that appears to act by modulating Wnt signal transduction. SFRP5 is highly expressed by RPE cells, and is also expressed in the pancreas. Within the retina, the related molecule SFRP2 is expressed specifically by cells of the inner nuclear layer. Thus, photoreceptors are likely to be bathed by two opposing gradients of SFRP molecules. Consistent with SFRP5's postulated role in modulating Wnt signaling in the retina, it inhibits the ability of Xwnt-8 mRNA to induce axis duplication in Xenopus embryos. The human SFRP5 gene consists of three coding exons and it maps to chromosome 10q24.1; human SFRP2 maps to 4q31.3. Based on the biology and complementary expression patterns of SFRP2 and SFRP5, we suggest that they may be involved in determining the polarity of photoreceptor, and perhaps other, cells in the retina.
UR - http://www.scopus.com/inward/record.url?scp=0032998688&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032998688&partnerID=8YFLogxK
U2 - 10.1093/hmg/8.4.575
DO - 10.1093/hmg/8.4.575
M3 - Article
C2 - 10072424
AN - SCOPUS:0032998688
SN - 0964-6906
VL - 8
SP - 575
EP - 583
JO - Human molecular genetics
JF - Human molecular genetics
IS - 4
ER -