Cloning and characterization of a human polyamine oxidase that is inducible by polyamine analogue exposure

Y. Wang, W. Devereux, P. M. Woster, Tracy Murray Stewart, A. Hacker, Robert A Casero

Research output: Contribution to journalArticle

Abstract

Mammalian polyamine catabolism is under the control of two enzymes, spermidine/spermine N1-acetyltransferase and the flavin adenine dinucleotide-dependent polyamine oxidase (PAO). In this study, the cloning and initial characterization of human PAO is reported. A 1894-bp cDNA with an open reading frame of 1668-bp codes for a protein of 555 amino acids. In vitro transcription/translation of this cDNA clone produces the expected Mr 61,900 protein with PAO activity. The PAO activity of this clone is inhibited by MDL 72,527, a specific inhibitor of mammalian PAO. However, neither pargyline, a specific monoamine oxidase inhibitor, nor semicarbazide, a specific diamine oxidase inhibitor, inhibits the PAO activity of this clone. PAO has been referred to as being constitutively expressed. However, 24-h exposure of a non-small cell lung carcinoma cell line, NCI H157, to 10 μM of N1,N″-bis(ethyl)norspermine results in ∼5-fold induction of PAO mRNA and a > 3-fold induction of PAO activity. These results demonstrate that in at least one cell type, PAO is up-regulated in response to polyamine analogue exposure. The PAO clone described here should provide a useful tool, which will facilitate the dissection of the role of polyamine catabolism in normal growth and in response to the antitumor polyamine analogues.

Original languageEnglish (US)
Pages (from-to)5370-5373
Number of pages4
JournalCancer Research
Volume61
Issue number14
StatePublished - Jul 15 2001

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Polyamines
Organism Cloning
Clone Cells
polyamine oxidase
Complementary DNA
Pargyline
Amine Oxidase (Copper-Containing)
Flavin-Adenine Dinucleotide
Monoamine Oxidase Inhibitors
Spermine
Non-Small Cell Lung Carcinoma
Open Reading Frames
Dissection
Proteins
Amino Acids
Cell Line
Messenger RNA

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Cloning and characterization of a human polyamine oxidase that is inducible by polyamine analogue exposure. / Wang, Y.; Devereux, W.; Woster, P. M.; Murray Stewart, Tracy; Hacker, A.; Casero, Robert A.

In: Cancer Research, Vol. 61, No. 14, 15.07.2001, p. 5370-5373.

Research output: Contribution to journalArticle

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AU - Devereux, W.

AU - Woster, P. M.

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AU - Hacker, A.

AU - Casero, Robert A

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