TY - JOUR
T1 - Clonal immunoglobulin DNA in the plasma of patients with AIDS lymphoma
AU - Wagner-Johnston, Nina D.
AU - Gellert, Lan
AU - Gocke, Christopher D.
AU - Lemas, Victor M.
AU - Lee, Jeannette
AU - Martínez-Maza, Otoniel
AU - Ambinder, Richard F.
PY - 2011/5/5
Y1 - 2011/5/5
N2 - Immunoglobulin (Ig) gene rearrangements are used to define clonality of suspected B-lineage malignancy in tissue samples. To determine whether such rearrangements could be identified in plasma, we screened plasma from 14 consecutive patients with AIDS-related lymphoma with multiplex Ig primers. Clonally rearranged Ig DNA was detected in plasma from 7 of 14 patients. Patients in whom clonal Ig DNA remained detectable after combination chemotherapy died with lymphoma. Tumor was available from 1 patient, and the IgH amplification products from plasma and tumor were sequenced and confirmed to be identical. Ig DNA rearrangements in plasma may be useful as a lymphoma-specific tumor marker, and failure to clear clonal Ig DNA may identify patients at high risk for failure of standard therapy.
AB - Immunoglobulin (Ig) gene rearrangements are used to define clonality of suspected B-lineage malignancy in tissue samples. To determine whether such rearrangements could be identified in plasma, we screened plasma from 14 consecutive patients with AIDS-related lymphoma with multiplex Ig primers. Clonally rearranged Ig DNA was detected in plasma from 7 of 14 patients. Patients in whom clonal Ig DNA remained detectable after combination chemotherapy died with lymphoma. Tumor was available from 1 patient, and the IgH amplification products from plasma and tumor were sequenced and confirmed to be identical. Ig DNA rearrangements in plasma may be useful as a lymphoma-specific tumor marker, and failure to clear clonal Ig DNA may identify patients at high risk for failure of standard therapy.
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U2 - 10.1182/blood-2010-12-324657
DO - 10.1182/blood-2010-12-324657
M3 - Article
C2 - 21389324
AN - SCOPUS:79955945781
SN - 0006-4971
VL - 117
SP - 4860
EP - 4862
JO - Blood
JF - Blood
IS - 18
ER -