Clonal precursor of bone, cartilage, and hematopoietic niche stromal cells

Charles K F Chan, Paul Lindau, Wen Jiang, James Y. Chen, Lillian F. Zhang, Ching Cheng Chen, Jun Seita, Debashis Sahoo, Jae Beom Kim, Andrew Lee, Sujin Park, Divya Nag, Yongquan Gong, Subhash Kulkarni, Cynthia A. Luppen, Alexander A. Theologis, Derrick C. Wan, Anthony DeBoer, Eun Young Seo, Justin D. Vincent-TompkinsKyle Loh, Graham G. Walmsley, Daniel L. Kraft, Joseph C. Wu, Michael T. Longaker, Irving L. Weissman

Research output: Contribution to journalArticle

Abstract

Organs are composites of tissue types with diverse developmental origins, and they rely on distinct stem and progenitor cells to meet physiological demands for cellular production and homeostasis. How diverse stem cell activity is coordinated within organs is not well understood. Here we describe a lineage-restricted, self-renewing common skeletal progenitor (bone, cartilage, stromal progenitor; BCSP) isolated from limb bones and bonemarrow tissue of fetal, neonatal, and adult mice. The BCSP clonally produces chondrocytes (cartilage-forming) and osteogenic (bone-forming) cells and at least three subsets of stromal cells that exhibit differential expression of cell surface markers, including CD105 (or endoglin), Thy1 [or CD90 (cluster of differentiation 90)], and 6C3 [ENPEP glutamyl aminopeptidase (aminopeptidase A)]. These three stromal subsets exhibit differential capacities to support hematopoietic (blood-forming) stem and progenitor cells. Although the 6C3-expressing subset demonstrates functional stem cell niche activity by maintaining primitive hematopoietic stem cell (HSC) renewal in vitro, the other stromal populations promote HSC differentiation to more committed lines of hematopoiesis, such as the B-cell lineage. Gene expression analysis and microscopic studies further reveal a microenvironment in which CD105-, Thy1-, and 6C3-expressing marrow stroma collaborate to provide cytokine signaling to HSCs and more committed hematopoietic progenitors. As a result, within the context of bone as a blood-forming organ, the BCSP plays a critical role in supporting hematopoiesis through its generation of diverse osteogenic and hematopoietic-promoting stroma, including HSC supportive 6C3(+) niche cells.

Original languageEnglish (US)
Pages (from-to)12643-12648
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume110
Issue number31
DOIs
StatePublished - Jul 30 2013
Externally publishedYes

Fingerprint

Stromal Cells
Cartilage
Stem Cells
Bone and Bones
Hematopoietic Stem Cells
Glutamyl Aminopeptidase
Hematopoiesis
Stem Cell Niche
Cell Lineage
Chondrocytes
Cell Differentiation
Homeostasis
B-Lymphocytes
Extremities
Bone Marrow
Cytokines
Gene Expression
Population

Keywords

  • Endochondral ossification
  • Lymphopoiesis

ASJC Scopus subject areas

  • General

Cite this

Chan, C. K. F., Lindau, P., Jiang, W., Chen, J. Y., Zhang, L. F., Chen, C. C., ... Weissman, I. L. (2013). Clonal precursor of bone, cartilage, and hematopoietic niche stromal cells. Proceedings of the National Academy of Sciences of the United States of America, 110(31), 12643-12648. https://doi.org/10.1073/pnas.1310212110

Clonal precursor of bone, cartilage, and hematopoietic niche stromal cells. / Chan, Charles K F; Lindau, Paul; Jiang, Wen; Chen, James Y.; Zhang, Lillian F.; Chen, Ching Cheng; Seita, Jun; Sahoo, Debashis; Kim, Jae Beom; Lee, Andrew; Park, Sujin; Nag, Divya; Gong, Yongquan; Kulkarni, Subhash; Luppen, Cynthia A.; Theologis, Alexander A.; Wan, Derrick C.; DeBoer, Anthony; Seo, Eun Young; Vincent-Tompkins, Justin D.; Loh, Kyle; Walmsley, Graham G.; Kraft, Daniel L.; Wu, Joseph C.; Longaker, Michael T.; Weissman, Irving L.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, No. 31, 30.07.2013, p. 12643-12648.

Research output: Contribution to journalArticle

Chan, CKF, Lindau, P, Jiang, W, Chen, JY, Zhang, LF, Chen, CC, Seita, J, Sahoo, D, Kim, JB, Lee, A, Park, S, Nag, D, Gong, Y, Kulkarni, S, Luppen, CA, Theologis, AA, Wan, DC, DeBoer, A, Seo, EY, Vincent-Tompkins, JD, Loh, K, Walmsley, GG, Kraft, DL, Wu, JC, Longaker, MT & Weissman, IL 2013, 'Clonal precursor of bone, cartilage, and hematopoietic niche stromal cells', Proceedings of the National Academy of Sciences of the United States of America, vol. 110, no. 31, pp. 12643-12648. https://doi.org/10.1073/pnas.1310212110
Chan, Charles K F ; Lindau, Paul ; Jiang, Wen ; Chen, James Y. ; Zhang, Lillian F. ; Chen, Ching Cheng ; Seita, Jun ; Sahoo, Debashis ; Kim, Jae Beom ; Lee, Andrew ; Park, Sujin ; Nag, Divya ; Gong, Yongquan ; Kulkarni, Subhash ; Luppen, Cynthia A. ; Theologis, Alexander A. ; Wan, Derrick C. ; DeBoer, Anthony ; Seo, Eun Young ; Vincent-Tompkins, Justin D. ; Loh, Kyle ; Walmsley, Graham G. ; Kraft, Daniel L. ; Wu, Joseph C. ; Longaker, Michael T. ; Weissman, Irving L. / Clonal precursor of bone, cartilage, and hematopoietic niche stromal cells. In: Proceedings of the National Academy of Sciences of the United States of America. 2013 ; Vol. 110, No. 31. pp. 12643-12648.
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AU - Seita, Jun

AU - Sahoo, Debashis

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AU - Lee, Andrew

AU - Park, Sujin

AU - Nag, Divya

AU - Gong, Yongquan

AU - Kulkarni, Subhash

AU - Luppen, Cynthia A.

AU - Theologis, Alexander A.

AU - Wan, Derrick C.

AU - DeBoer, Anthony

AU - Seo, Eun Young

AU - Vincent-Tompkins, Justin D.

AU - Loh, Kyle

AU - Walmsley, Graham G.

AU - Kraft, Daniel L.

AU - Wu, Joseph C.

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