Clonal immunoglobulin DNA in the plasma of patients with AIDS lymphoma

Nina Wagner-Johnston, Lan Gellert, Christopher Gocke, Mario Victor Lemas, Jeannette Lee, Otoniel Martínez-Maza, Richard F Ambinder

Research output: Contribution to journalArticle

Abstract

Immunoglobulin (Ig) gene rearrangements are used to define clonality of suspected B-lineage malignancy in tissue samples. To determine whether such rearrangements could be identified in plasma, we screened plasma from 14 consecutive patients with AIDS-related lymphoma with multiplex Ig primers. Clonally rearranged Ig DNA was detected in plasma from 7 of 14 patients. Patients in whom clonal Ig DNA remained detectable after combination chemotherapy died with lymphoma. Tumor was available from 1 patient, and the IgH amplification products from plasma and tumor were sequenced and confirmed to be identical. Ig DNA rearrangements in plasma may be useful as a lymphoma-specific tumor marker, and failure to clear clonal Ig DNA may identify patients at high risk for failure of standard therapy.

Original languageEnglish (US)
Pages (from-to)4860-4862
Number of pages3
JournalBlood
Volume117
Issue number18
DOIs
StatePublished - May 5 2011

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Immunoglobulins
Lymphoma
Acquired Immunodeficiency Syndrome
Plasmas
DNA
Gene Rearrangement
AIDS-Related Lymphoma
Tumors
Neoplasms
Immunoglobulin Genes
Tumor Biomarkers
Combination Drug Therapy
Chemotherapy
Amplification
Genes
Tissue
Therapeutics

ASJC Scopus subject areas

  • Hematology
  • Biochemistry
  • Cell Biology
  • Immunology

Cite this

Clonal immunoglobulin DNA in the plasma of patients with AIDS lymphoma. / Wagner-Johnston, Nina; Gellert, Lan; Gocke, Christopher; Lemas, Mario Victor; Lee, Jeannette; Martínez-Maza, Otoniel; Ambinder, Richard F.

In: Blood, Vol. 117, No. 18, 05.05.2011, p. 4860-4862.

Research output: Contribution to journalArticle

Wagner-Johnston, Nina ; Gellert, Lan ; Gocke, Christopher ; Lemas, Mario Victor ; Lee, Jeannette ; Martínez-Maza, Otoniel ; Ambinder, Richard F. / Clonal immunoglobulin DNA in the plasma of patients with AIDS lymphoma. In: Blood. 2011 ; Vol. 117, No. 18. pp. 4860-4862.
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