Clinico-pathologic features of fatal disease attributed to new variants of endotheliotropic herpesviruses in two Asian elephants (Elephas maximus)

M. M. Garner, K. Helmick, J. Ochsenreiter, L. K. Richman, E. Latimer, A. G. Wise, R. K. Maes, M. Kiupel, R. W. Nordhausen, Jianchao Zong, Gary Selwyn Hayward

Research output: Contribution to journalArticle

Abstract

The first herpesviruses described in association with serious elephant disease were referred to as endotheliotropic herpesviruses (EEHV) because of their ability to infect capillary endothelial cells and cause potentially fatal disease. Two related viruses, EEHV1 and EEHV2, have been described based on genetic composition. This report describes the similarities and differences in clinicopathologic features of 2 cases of fatal endotheliotropic herpesvirus infections in Asian elephants caused by a previously unrecognized virus within the betaherpesvirus subfamily. EEHV3 is markedly divergent from the 2 previously studied fatal probosciviruses, based on polymerase chain reaction sequence analysis of 2 segments of the viral genome. In addition to ascites, widespread visceral edema, petechiae, and capillary damage previously reported, important findings with EEHV3 infection were the presence of grossly visible renal medullary hemorrhage, a tropism for larger veins and arteries in various tissues, relatively high density of renal herpetic inclusions, and involvement of the retinal vessels. These findings indicate a less selective organ tropism, and this may confer a higher degree of virulence for EEHV3.

Original languageEnglish (US)
Pages (from-to)97-104
Number of pages8
JournalVeterinary Pathology
Volume46
Issue number1
DOIs
StatePublished - Jan 2009

Fingerprint

Elephas maximus
tropisms
Tropism
Herpesviridae
Proboscivirus
kidneys
Viruses
Kidney
Herpesviridae Infections
Retinal Vessels
viruses
Purpura
Viral Genome
ascites
Elephantidae
Ascites
edema
arteries
endothelial cells
hemorrhage

Keywords

  • Electron microscopy
  • Elephants
  • Endotheliotropic herpesviruses
  • Herpesvirus
  • Histopa- Thology
  • Polymerase chain reaction

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Clinico-pathologic features of fatal disease attributed to new variants of endotheliotropic herpesviruses in two Asian elephants (Elephas maximus). / Garner, M. M.; Helmick, K.; Ochsenreiter, J.; Richman, L. K.; Latimer, E.; Wise, A. G.; Maes, R. K.; Kiupel, M.; Nordhausen, R. W.; Zong, Jianchao; Hayward, Gary Selwyn.

In: Veterinary Pathology, Vol. 46, No. 1, 01.2009, p. 97-104.

Research output: Contribution to journalArticle

Garner, MM, Helmick, K, Ochsenreiter, J, Richman, LK, Latimer, E, Wise, AG, Maes, RK, Kiupel, M, Nordhausen, RW, Zong, J & Hayward, GS 2009, 'Clinico-pathologic features of fatal disease attributed to new variants of endotheliotropic herpesviruses in two Asian elephants (Elephas maximus)', Veterinary Pathology, vol. 46, no. 1, pp. 97-104. https://doi.org/10.1354/vp.46-1-97
Garner, M. M. ; Helmick, K. ; Ochsenreiter, J. ; Richman, L. K. ; Latimer, E. ; Wise, A. G. ; Maes, R. K. ; Kiupel, M. ; Nordhausen, R. W. ; Zong, Jianchao ; Hayward, Gary Selwyn. / Clinico-pathologic features of fatal disease attributed to new variants of endotheliotropic herpesviruses in two Asian elephants (Elephas maximus). In: Veterinary Pathology. 2009 ; Vol. 46, No. 1. pp. 97-104.
@article{4488c6f621c64073897b803b5f38c798,
title = "Clinico-pathologic features of fatal disease attributed to new variants of endotheliotropic herpesviruses in two Asian elephants (Elephas maximus)",
abstract = "The first herpesviruses described in association with serious elephant disease were referred to as endotheliotropic herpesviruses (EEHV) because of their ability to infect capillary endothelial cells and cause potentially fatal disease. Two related viruses, EEHV1 and EEHV2, have been described based on genetic composition. This report describes the similarities and differences in clinicopathologic features of 2 cases of fatal endotheliotropic herpesvirus infections in Asian elephants caused by a previously unrecognized virus within the betaherpesvirus subfamily. EEHV3 is markedly divergent from the 2 previously studied fatal probosciviruses, based on polymerase chain reaction sequence analysis of 2 segments of the viral genome. In addition to ascites, widespread visceral edema, petechiae, and capillary damage previously reported, important findings with EEHV3 infection were the presence of grossly visible renal medullary hemorrhage, a tropism for larger veins and arteries in various tissues, relatively high density of renal herpetic inclusions, and involvement of the retinal vessels. These findings indicate a less selective organ tropism, and this may confer a higher degree of virulence for EEHV3.",
keywords = "Electron microscopy, Elephants, Endotheliotropic herpesviruses, Herpesvirus, Histopa- Thology, Polymerase chain reaction",
author = "Garner, {M. M.} and K. Helmick and J. Ochsenreiter and Richman, {L. K.} and E. Latimer and Wise, {A. G.} and Maes, {R. K.} and M. Kiupel and Nordhausen, {R. W.} and Jianchao Zong and Hayward, {Gary Selwyn}",
year = "2009",
month = "1",
doi = "10.1354/vp.46-1-97",
language = "English (US)",
volume = "46",
pages = "97--104",
journal = "Veterinary Pathology",
issn = "0300-9858",
publisher = "SAGE Publications Ltd",
number = "1",

}

TY - JOUR

T1 - Clinico-pathologic features of fatal disease attributed to new variants of endotheliotropic herpesviruses in two Asian elephants (Elephas maximus)

AU - Garner, M. M.

AU - Helmick, K.

AU - Ochsenreiter, J.

AU - Richman, L. K.

AU - Latimer, E.

AU - Wise, A. G.

AU - Maes, R. K.

AU - Kiupel, M.

AU - Nordhausen, R. W.

AU - Zong, Jianchao

AU - Hayward, Gary Selwyn

PY - 2009/1

Y1 - 2009/1

N2 - The first herpesviruses described in association with serious elephant disease were referred to as endotheliotropic herpesviruses (EEHV) because of their ability to infect capillary endothelial cells and cause potentially fatal disease. Two related viruses, EEHV1 and EEHV2, have been described based on genetic composition. This report describes the similarities and differences in clinicopathologic features of 2 cases of fatal endotheliotropic herpesvirus infections in Asian elephants caused by a previously unrecognized virus within the betaherpesvirus subfamily. EEHV3 is markedly divergent from the 2 previously studied fatal probosciviruses, based on polymerase chain reaction sequence analysis of 2 segments of the viral genome. In addition to ascites, widespread visceral edema, petechiae, and capillary damage previously reported, important findings with EEHV3 infection were the presence of grossly visible renal medullary hemorrhage, a tropism for larger veins and arteries in various tissues, relatively high density of renal herpetic inclusions, and involvement of the retinal vessels. These findings indicate a less selective organ tropism, and this may confer a higher degree of virulence for EEHV3.

AB - The first herpesviruses described in association with serious elephant disease were referred to as endotheliotropic herpesviruses (EEHV) because of their ability to infect capillary endothelial cells and cause potentially fatal disease. Two related viruses, EEHV1 and EEHV2, have been described based on genetic composition. This report describes the similarities and differences in clinicopathologic features of 2 cases of fatal endotheliotropic herpesvirus infections in Asian elephants caused by a previously unrecognized virus within the betaherpesvirus subfamily. EEHV3 is markedly divergent from the 2 previously studied fatal probosciviruses, based on polymerase chain reaction sequence analysis of 2 segments of the viral genome. In addition to ascites, widespread visceral edema, petechiae, and capillary damage previously reported, important findings with EEHV3 infection were the presence of grossly visible renal medullary hemorrhage, a tropism for larger veins and arteries in various tissues, relatively high density of renal herpetic inclusions, and involvement of the retinal vessels. These findings indicate a less selective organ tropism, and this may confer a higher degree of virulence for EEHV3.

KW - Electron microscopy

KW - Elephants

KW - Endotheliotropic herpesviruses

KW - Herpesvirus

KW - Histopa- Thology

KW - Polymerase chain reaction

UR - http://www.scopus.com/inward/record.url?scp=63249114896&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=63249114896&partnerID=8YFLogxK

U2 - 10.1354/vp.46-1-97

DO - 10.1354/vp.46-1-97

M3 - Article

C2 - 19112123

AN - SCOPUS:63249114896

VL - 46

SP - 97

EP - 104

JO - Veterinary Pathology

JF - Veterinary Pathology

SN - 0300-9858

IS - 1

ER -