TY - JOUR
T1 - Clinicopathologic and genetic characterization of traditional serrated adenomas of the colon
AU - Fu, Baojin
AU - Yachida, Shinichi
AU - Morgan, Richard
AU - Zhong, Yi
AU - Montgomery, Elizabeth A.
AU - Iacobuzio-Donahue, Christine A.
PY - 2012/9
Y1 - 2012/9
N2 - Traditional serrated adenomas (TSAs) are a type of colorectal polyp with neoplastic potential. Immunohistochemical analysis and sequencing were performed on 24 TSAs from 23 patients to characterize the molecular genetics of TSAs. Abnormal Ki-67 and p53 labeling were observed in 7 (29%) of 24 and 6 (25%) of 24 TSAs, respectively; both types were significantly associated with the presence of conventional epithelial dysplasia (P = .0005 and P = .0001, respectively). Activating KRAS mutation was identified in 11 TSAs (46%) and was mutually exclusive with activating BRAF mutations, which were seen in 7 TSAs (29%). Abnormal p53 nuclear labeling in a TSA was significantly associated with BRAF mutation status (P = .04), whereas no relationship was found for β-catenin labeling patterns. The overall morphologic features of TSA do not correlate with the genetic status of the KRAS and BRAF genes. However, conventional epithelial dysplasia and abnormal p53 labeling in a TSA are seen more often in the setting of BRAF mutation. Copyright
AB - Traditional serrated adenomas (TSAs) are a type of colorectal polyp with neoplastic potential. Immunohistochemical analysis and sequencing were performed on 24 TSAs from 23 patients to characterize the molecular genetics of TSAs. Abnormal Ki-67 and p53 labeling were observed in 7 (29%) of 24 and 6 (25%) of 24 TSAs, respectively; both types were significantly associated with the presence of conventional epithelial dysplasia (P = .0005 and P = .0001, respectively). Activating KRAS mutation was identified in 11 TSAs (46%) and was mutually exclusive with activating BRAF mutations, which were seen in 7 TSAs (29%). Abnormal p53 nuclear labeling in a TSA was significantly associated with BRAF mutation status (P = .04), whereas no relationship was found for β-catenin labeling patterns. The overall morphologic features of TSA do not correlate with the genetic status of the KRAS and BRAF genes. However, conventional epithelial dysplasia and abnormal p53 labeling in a TSA are seen more often in the setting of BRAF mutation. Copyright
KW - BRAF
KW - Colon polyp
KW - KRAS
KW - Traditional serrated adenoma
KW - p53
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U2 - 10.1309/AJCPVT7LC4CRPZSK
DO - 10.1309/AJCPVT7LC4CRPZSK
M3 - Article
C2 - 22912351
AN - SCOPUS:84865408888
SN - 0002-9173
VL - 138
SP - 356
EP - 366
JO - American journal of clinical pathology
JF - American journal of clinical pathology
IS - 3
ER -