Clonal analysis of myelodysplastic syndrome: Monosomy 7 is expressed in the myeloid lineage, but not in the lymphoid lineage as detected by fluorescent in situ hybridization

Winald R. Gerritsen, John Donohue, Jan Bauman, Suresh C. Jhanwar, Nancy A. Kernan, Hugo Castro-Malaspina, Richard J. O'Reilly, Jean Henri Bourhis

Research output: Contribution to journalArticle

Abstract

Conflicting results have been published on whether or not myelodysplastic syndromes (MDS) affect all cell lineages. Involvement of myeloid and erythroid cell lineages has been regularly observed, but it remains controversial whether the different lymphoid cell lineages are involved. In this study of eight patients with MDS associated with monosomy 7, fluorescent in situ hybridization (FISH) was used to enumerate the chromosomes 7 in interphase cells. With the probe D7Z1, the rate of false-positive detection of monosomy 7 was 3% ± 2% in normal cells. T- and B-cell lines were established from eight patients with MDS and monosomy 7. As determined by FISH in interphase cells, 1.9% (0% to 3%) of the cells in the B-cell lines showed one fluorescent spot and 1.1% (0% to 2.9%) of the cells in the T-cell lines. These values do not differ from normal values. However, the possibility that normal cells were selected when the T- and B-cell lines were established could not be excluded. Therefore, peripheral blood cells were obtained, separated according to surface markers specific for lymphoid and myeloid cell lineage with a cell sorter, and analyzed for the expression of monosomy 7 by FISH. Antibodies recognizing T cells (CD3), B cells (CD20), natural killer (NK) cells (CD57), monocytes and granulocytes (low and high expression of CD11b antigen), and myeloid progenitors (CD33) were used to separate cells. The expression of monosomy 7 in the T cells, NK cells, and B cells did not differ from control values. These results in the lymphoid subpopulations are in stark contrast with the observations in the myeloid populations; the percentage of cells with monosomy 7 ranged from 9% to 78% (controls: 6% ± 2%) in cells with low CD11b expression, 20% to 89% in cells with a high expression of the CD11b antigen (controls: 7% ± 3%), and 23% to 91% in the CD33 positive cells (controls: 5% ± 3%). The results of this study suggest that monosomy 7 does not usually affect lymphoid subpopulations but is restricted to committed progenitor cells with the capacity to differentiate into mature myeloid cells.

Original languageEnglish (US)
Pages (from-to)217-224
Number of pages8
JournalBlood
Volume80
Issue number1
StatePublished - Jul 1 1992
Externally publishedYes

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Myelodysplastic Syndromes
Fluorescence In Situ Hybridization
Cells
T-cells
CD11b Antigens
Cell Lineage
B-Lymphocytes
Myeloid Cells
Cell Line
Interphase
T-Lymphocytes
Natural Killer Cells
Chromosomes
Monosomy Chromosome 7
Lymphocytes
Blood
Erythroid Cells
Chromosomes, Human, Pair 7
Antibodies
Granulocytes

ASJC Scopus subject areas

  • Hematology

Cite this

Gerritsen, W. R., Donohue, J., Bauman, J., Jhanwar, S. C., Kernan, N. A., Castro-Malaspina, H., ... Bourhis, J. H. (1992). Clonal analysis of myelodysplastic syndrome: Monosomy 7 is expressed in the myeloid lineage, but not in the lymphoid lineage as detected by fluorescent in situ hybridization. Blood, 80(1), 217-224.

Clonal analysis of myelodysplastic syndrome : Monosomy 7 is expressed in the myeloid lineage, but not in the lymphoid lineage as detected by fluorescent in situ hybridization. / Gerritsen, Winald R.; Donohue, John; Bauman, Jan; Jhanwar, Suresh C.; Kernan, Nancy A.; Castro-Malaspina, Hugo; O'Reilly, Richard J.; Bourhis, Jean Henri.

In: Blood, Vol. 80, No. 1, 01.07.1992, p. 217-224.

Research output: Contribution to journalArticle

Gerritsen, WR, Donohue, J, Bauman, J, Jhanwar, SC, Kernan, NA, Castro-Malaspina, H, O'Reilly, RJ & Bourhis, JH 1992, 'Clonal analysis of myelodysplastic syndrome: Monosomy 7 is expressed in the myeloid lineage, but not in the lymphoid lineage as detected by fluorescent in situ hybridization', Blood, vol. 80, no. 1, pp. 217-224.
Gerritsen, Winald R. ; Donohue, John ; Bauman, Jan ; Jhanwar, Suresh C. ; Kernan, Nancy A. ; Castro-Malaspina, Hugo ; O'Reilly, Richard J. ; Bourhis, Jean Henri. / Clonal analysis of myelodysplastic syndrome : Monosomy 7 is expressed in the myeloid lineage, but not in the lymphoid lineage as detected by fluorescent in situ hybridization. In: Blood. 1992 ; Vol. 80, No. 1. pp. 217-224.
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abstract = "Conflicting results have been published on whether or not myelodysplastic syndromes (MDS) affect all cell lineages. Involvement of myeloid and erythroid cell lineages has been regularly observed, but it remains controversial whether the different lymphoid cell lineages are involved. In this study of eight patients with MDS associated with monosomy 7, fluorescent in situ hybridization (FISH) was used to enumerate the chromosomes 7 in interphase cells. With the probe D7Z1, the rate of false-positive detection of monosomy 7 was 3{\%} ± 2{\%} in normal cells. T- and B-cell lines were established from eight patients with MDS and monosomy 7. As determined by FISH in interphase cells, 1.9{\%} (0{\%} to 3{\%}) of the cells in the B-cell lines showed one fluorescent spot and 1.1{\%} (0{\%} to 2.9{\%}) of the cells in the T-cell lines. These values do not differ from normal values. However, the possibility that normal cells were selected when the T- and B-cell lines were established could not be excluded. Therefore, peripheral blood cells were obtained, separated according to surface markers specific for lymphoid and myeloid cell lineage with a cell sorter, and analyzed for the expression of monosomy 7 by FISH. Antibodies recognizing T cells (CD3), B cells (CD20), natural killer (NK) cells (CD57), monocytes and granulocytes (low and high expression of CD11b antigen), and myeloid progenitors (CD33) were used to separate cells. The expression of monosomy 7 in the T cells, NK cells, and B cells did not differ from control values. These results in the lymphoid subpopulations are in stark contrast with the observations in the myeloid populations; the percentage of cells with monosomy 7 ranged from 9{\%} to 78{\%} (controls: 6{\%} ± 2{\%}) in cells with low CD11b expression, 20{\%} to 89{\%} in cells with a high expression of the CD11b antigen (controls: 7{\%} ± 3{\%}), and 23{\%} to 91{\%} in the CD33 positive cells (controls: 5{\%} ± 3{\%}). The results of this study suggest that monosomy 7 does not usually affect lymphoid subpopulations but is restricted to committed progenitor cells with the capacity to differentiate into mature myeloid cells.",
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T2 - Monosomy 7 is expressed in the myeloid lineage, but not in the lymphoid lineage as detected by fluorescent in situ hybridization

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AU - Bauman, Jan

AU - Jhanwar, Suresh C.

AU - Kernan, Nancy A.

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AU - Bourhis, Jean Henri

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N2 - Conflicting results have been published on whether or not myelodysplastic syndromes (MDS) affect all cell lineages. Involvement of myeloid and erythroid cell lineages has been regularly observed, but it remains controversial whether the different lymphoid cell lineages are involved. In this study of eight patients with MDS associated with monosomy 7, fluorescent in situ hybridization (FISH) was used to enumerate the chromosomes 7 in interphase cells. With the probe D7Z1, the rate of false-positive detection of monosomy 7 was 3% ± 2% in normal cells. T- and B-cell lines were established from eight patients with MDS and monosomy 7. As determined by FISH in interphase cells, 1.9% (0% to 3%) of the cells in the B-cell lines showed one fluorescent spot and 1.1% (0% to 2.9%) of the cells in the T-cell lines. These values do not differ from normal values. However, the possibility that normal cells were selected when the T- and B-cell lines were established could not be excluded. Therefore, peripheral blood cells were obtained, separated according to surface markers specific for lymphoid and myeloid cell lineage with a cell sorter, and analyzed for the expression of monosomy 7 by FISH. Antibodies recognizing T cells (CD3), B cells (CD20), natural killer (NK) cells (CD57), monocytes and granulocytes (low and high expression of CD11b antigen), and myeloid progenitors (CD33) were used to separate cells. The expression of monosomy 7 in the T cells, NK cells, and B cells did not differ from control values. These results in the lymphoid subpopulations are in stark contrast with the observations in the myeloid populations; the percentage of cells with monosomy 7 ranged from 9% to 78% (controls: 6% ± 2%) in cells with low CD11b expression, 20% to 89% in cells with a high expression of the CD11b antigen (controls: 7% ± 3%), and 23% to 91% in the CD33 positive cells (controls: 5% ± 3%). The results of this study suggest that monosomy 7 does not usually affect lymphoid subpopulations but is restricted to committed progenitor cells with the capacity to differentiate into mature myeloid cells.

AB - Conflicting results have been published on whether or not myelodysplastic syndromes (MDS) affect all cell lineages. Involvement of myeloid and erythroid cell lineages has been regularly observed, but it remains controversial whether the different lymphoid cell lineages are involved. In this study of eight patients with MDS associated with monosomy 7, fluorescent in situ hybridization (FISH) was used to enumerate the chromosomes 7 in interphase cells. With the probe D7Z1, the rate of false-positive detection of monosomy 7 was 3% ± 2% in normal cells. T- and B-cell lines were established from eight patients with MDS and monosomy 7. As determined by FISH in interphase cells, 1.9% (0% to 3%) of the cells in the B-cell lines showed one fluorescent spot and 1.1% (0% to 2.9%) of the cells in the T-cell lines. These values do not differ from normal values. However, the possibility that normal cells were selected when the T- and B-cell lines were established could not be excluded. Therefore, peripheral blood cells were obtained, separated according to surface markers specific for lymphoid and myeloid cell lineage with a cell sorter, and analyzed for the expression of monosomy 7 by FISH. Antibodies recognizing T cells (CD3), B cells (CD20), natural killer (NK) cells (CD57), monocytes and granulocytes (low and high expression of CD11b antigen), and myeloid progenitors (CD33) were used to separate cells. The expression of monosomy 7 in the T cells, NK cells, and B cells did not differ from control values. These results in the lymphoid subpopulations are in stark contrast with the observations in the myeloid populations; the percentage of cells with monosomy 7 ranged from 9% to 78% (controls: 6% ± 2%) in cells with low CD11b expression, 20% to 89% in cells with a high expression of the CD11b antigen (controls: 7% ± 3%), and 23% to 91% in the CD33 positive cells (controls: 5% ± 3%). The results of this study suggest that monosomy 7 does not usually affect lymphoid subpopulations but is restricted to committed progenitor cells with the capacity to differentiate into mature myeloid cells.

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