Clonal immunoglobulin DNA in the plasma of patients with AIDS lymphoma

Nina D. Wagner-Johnston, Lan Gellert, Christopher D. Gocke, Victor M. Lemas, Jeannette Lee, Otoniel Martínez-Maza, Richard F. Ambinder

Research output: Contribution to journalArticle

Abstract

Immunoglobulin (Ig) gene rearrangements are used to define clonality of suspected B-lineage malignancy in tissue samples. To determine whether such rearrangements could be identified in plasma, we screened plasma from 14 consecutive patients with AIDS-related lymphoma with multiplex Ig primers. Clonally rearranged Ig DNA was detected in plasma from 7 of 14 patients. Patients in whom clonal Ig DNA remained detectable after combination chemotherapy died with lymphoma. Tumor was available from 1 patient, and the IgH amplification products from plasma and tumor were sequenced and confirmed to be identical. Ig DNA rearrangements in plasma may be useful as a lymphoma-specific tumor marker, and failure to clear clonal Ig DNA may identify patients at high risk for failure of standard therapy.

Original languageEnglish (US)
Pages (from-to)4860-4862
Number of pages3
JournalBlood
Volume117
Issue number18
DOIs
StatePublished - May 5 2011

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Fingerprint Dive into the research topics of 'Clonal immunoglobulin DNA in the plasma of patients with AIDS lymphoma'. Together they form a unique fingerprint.

  • Cite this