Abstract
Parasites of the genus Plasmodium are transmitted to mammalian hosts by anopheline mosquitoes. Within the insect vector, parasite growth and development are potentially limited by antimicrobial defence molecules. Here, we describe the isolation of cDNA and genomic clones encoding a cecropin antibacterial peptide from the malaria vector mosquito Anopheles gambiae. The locus was mapped to polytene division 1C of the X chromosome. Cecropin RNA was induced by infection with bacteria and Plasmodium. RNA levels varied in different body parts of the adult mosquito. During development, cecropin expression was limited to the early pupal stage. The peptide was purified from both adult mosquitoes and cell culture supernatants. Anopheles gambiae synthetic cecropins displayed activity against Gram-positive bacteria, filamentous fungi and yeasts.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 75-84 |
| Number of pages | 10 |
| Journal | Insect Molecular Biology |
| Volume | 9 |
| Issue number | 1 |
| DOIs | |
| State | Published - Feb 2000 |
| Externally published | Yes |
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Keywords
- Anopheles gambiae
- Antimicrobial peptide
- Cecropin
- Innate immunity
- Malaria
ASJC Scopus subject areas
- Insect Science
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
Cite this
Cloning and analysis of a cecropin gene from the malaria vector mosquito, Anopheles gambiae. / Vizioli, J.; Bulet, P.; Charlet, M.; Lowenberger, C.; Blass, C.; Müller, H. M.; Dimopoulos, George; Hoffmann, J.; Kafatos, F. C.; Richman, Adam.
In: Insect Molecular Biology, Vol. 9, No. 1, 02.2000, p. 75-84.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cloning and analysis of a cecropin gene from the malaria vector mosquito, Anopheles gambiae
AU - Vizioli, J.
AU - Bulet, P.
AU - Charlet, M.
AU - Lowenberger, C.
AU - Blass, C.
AU - Müller, H. M.
AU - Dimopoulos, George
AU - Hoffmann, J.
AU - Kafatos, F. C.
AU - Richman, Adam
PY - 2000/2
Y1 - 2000/2
N2 - Parasites of the genus Plasmodium are transmitted to mammalian hosts by anopheline mosquitoes. Within the insect vector, parasite growth and development are potentially limited by antimicrobial defence molecules. Here, we describe the isolation of cDNA and genomic clones encoding a cecropin antibacterial peptide from the malaria vector mosquito Anopheles gambiae. The locus was mapped to polytene division 1C of the X chromosome. Cecropin RNA was induced by infection with bacteria and Plasmodium. RNA levels varied in different body parts of the adult mosquito. During development, cecropin expression was limited to the early pupal stage. The peptide was purified from both adult mosquitoes and cell culture supernatants. Anopheles gambiae synthetic cecropins displayed activity against Gram-positive bacteria, filamentous fungi and yeasts.
AB - Parasites of the genus Plasmodium are transmitted to mammalian hosts by anopheline mosquitoes. Within the insect vector, parasite growth and development are potentially limited by antimicrobial defence molecules. Here, we describe the isolation of cDNA and genomic clones encoding a cecropin antibacterial peptide from the malaria vector mosquito Anopheles gambiae. The locus was mapped to polytene division 1C of the X chromosome. Cecropin RNA was induced by infection with bacteria and Plasmodium. RNA levels varied in different body parts of the adult mosquito. During development, cecropin expression was limited to the early pupal stage. The peptide was purified from both adult mosquitoes and cell culture supernatants. Anopheles gambiae synthetic cecropins displayed activity against Gram-positive bacteria, filamentous fungi and yeasts.
KW - Anopheles gambiae
KW - Antimicrobial peptide
KW - Cecropin
KW - Innate immunity
KW - Malaria
UR - http://www.scopus.com/inward/record.url?scp=0034144458&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034144458&partnerID=8YFLogxK
U2 - 10.1046/j.1365-2583.2000.00164.x
DO - 10.1046/j.1365-2583.2000.00164.x
M3 - Article
C2 - 10672074
AN - SCOPUS:0034144458
VL - 9
SP - 75
EP - 84
JO - Insect Molecular Biology
JF - Insect Molecular Biology
SN - 0962-1075
IS - 1
ER -