Abstract
Aims - To investigate the prevalence of K-ras codon 12 point mutations in ampullary neoplasms, to explore their clinical usefulness, and to test whether the detection of these mutations could be used to identify ampullary malignancies at an early stage. Methods - Forty one tumour specimens from 28 patients with ampullary neoplasms were analysed for activating point mutations in K-ras codon 12 using a sensitive polymerase chain reaction (PCR) based assay. Results - Eleven (39%) of the 28 primary tumours harboured point mutations in K-ras. Mutations were identified in seven (41%) of the 17 carcinomas and four (36%) of the 11 adenomas. Four of the possible six permutations in codon 12 were found in these 11 samples. This spectrum of mutations is different from pancreatic carcinoma but resembles that of colorectal neoplasms. Cytological brush specimens were available in 11 cases, and in all of these specimens, the K-ras status in the primary tumour and brush specimens was identical. Conclusions - K-ras codon 12 point mutations occur in about 40% of ampullary neoplasms at a relatively early stage in tumorigenesis. The pattern of mutations in these tumours resembles that of the adenoma-carcinoma sequence in the colorectum. These results indicate that ampullary neoplasms can be detected at an early stage by searching for genetic alterations in the K-ras oncogene in cytological brush specimens.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 460-464 |
| Number of pages | 5 |
| Journal | Journal of Clinical Pathology |
| Volume | 49 |
| Issue number | 6 |
| State | Published - 1996 |
| Externally published | Yes |
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Keywords
- Ampullary neoplasms
- Brush cytology
- Endoscopic retrograde cholangiopancreatography
- K-ras
- Point mutation
ASJC Scopus subject areas
- Pathology and Forensic Medicine
Cite this
Clinical significance of K-ras oncogene activation in ampullary neoplasms. / Chung, C. H.; Wilentz, R. E.; Polak, M. M.; Ramsoekh, T. B.; Noorduyn, L. A.; Gouma, D. J.; Huibregtse, K.; Offerhaus, G. J A; Slebos, R. J C.
In: Journal of Clinical Pathology, Vol. 49, No. 6, 1996, p. 460-464.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Clinical significance of K-ras oncogene activation in ampullary neoplasms
AU - Chung, C. H.
AU - Wilentz, R. E.
AU - Polak, M. M.
AU - Ramsoekh, T. B.
AU - Noorduyn, L. A.
AU - Gouma, D. J.
AU - Huibregtse, K.
AU - Offerhaus, G. J A
AU - Slebos, R. J C
PY - 1996
Y1 - 1996
N2 - Aims - To investigate the prevalence of K-ras codon 12 point mutations in ampullary neoplasms, to explore their clinical usefulness, and to test whether the detection of these mutations could be used to identify ampullary malignancies at an early stage. Methods - Forty one tumour specimens from 28 patients with ampullary neoplasms were analysed for activating point mutations in K-ras codon 12 using a sensitive polymerase chain reaction (PCR) based assay. Results - Eleven (39%) of the 28 primary tumours harboured point mutations in K-ras. Mutations were identified in seven (41%) of the 17 carcinomas and four (36%) of the 11 adenomas. Four of the possible six permutations in codon 12 were found in these 11 samples. This spectrum of mutations is different from pancreatic carcinoma but resembles that of colorectal neoplasms. Cytological brush specimens were available in 11 cases, and in all of these specimens, the K-ras status in the primary tumour and brush specimens was identical. Conclusions - K-ras codon 12 point mutations occur in about 40% of ampullary neoplasms at a relatively early stage in tumorigenesis. The pattern of mutations in these tumours resembles that of the adenoma-carcinoma sequence in the colorectum. These results indicate that ampullary neoplasms can be detected at an early stage by searching for genetic alterations in the K-ras oncogene in cytological brush specimens.
AB - Aims - To investigate the prevalence of K-ras codon 12 point mutations in ampullary neoplasms, to explore their clinical usefulness, and to test whether the detection of these mutations could be used to identify ampullary malignancies at an early stage. Methods - Forty one tumour specimens from 28 patients with ampullary neoplasms were analysed for activating point mutations in K-ras codon 12 using a sensitive polymerase chain reaction (PCR) based assay. Results - Eleven (39%) of the 28 primary tumours harboured point mutations in K-ras. Mutations were identified in seven (41%) of the 17 carcinomas and four (36%) of the 11 adenomas. Four of the possible six permutations in codon 12 were found in these 11 samples. This spectrum of mutations is different from pancreatic carcinoma but resembles that of colorectal neoplasms. Cytological brush specimens were available in 11 cases, and in all of these specimens, the K-ras status in the primary tumour and brush specimens was identical. Conclusions - K-ras codon 12 point mutations occur in about 40% of ampullary neoplasms at a relatively early stage in tumorigenesis. The pattern of mutations in these tumours resembles that of the adenoma-carcinoma sequence in the colorectum. These results indicate that ampullary neoplasms can be detected at an early stage by searching for genetic alterations in the K-ras oncogene in cytological brush specimens.
KW - Ampullary neoplasms
KW - Brush cytology
KW - Endoscopic retrograde cholangiopancreatography
KW - K-ras
KW - Point mutation
UR - http://www.scopus.com/inward/record.url?scp=0029920056&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029920056&partnerID=8YFLogxK
M3 - Article
C2 - 8763258
AN - SCOPUS:0029920056
VL - 49
SP - 460
EP - 464
JO - Journal of Clinical Pathology
JF - Journal of Clinical Pathology
SN - 0021-9746
IS - 6
ER -