Clonal hematopoiesis in Philadelphia chromosome-negative bone marrow cells of chronic myeloid leukemia patients receiving dasatinib

Ronald L. Paquette; John Nicoll; Meenal Chalukya; Lucas Gondek; Monika Jasek; Charles L. Sawyers; Neil P. Shah; Jaroslaw Maciejewski

doi:10.1016/j.leukres.2009.08.032

Clonal hematopoiesis in Philadelphia chromosome-negative bone marrow cells of chronic myeloid leukemia patients receiving dasatinib

Ronald L. Paquette, John Nicoll, Meenal Chalukya, Lucas Gondek, Monika Jasek, Charles L. Sawyers, Neil P. Shah, Jaroslaw Maciejewski

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

A clonal cytogenetic abnormality was observed in Philadelphia chromosome-negative bone marrow cells of 6/27 chronic myeloid leukemia patients (+8 in 4, -7 in 1, and 20q- in 1) with dasatinib-induced remissions. The X-linked human androgen receptor gene assay demonstrated clonality in one additional patient. Single nucleotide polymorphism array analysis revealed somatic uniparental disomy involving chromosome 17(p12-pter) in another patient. The TP53 gene had a 5′ splice site deletion of exon 6 that caused alternative splicing, frame shifting and introduction of a premature stop codon. After three years, no patient developed myelodysplastic syndrome or acute myeloid leukemia.

Original language	English (US)
Pages (from-to)	708-713
Number of pages	6
Journal	Leukemia Research
Volume	34
Issue number	6
DOIs	https://doi.org/10.1016/j.leukres.2009.08.032
State	Published - Jun 2010
Externally published	Yes

Keywords

Chronic myeloid leukemia
Cytogenetics
Dasatinib
Myelodysplastic syndrome
Single nucleotide polymorphism array
TP53

ASJC Scopus subject areas

Hematology
Oncology
Cancer Research

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10.1016/j.leukres.2009.08.032

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title = "Clonal hematopoiesis in Philadelphia chromosome-negative bone marrow cells of chronic myeloid leukemia patients receiving dasatinib",

abstract = "A clonal cytogenetic abnormality was observed in Philadelphia chromosome-negative bone marrow cells of 6/27 chronic myeloid leukemia patients (+8 in 4, -7 in 1, and 20q- in 1) with dasatinib-induced remissions. The X-linked human androgen receptor gene assay demonstrated clonality in one additional patient. Single nucleotide polymorphism array analysis revealed somatic uniparental disomy involving chromosome 17(p12-pter) in another patient. The TP53 gene had a 5′ splice site deletion of exon 6 that caused alternative splicing, frame shifting and introduction of a premature stop codon. After three years, no patient developed myelodysplastic syndrome or acute myeloid leukemia.",

keywords = "Chronic myeloid leukemia, Cytogenetics, Dasatinib, Myelodysplastic syndrome, Single nucleotide polymorphism array, TP53",

author = "Paquette, {Ronald L.} and John Nicoll and Meenal Chalukya and Lucas Gondek and Monika Jasek and Sawyers, {Charles L.} and Shah, {Neil P.} and Jaroslaw Maciejewski",

note = "Funding Information: This research was supported by the NIH National Center for Research Resources. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the NIH. Additional support came from the Leukemia and Lymphoma Society, and the Greg Wojahn Memorial Fund.",

year = "2010",

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AU - Paquette, Ronald L.

AU - Nicoll, John

AU - Chalukya, Meenal

AU - Gondek, Lucas

AU - Jasek, Monika

AU - Sawyers, Charles L.

AU - Shah, Neil P.

AU - Maciejewski, Jaroslaw

N1 - Funding Information: This research was supported by the NIH National Center for Research Resources. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the NIH. Additional support came from the Leukemia and Lymphoma Society, and the Greg Wojahn Memorial Fund.

PY - 2010/6

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N2 - A clonal cytogenetic abnormality was observed in Philadelphia chromosome-negative bone marrow cells of 6/27 chronic myeloid leukemia patients (+8 in 4, -7 in 1, and 20q- in 1) with dasatinib-induced remissions. The X-linked human androgen receptor gene assay demonstrated clonality in one additional patient. Single nucleotide polymorphism array analysis revealed somatic uniparental disomy involving chromosome 17(p12-pter) in another patient. The TP53 gene had a 5′ splice site deletion of exon 6 that caused alternative splicing, frame shifting and introduction of a premature stop codon. After three years, no patient developed myelodysplastic syndrome or acute myeloid leukemia.

AB - A clonal cytogenetic abnormality was observed in Philadelphia chromosome-negative bone marrow cells of 6/27 chronic myeloid leukemia patients (+8 in 4, -7 in 1, and 20q- in 1) with dasatinib-induced remissions. The X-linked human androgen receptor gene assay demonstrated clonality in one additional patient. Single nucleotide polymorphism array analysis revealed somatic uniparental disomy involving chromosome 17(p12-pter) in another patient. The TP53 gene had a 5′ splice site deletion of exon 6 that caused alternative splicing, frame shifting and introduction of a premature stop codon. After three years, no patient developed myelodysplastic syndrome or acute myeloid leukemia.

KW - Chronic myeloid leukemia

KW - Cytogenetics

KW - Dasatinib

KW - Myelodysplastic syndrome

KW - Single nucleotide polymorphism array

KW - TP53

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Clonal hematopoiesis in Philadelphia chromosome-negative bone marrow cells of chronic myeloid leukemia patients receiving dasatinib

Abstract

Keywords

ASJC Scopus subject areas

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