Cloning and analysis of a cecropin gene from the malaria vector mosquito, Anopheles gambiae

J. Vizioli, P. Bulet, M. Charlet, C. Lowenberger, C. Blass, H. M. Müller, George Dimopoulos, J. Hoffmann, F. C. Kafatos, Adam Richman

Research output: Contribution to journalArticle

Abstract

Parasites of the genus Plasmodium are transmitted to mammalian hosts by anopheline mosquitoes. Within the insect vector, parasite growth and development are potentially limited by antimicrobial defence molecules. Here, we describe the isolation of cDNA and genomic clones encoding a cecropin antibacterial peptide from the malaria vector mosquito Anopheles gambiae. The locus was mapped to polytene division 1C of the X chromosome. Cecropin RNA was induced by infection with bacteria and Plasmodium. RNA levels varied in different body parts of the adult mosquito. During development, cecropin expression was limited to the early pupal stage. The peptide was purified from both adult mosquitoes and cell culture supernatants. Anopheles gambiae synthetic cecropins displayed activity against Gram-positive bacteria, filamentous fungi and yeasts.

Original languageEnglish (US)
Pages (from-to)75-84
Number of pages10
JournalInsect Molecular Biology
Volume9
Issue number1
DOIs
StatePublished - Feb 2000
Externally publishedYes

Fingerprint

Cecropins
Anopheles gambiae
Cloning
malaria
Malaria
Organism Cloning
molecular cloning
Culicidae
Genes
Plasmodium
Bacteria
Parasites
genes
RNA
Insect Vectors
parasites
Peptides
insect vectors
antimicrobial peptides
X Chromosome

Keywords

  • Anopheles gambiae
  • Antimicrobial peptide
  • Cecropin
  • Innate immunity
  • Malaria

ASJC Scopus subject areas

  • Insect Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Vizioli, J., Bulet, P., Charlet, M., Lowenberger, C., Blass, C., Müller, H. M., ... Richman, A. (2000). Cloning and analysis of a cecropin gene from the malaria vector mosquito, Anopheles gambiae. Insect Molecular Biology, 9(1), 75-84. https://doi.org/10.1046/j.1365-2583.2000.00164.x

Cloning and analysis of a cecropin gene from the malaria vector mosquito, Anopheles gambiae. / Vizioli, J.; Bulet, P.; Charlet, M.; Lowenberger, C.; Blass, C.; Müller, H. M.; Dimopoulos, George; Hoffmann, J.; Kafatos, F. C.; Richman, Adam.

In: Insect Molecular Biology, Vol. 9, No. 1, 02.2000, p. 75-84.

Research output: Contribution to journalArticle

Vizioli, J, Bulet, P, Charlet, M, Lowenberger, C, Blass, C, Müller, HM, Dimopoulos, G, Hoffmann, J, Kafatos, FC & Richman, A 2000, 'Cloning and analysis of a cecropin gene from the malaria vector mosquito, Anopheles gambiae', Insect Molecular Biology, vol. 9, no. 1, pp. 75-84. https://doi.org/10.1046/j.1365-2583.2000.00164.x
Vizioli, J. ; Bulet, P. ; Charlet, M. ; Lowenberger, C. ; Blass, C. ; Müller, H. M. ; Dimopoulos, George ; Hoffmann, J. ; Kafatos, F. C. ; Richman, Adam. / Cloning and analysis of a cecropin gene from the malaria vector mosquito, Anopheles gambiae. In: Insect Molecular Biology. 2000 ; Vol. 9, No. 1. pp. 75-84.
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