Cloned and expressed macrophage nitric oxide synthase contrasts with the brain enzyme

Charles J. Lowenstein; Charles S. Glatt; David S. Bredt; Solomon H. Snyder

doi:10.1073/pnas.89.15.6711

Cloned and expressed macrophage nitric oxide synthase contrasts with the brain enzyme

Charles J. Lowenstein, Charles S. Glatt, David S. Bredt, Solomon H. Snyder

School of Medicine

Research output: Contribution to journal › Article › peer-review

609 Scopus citations

Abstract

Nitric oxide (NO) is a messenger molecule of macrophages, endothelial cells in blood vessels, and neurons. A neuronal form of NO synthase (NOS) has been previously cloned. We now report the molecular cloning of macrophage NOS. The macrophage enzyme displays 50% sequence identity to the neuronal enzyme. Like neuronal NOS, macrophage NOS has recognition sites for FAD, FMN, and NADPH and also has a consensus calmodulin binding site. Macrophage NOS mRNA is strikingly inducible; it is absent in quiescent macrophages or spleen but is prominent 2-6 hr after endotoxin treatment. (.

Original language	English (US)
Pages (from-to)	6711-6715
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	89
Issue number	15
DOIs	https://doi.org/10.1073/pnas.89.15.6711
State	Published - 1992

Keywords

Endotoxin
Interferon
Kupffer cells
Long-term potentiation

ASJC Scopus subject areas

General

Access to Document

10.1073/pnas.89.15.6711

Cite this

@article{c535273eb6b84272b37dcd16d08d3e41,

title = "Cloned and expressed macrophage nitric oxide synthase contrasts with the brain enzyme",

abstract = "Nitric oxide (NO) is a messenger molecule of macrophages, endothelial cells in blood vessels, and neurons. A neuronal form of NO synthase (NOS) has been previously cloned. We now report the molecular cloning of macrophage NOS. The macrophage enzyme displays 50% sequence identity to the neuronal enzyme. Like neuronal NOS, macrophage NOS has recognition sites for FAD, FMN, and NADPH and also has a consensus calmodulin binding site. Macrophage NOS mRNA is strikingly inducible; it is absent in quiescent macrophages or spleen but is prominent 2-6 hr after endotoxin treatment. (.",

keywords = "Endotoxin, Interferon, Kupffer cells, Long-term potentiation",

author = "Lowenstein, {Charles J.} and Glatt, {Charles S.} and Bredt, {David S.} and Snyder, {Solomon H.}",

year = "1992",

doi = "10.1073/pnas.89.15.6711",

language = "English (US)",

volume = "89",

pages = "6711--6715",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

issn = "0027-8424",

publisher = "National Academy of Sciences",

number = "15",

}

TY - JOUR

T1 - Cloned and expressed macrophage nitric oxide synthase contrasts with the brain enzyme

AU - Lowenstein, Charles J.

AU - Glatt, Charles S.

AU - Bredt, David S.

AU - Snyder, Solomon H.

PY - 1992

Y1 - 1992

N2 - Nitric oxide (NO) is a messenger molecule of macrophages, endothelial cells in blood vessels, and neurons. A neuronal form of NO synthase (NOS) has been previously cloned. We now report the molecular cloning of macrophage NOS. The macrophage enzyme displays 50% sequence identity to the neuronal enzyme. Like neuronal NOS, macrophage NOS has recognition sites for FAD, FMN, and NADPH and also has a consensus calmodulin binding site. Macrophage NOS mRNA is strikingly inducible; it is absent in quiescent macrophages or spleen but is prominent 2-6 hr after endotoxin treatment. (.

AB - Nitric oxide (NO) is a messenger molecule of macrophages, endothelial cells in blood vessels, and neurons. A neuronal form of NO synthase (NOS) has been previously cloned. We now report the molecular cloning of macrophage NOS. The macrophage enzyme displays 50% sequence identity to the neuronal enzyme. Like neuronal NOS, macrophage NOS has recognition sites for FAD, FMN, and NADPH and also has a consensus calmodulin binding site. Macrophage NOS mRNA is strikingly inducible; it is absent in quiescent macrophages or spleen but is prominent 2-6 hr after endotoxin treatment. (.

KW - Endotoxin

KW - Interferon

KW - Kupffer cells

KW - Long-term potentiation

UR - http://www.scopus.com/inward/record.url?scp=0026729267&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026729267&partnerID=8YFLogxK

U2 - 10.1073/pnas.89.15.6711

DO - 10.1073/pnas.89.15.6711

M3 - Article

C2 - 1379716

AN - SCOPUS:0026729267

SN - 0027-8424

VL - 89

SP - 6711

EP - 6715

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 15

ER -

Cloned and expressed macrophage nitric oxide synthase contrasts with the brain enzyme

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this