Cloned yeast and mammalian transcription factor TFIID gene products support basal but not activated metallothionein gene transcription

Ravi Kambadur, Valeria Culotta, Dean Hamer

Research output: Contribution to journalArticle

Abstract

Transcription factor IID (TFIID), the "TATA binding factor," is thought to play a key role in the regulation of eukaryotic transcriptional initiation. We have studied the role of TFIID in the transcription of the yeast metallothionein gene, which is regulated by the copper-dependent activator protein ACE1. Both basal and induced transcription of the metallothionein gene require TFIID and a functional TATA binding site. Crude human and mouse TFIID fractions, prepared from mammalian cells, respond to stimulation by ACE1. In contrast, human and yeast TFIID proteins expressed from the cloned genes do not respond to ACE1, except in the presence of wheat germ or yeast total cell extracts. These results indicate that the cloned TFIID gene products lack a component(s) or modification(s) that is required for regulated as compared to basal transcription.

Original languageEnglish (US)
Pages (from-to)9168-9172
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number23
DOIs
StatePublished - Jan 1 1990
Externally publishedYes

ASJC Scopus subject areas

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