Clinical significance of K-ras oncogene activation in ampullary neoplasms

C. H. Chung, R. E. Wilentz, M. M. Polak, T. B. Ramsoekh, L. A. Noorduyn, D. J. Gouma, K. Huibregtse, G. J A Offerhaus, R. J C Slebos

Research output: Contribution to journalArticle

Abstract

Aims - To investigate the prevalence of K-ras codon 12 point mutations in ampullary neoplasms, to explore their clinical usefulness, and to test whether the detection of these mutations could be used to identify ampullary malignancies at an early stage. Methods - Forty one tumour specimens from 28 patients with ampullary neoplasms were analysed for activating point mutations in K-ras codon 12 using a sensitive polymerase chain reaction (PCR) based assay. Results - Eleven (39%) of the 28 primary tumours harboured point mutations in K-ras. Mutations were identified in seven (41%) of the 17 carcinomas and four (36%) of the 11 adenomas. Four of the possible six permutations in codon 12 were found in these 11 samples. This spectrum of mutations is different from pancreatic carcinoma but resembles that of colorectal neoplasms. Cytological brush specimens were available in 11 cases, and in all of these specimens, the K-ras status in the primary tumour and brush specimens was identical. Conclusions - K-ras codon 12 point mutations occur in about 40% of ampullary neoplasms at a relatively early stage in tumorigenesis. The pattern of mutations in these tumours resembles that of the adenoma-carcinoma sequence in the colorectum. These results indicate that ampullary neoplasms can be detected at an early stage by searching for genetic alterations in the K-ras oncogene in cytological brush specimens.

Original languageEnglish (US)
Pages (from-to)460-464
Number of pages5
JournalJournal of Clinical Pathology
Volume49
Issue number6
StatePublished - 1996
Externally publishedYes

Fingerprint

ras Genes
Point Mutation
Codon
Neoplasms
Mutation
Adenoma
Carcinoma
Colorectal Neoplasms
Carcinogenesis
Polymerase Chain Reaction

Keywords

  • Ampullary neoplasms
  • Brush cytology
  • Endoscopic retrograde cholangiopancreatography
  • K-ras
  • Point mutation

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Chung, C. H., Wilentz, R. E., Polak, M. M., Ramsoekh, T. B., Noorduyn, L. A., Gouma, D. J., ... Slebos, R. J. C. (1996). Clinical significance of K-ras oncogene activation in ampullary neoplasms. Journal of Clinical Pathology, 49(6), 460-464.

Clinical significance of K-ras oncogene activation in ampullary neoplasms. / Chung, C. H.; Wilentz, R. E.; Polak, M. M.; Ramsoekh, T. B.; Noorduyn, L. A.; Gouma, D. J.; Huibregtse, K.; Offerhaus, G. J A; Slebos, R. J C.

In: Journal of Clinical Pathology, Vol. 49, No. 6, 1996, p. 460-464.

Research output: Contribution to journalArticle

Chung, CH, Wilentz, RE, Polak, MM, Ramsoekh, TB, Noorduyn, LA, Gouma, DJ, Huibregtse, K, Offerhaus, GJA & Slebos, RJC 1996, 'Clinical significance of K-ras oncogene activation in ampullary neoplasms', Journal of Clinical Pathology, vol. 49, no. 6, pp. 460-464.
Chung CH, Wilentz RE, Polak MM, Ramsoekh TB, Noorduyn LA, Gouma DJ et al. Clinical significance of K-ras oncogene activation in ampullary neoplasms. Journal of Clinical Pathology. 1996;49(6):460-464.
Chung, C. H. ; Wilentz, R. E. ; Polak, M. M. ; Ramsoekh, T. B. ; Noorduyn, L. A. ; Gouma, D. J. ; Huibregtse, K. ; Offerhaus, G. J A ; Slebos, R. J C. / Clinical significance of K-ras oncogene activation in ampullary neoplasms. In: Journal of Clinical Pathology. 1996 ; Vol. 49, No. 6. pp. 460-464.
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AU - Wilentz, R. E.

AU - Polak, M. M.

AU - Ramsoekh, T. B.

AU - Noorduyn, L. A.

AU - Gouma, D. J.

AU - Huibregtse, K.

AU - Offerhaus, G. J A

AU - Slebos, R. J C

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