Cloned and expressed macrophage nitric oxide synthase contrasts with the brain enzyme

Charles J. Lowenstein, Charles S. Glatt, David S. Bredt, Solomon H. Snyder

Research output: Contribution to journalArticlepeer-review

Abstract

Nitric oxide (NO) is a messenger molecule of macrophages, endothelial cells in blood vessels, and neurons. A neuronal form of NO synthase (NOS) has been previously cloned. We now report the molecular cloning of macrophage NOS. The macrophage enzyme displays 50% sequence identity to the neuronal enzyme. Like neuronal NOS, macrophage NOS has recognition sites for FAD, FMN, and NADPH and also has a consensus calmodulin binding site. Macrophage NOS mRNA is strikingly inducible; it is absent in quiescent macrophages or spleen but is prominent 2-6 hr after endotoxin treatment. (.

Original languageEnglish (US)
Pages (from-to)6711-6715
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number15
DOIs
StatePublished - 1992

Keywords

  • Endotoxin
  • Interferon
  • Kupffer cells
  • Long-term potentiation

ASJC Scopus subject areas

  • General

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