Cloning and characterization of a novel gene, SHPRH, encoding a conserved putative protein with SNF2/helicase and PHD-finger domains from the 6q24 region

Raman Sood, Izabela Makalowska, Michal Galdzicki, Ping Hu, Erica Eddings, Christiane M. Robbins, Tracy Moses, Jin Namkoong, Suzie Chen, Jeffrey M. Trent

Research output: Contribution to journalArticlepeer-review

Abstract

Here we report the identification of a novel transcript containing SNF2, PHD-finger, RING-finger, helicase, and linker histone domains mapping to the q24 band region of human chromosome 6. These domains are characteristic of several DNA repair proteins, transcription factors, and helicases. We have cloned both human and mouse homologs of this novel gene using interexon PCR and RACE technologies. The human cDNA, termed SHPRH, is 6018 bp and codes for a putative protein of 1683 amino acids. The mouse cDNA, termed Shprh, is 7225 bp and codes for a putative protein of 1616 amino acids. The deduced amino acid sequences of the two proteins share 86% identity. Both genes are expressed ubiquitously, with a transcript size of ∼7.5 kb. Mapping of this gene to 6q24, a region reported to contain a tumor suppressor locus, prompted us to evaluate SHPRH by mutation analysis in tumor cell lines. We have identified one truncating and three missense mutations, thus suggesting SHPRH as a possible candidate for the tumor suppressor gene.

Original languageEnglish (US)
Pages (from-to)153-161
Number of pages9
JournalGenomics
Volume82
Issue number2
DOIs
StatePublished - Aug 1 2003
Externally publishedYes

Keywords

  • 6q24
  • DNA/RNA helicases
  • Linker histone domain
  • PHD-finger domain
  • RING-finger domain
  • SHPRH
  • SNF2 domain
  • Tumor suppressor

ASJC Scopus subject areas

  • Genetics

Fingerprint

Dive into the research topics of 'Cloning and characterization of a novel gene, SHPRH, encoding a conserved putative protein with SNF2/helicase and PHD-finger domains from the 6q24 region'. Together they form a unique fingerprint.

Cite this