Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase

David S. Bredt, Paul M. Hwang, Charles E. Glatt, Charles Lowenstein, Randall R Reed, Solomon H Snyder

Research output: Contribution to journalArticle

Abstract

Nitric oxide is a messenger molecule, mediating the effect of endothelium-derived relaxing factor in blood vessels and the cytotoxic actions of macrophages, and playing a part in neuronal communication in the brain. Cloning of a complementary DNA for brain nitric oxide synthase reveals recognition sites for NADPH, FAD, flavin mononucleotide and calmodulin as well as phosphorylation sites, indicating that the synthase is regulated by many different factors. The only known mammalian enzyme with close homology is cytochrome P-450 reductase.

Original languageEnglish (US)
Pages (from-to)714-718
Number of pages5
JournalNature
Volume351
Issue number6329
StatePublished - Jun 27 1991

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NADPH-Ferrihemoprotein Reductase
Nitric Oxide Synthase
Flavin Mononucleotide
Endothelium-Dependent Relaxing Factors
Flavin-Adenine Dinucleotide
Brain
Calmodulin
NADP
Blood Vessels
Organism Cloning
Nitric Oxide
Complementary DNA
Macrophages
Phosphorylation
Enzymes

ASJC Scopus subject areas

  • General

Cite this

Bredt, D. S., Hwang, P. M., Glatt, C. E., Lowenstein, C., Reed, R. R., & Snyder, S. H. (1991). Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase. Nature, 351(6329), 714-718.

Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase. / Bredt, David S.; Hwang, Paul M.; Glatt, Charles E.; Lowenstein, Charles; Reed, Randall R; Snyder, Solomon H.

In: Nature, Vol. 351, No. 6329, 27.06.1991, p. 714-718.

Research output: Contribution to journalArticle

Bredt, DS, Hwang, PM, Glatt, CE, Lowenstein, C, Reed, RR & Snyder, SH 1991, 'Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase', Nature, vol. 351, no. 6329, pp. 714-718.
Bredt, David S. ; Hwang, Paul M. ; Glatt, Charles E. ; Lowenstein, Charles ; Reed, Randall R ; Snyder, Solomon H. / Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase. In: Nature. 1991 ; Vol. 351, No. 6329. pp. 714-718.
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