Cloning a novel member of the human interferon-inducible gene family associated with control of tumorigenicity in a model of human melanoma

Katherine L. DeYoung, Michael E. Ray, Yan A. Su, Sarah L. Anzick, Ricky W. Johnstone, Joseph A. Trapani, Paul S. Meltzer, Jeffrey M. Trent

Research output: Contribution to journalArticle

Abstract

Chromosome 6-mediated suppression of tumorigenicity in malignant melanoma cell lines provides a model system to identify genes associated with the reversion of the tumorigenic phenotype. Using subtractive cDNA selection, we recently identified a series of novel genes which are differentially expressed in association with chromosome 6-mediated suppression. We now report the molecular characterization of a novel gene termed AIM2 for (Absent In Melanoma), which represents a 1485 bp cDNA. An open reading frame of 1032 base pairs, corresponding to 344 amino acid residues, is predicted. The predicted protein shares a conserved sequence domain of approximately 200 amino acids with known interferon-inducible genes of both human and mouse. We demonstrate that the AIM2 gene encodes a transcript of approximately 2 kb which is expressed in spleen, small intestine, and peripheral blood leukocytes. In addition, we have localized AIM2 to the long arm of human chromosome 1 (band q22) in a highly conserved region which also contains the known interferon-inducible genes IFI16 and MNDA. We have also demonstrated that, like IFI16 and MNDA, AIM2 is induced in HL60 cells by interferon gamma. Our findings support the existence of a family of genes in this region similar to the well-characterized mouse Ifi200 gene family.

Original languageEnglish (US)
Pages (from-to)453-457
Number of pages5
JournalOncogene
Volume15
Issue number4
StatePublished - 1997
Externally publishedYes

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Keywords

  • Interferon-inducible
  • Melanoma
  • Tumorigenicity

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

DeYoung, K. L., Ray, M. E., Su, Y. A., Anzick, S. L., Johnstone, R. W., Trapani, J. A., Meltzer, P. S., & Trent, J. M. (1997). Cloning a novel member of the human interferon-inducible gene family associated with control of tumorigenicity in a model of human melanoma. Oncogene, 15(4), 453-457.