Clock genes and environmental cues coordinate Anopheles pheromone synthesis, swarming, and mating

Guandong Wang; Joel Vega-Rodríguez; Abdoulaye Diabate; Jingnan Liu; Chunlai Cui; Charles Nignan; Ling Dong; Fang Li; Cheick Oumar Ouedrago; Abdoul Malik Bandaogo; Péguédwindé Simon Sawadogo; Hamidou Maiga; Thiago Luiz Alvese Silva; Tales Vicari Pascini; Sibao Wang; Marcelo Jacobs-Lorena

doi:10.1126/science.abd4359

Clock genes and environmental cues coordinate Anopheles pheromone synthesis, swarming, and mating

Guandong Wang, Joel Vega-Rodríguez, Abdoulaye Diabate, Jingnan Liu, Chunlai Cui, Charles Nignan, Ling Dong, Fang Li, Cheick Oumar Ouedrago, Abdoul Malik Bandaogo, Péguédwindé Simon Sawadogo, Hamidou Maiga, Thiago Luiz Alvese Silva, Tales Vicari Pascini, Sibao Wang, Marcelo Jacobs-Lorena

Bloomberg School of Public Health

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Anopheles mating is initiated by the swarming of males at dusk followed by females flying into the swarm. Here, we show that mosquito swarming and mating are coordinately guided by clock genes, light, and temperature. Transcriptome analysis shows up-regulation of the clock genes period (per) and timeless (tim) in the head of field-caught swarming Anopheles coluzzii males. Knockdown of per and tim expression affects Anopheles gambiae s.s. and Anopheles stephensi male mating in the laboratory, and it reduces male An. coluzzii swarming and mating under semifield conditions. Light and temperature affect mosquito mating, possibly by modulating per and/or tim expression. Moreover, the desaturase gene desat1 is up-regulated and rhythmically expressed in the heads of swarming males and regulates the production of cuticular hydrocarbons, including heptacosane, which stimulates mating activity.

Original language	English (US)
Pages (from-to)	411-415
Number of pages	5
Journal	Science
Volume	371
Issue number	6527
DOIs	https://doi.org/10.1126/science.abd4359
State	Published - Jan 22 2021

ASJC Scopus subject areas

General

Access to Document

10.1126/science.abd4359

Cite this

Wang, G., Vega-Rodríguez, J., Diabate, A., Liu, J., Cui, C., Nignan, C., Dong, L., Li, F., Ouedrago, C. O., Bandaogo, A. M., Sawadogo, P. S., Maiga, H., Silva, T. L. A., Pascini, T. V., Wang, S., & Jacobs-Lorena, M. (2021). Clock genes and environmental cues coordinate Anopheles pheromone synthesis, swarming, and mating. Science, 371(6527), 411-415. https://doi.org/10.1126/science.abd4359

Wang, G, Vega-Rodríguez, J, Diabate, A, Liu, J, Cui, C, Nignan, C, Dong, L, Li, F, Ouedrago, CO, Bandaogo, AM, Sawadogo, PS, Maiga, H, Silva, TLA, Pascini, TV, Wang, S & Jacobs-Lorena, M 2021, 'Clock genes and environmental cues coordinate Anopheles pheromone synthesis, swarming, and mating', Science, vol. 371, no. 6527, pp. 411-415. https://doi.org/10.1126/science.abd4359

@article{5abde5cf058b498ba6fc7475f4963c7a,

title = "Clock genes and environmental cues coordinate Anopheles pheromone synthesis, swarming, and mating",

abstract = "Anopheles mating is initiated by the swarming of males at dusk followed by females flying into the swarm. Here, we show that mosquito swarming and mating are coordinately guided by clock genes, light, and temperature. Transcriptome analysis shows up-regulation of the clock genes period (per) and timeless (tim) in the head of field-caught swarming Anopheles coluzzii males. Knockdown of per and tim expression affects Anopheles gambiae s.s. and Anopheles stephensi male mating in the laboratory, and it reduces male An. coluzzii swarming and mating under semifield conditions. Light and temperature affect mosquito mating, possibly by modulating per and/or tim expression. Moreover, the desaturase gene desat1 is up-regulated and rhythmically expressed in the heads of swarming males and regulates the production of cuticular hydrocarbons, including heptacosane, which stimulates mating activity.",

author = "Guandong Wang and Joel Vega-Rodr{\'i}guez and Abdoulaye Diabate and Jingnan Liu and Chunlai Cui and Charles Nignan and Ling Dong and Fang Li and Ouedrago, {Cheick Oumar} and Bandaogo, {Abdoul Malik} and Sawadogo, {P{\'e}gu{\'e}dwind{\'e} Simon} and Hamidou Maiga and Silva, {Thiago Luiz Alvese} and Pascini, {Tales Vicari} and Sibao Wang and Marcelo Jacobs-Lorena",

year = "2021",

month = jan,

day = "22",

doi = "10.1126/science.abd4359",

language = "English (US)",

volume = "371",

pages = "411--415",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "6527",

}

TY - JOUR

T1 - Clock genes and environmental cues coordinate Anopheles pheromone synthesis, swarming, and mating

AU - Wang, Guandong

AU - Vega-Rodríguez, Joel

AU - Diabate, Abdoulaye

AU - Liu, Jingnan

AU - Cui, Chunlai

AU - Nignan, Charles

AU - Dong, Ling

AU - Li, Fang

AU - Ouedrago, Cheick Oumar

AU - Bandaogo, Abdoul Malik

AU - Sawadogo, Péguédwindé Simon

AU - Maiga, Hamidou

AU - Silva, Thiago Luiz Alvese

AU - Pascini, Tales Vicari

AU - Wang, Sibao

AU - Jacobs-Lorena, Marcelo

PY - 2021/1/22

Y1 - 2021/1/22

N2 - Anopheles mating is initiated by the swarming of males at dusk followed by females flying into the swarm. Here, we show that mosquito swarming and mating are coordinately guided by clock genes, light, and temperature. Transcriptome analysis shows up-regulation of the clock genes period (per) and timeless (tim) in the head of field-caught swarming Anopheles coluzzii males. Knockdown of per and tim expression affects Anopheles gambiae s.s. and Anopheles stephensi male mating in the laboratory, and it reduces male An. coluzzii swarming and mating under semifield conditions. Light and temperature affect mosquito mating, possibly by modulating per and/or tim expression. Moreover, the desaturase gene desat1 is up-regulated and rhythmically expressed in the heads of swarming males and regulates the production of cuticular hydrocarbons, including heptacosane, which stimulates mating activity.

AB - Anopheles mating is initiated by the swarming of males at dusk followed by females flying into the swarm. Here, we show that mosquito swarming and mating are coordinately guided by clock genes, light, and temperature. Transcriptome analysis shows up-regulation of the clock genes period (per) and timeless (tim) in the head of field-caught swarming Anopheles coluzzii males. Knockdown of per and tim expression affects Anopheles gambiae s.s. and Anopheles stephensi male mating in the laboratory, and it reduces male An. coluzzii swarming and mating under semifield conditions. Light and temperature affect mosquito mating, possibly by modulating per and/or tim expression. Moreover, the desaturase gene desat1 is up-regulated and rhythmically expressed in the heads of swarming males and regulates the production of cuticular hydrocarbons, including heptacosane, which stimulates mating activity.

UR - http://www.scopus.com/inward/record.url?scp=85099926125&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85099926125&partnerID=8YFLogxK

U2 - 10.1126/science.abd4359

DO - 10.1126/science.abd4359

M3 - Article

C2 - 33479155

AN - SCOPUS:85099926125

SN - 0036-8075

VL - 371

SP - 411

EP - 415

JO - Science

JF - Science

IS - 6527

ER -

Clock genes and environmental cues coordinate Anopheles pheromone synthesis, swarming, and mating

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this