Clinical utility of cytomegalovirus viral load in bronchoalveolar lavage in lung transplant recipients

Roy F. Chemaly, Belinda Yen-Lieberman, Jeffrey Chapman, Amy Reilly, B. Nebiyou Bekele, Steven M. Gordon, Gary W. Procop, Nabin Shrestha, Carlos M. Isada, Malcolm DeCamp, Robin Avery

Research output: Contribution to journalArticle

Abstract

The utility of cytomegalovirus (CMV) viral load (VL) by quantitative hybrid capture assay (Q-HCA) was investigated in bronchoalveolar lavage (BAL) from lung transplant recipients and compared with BAL cultures and blood VL. Forty-three consecutive BAL samples from 27 lung transplant recipients were analyzed. All samples had shell vial (SV) cultures in addition to Q-HCA. Histopathology was done on all lung tissues, and immunohistochemistry (IMC) in those with positive CMV cultures. Fifteen (56%) lung transplant recipients had both positive BAL SV cultures and BAL VL. Five of 15 had CMV pneumonitis with a VL in BAL >500 000 copies/mL (mean: 1638 450). Ten patients without CMV pneumonitis had VL in BAL

Original languageEnglish (US)
Pages (from-to)544-548
Number of pages5
JournalAmerican Journal of Transplantation
Volume5
Issue number3
DOIs
StatePublished - Mar 2005
Externally publishedYes

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Bronchoalveolar Lavage
Viral Load
Cytomegalovirus
Lung
Pneumonia
Transplant Recipients
Immunohistochemistry

Keywords

  • BAL
  • CMV
  • Lung transplant
  • Viral load

ASJC Scopus subject areas

  • Immunology

Cite this

Clinical utility of cytomegalovirus viral load in bronchoalveolar lavage in lung transplant recipients. / Chemaly, Roy F.; Yen-Lieberman, Belinda; Chapman, Jeffrey; Reilly, Amy; Bekele, B. Nebiyou; Gordon, Steven M.; Procop, Gary W.; Shrestha, Nabin; Isada, Carlos M.; DeCamp, Malcolm; Avery, Robin.

In: American Journal of Transplantation, Vol. 5, No. 3, 03.2005, p. 544-548.

Research output: Contribution to journalArticle

Chemaly, RF, Yen-Lieberman, B, Chapman, J, Reilly, A, Bekele, BN, Gordon, SM, Procop, GW, Shrestha, N, Isada, CM, DeCamp, M & Avery, R 2005, 'Clinical utility of cytomegalovirus viral load in bronchoalveolar lavage in lung transplant recipients', American Journal of Transplantation, vol. 5, no. 3, pp. 544-548. https://doi.org/10.1111/j.1600-6143.2005.00747.x
Chemaly, Roy F. ; Yen-Lieberman, Belinda ; Chapman, Jeffrey ; Reilly, Amy ; Bekele, B. Nebiyou ; Gordon, Steven M. ; Procop, Gary W. ; Shrestha, Nabin ; Isada, Carlos M. ; DeCamp, Malcolm ; Avery, Robin. / Clinical utility of cytomegalovirus viral load in bronchoalveolar lavage in lung transplant recipients. In: American Journal of Transplantation. 2005 ; Vol. 5, No. 3. pp. 544-548.
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